增强绿色荧光蛋白,EGFP
1)EGFP增强绿色荧光蛋白
1.Construction of Eukaryotic Expression Vector Containing shRNA Targeting EGFP靶向增强绿色荧光蛋白shRNA的真核表达载体构建
2.[Objective] To transfect neural stem cells with adenovirus vector of BDNF and EGFP,and also to investigate the expression of BDNF and EGFP in transfected neural stem cells.方法通过RT-PCR从大鼠海马中获得BDNF基因,通过基因克隆、HEK293包装,获得含增强绿色荧光蛋白基因的重组腺病毒表达载体pAd-BDNF-EGFP,将其感染原代培养的神经干细胞,观察EGFP及BDNF2种基因的表达,镜下测定转染率,并检测RT-PCR产物,证实BDNF的存在。
3.Objective: To construct secretion eukaryotic expression vector pSecTag-EGFP and study the transfection efficiency in chicken embryonic fibroblast cells,in establishing the foundation for the aim gene expression in chicken embryonic fibroblast cells.目的构建含报告基因增强绿色荧光蛋白(EGFP)的分泌型真核表达载体pSecTag-EGFP,用以研究pSecTag/HygroB转染鸡胚胎成纤维细胞的转染效率,为目的基因的成功表达奠定基础。
英文短句/例句

1.Construction of Eukaryotic Expression Vector Containing shRNA Targeting EGFP靶向增强绿色荧光蛋白shRNA的真核表达载体构建
2.Construction,transfection and activity identification of eukaryotic expression vector of human PRKCB1 containing enhanced green fluorescence protein gene携带增强绿色荧光蛋白基因的人PRKCB1真核表达载体构建、转染及活性鉴定
3.Effect of Dual Promoter on Expression of Enhancement Green Fluorescent Protein双启动子对增强型绿色荧光蛋白表达的影响
4.Expression of Enhanced Green Fluorescent Protein (eGFP) in Synechocystis sp. PCC6803增强型绿色荧光蛋白在集胞藻6803中的表达
5.Breeding and EGFP Labeling of High Efficient Anthracene-Degrading Bacteria;蒽高效降解菌的选育及其增强型绿色荧光蛋白标记
6.Construction and Expression of the Enhanced Green Fluorescent Protein Reporter Gene Eucaryotic Vcetor Carrying BMP2 Gene;含增强型绿色荧光蛋白的BMP2真核载体的构建和表达
7.Construction of the mutant of human amyloid precursor protein and enhanced fluorescence protein fusion gene突变型人APP基因与增强型绿色荧光蛋白基因融合载体的构建
8.Establishment of bladder cancer cell line expressing enhanced green fluorescent protein gene稳定高表达增强型绿色荧光蛋白基因膀胱癌细胞株的构建
9.In vitro Differentiation of Enhanced Green Fluorescence Protein Gene (EGFP) Transfected Porcine Amniotic Fluid-derived Stem Cells转增强型绿色荧光蛋白基因(EGFP)猪羊水干细胞的体外分化
10.Tracking Marrow Mesenchymal Stem Cells by Transfection with Enhanced Green Fluorescent Protein增强型绿色荧光蛋白-C_1转染示踪骨髓间充质干细胞
11.Construction and identification of a screening vector using enhanced green fluorescent protein(EGFP) as an indicator利用增强型绿色荧光蛋白基因作筛选标记克隆载体的构建及鉴定
12.Construction and Expression of Enhanced Green Fluorescent Protein and FXR1 Co-expression VectorFXR1基因与增强型绿色荧光蛋白融合载体的构建及表达
13.Study on liposome-mediated enhanced green fluorescent protein expression in murine Müller cells脂质体介导增强型绿色荧光蛋白基因在鼠视网膜Müller细胞中的表达
14.Construction, Expression of Fusion Protein A-enhanced Green Fluorescent Protein (PA-EGFP) and Its Application to Immunoassay;蛋白A-增强型绿色荧光蛋白融合基因的构建、表达及其在免疫测定中的应用研究
15.The Production of Transgenic Sheep Embryos Transfected with Green Fluorescence Protein and Augmenter of Liver Regeneration Genes用绿色荧光蛋白和肝再生增强因子基因进行转基因绵羊胚胎的研究
16.Experimental Study of Transfecting EGFP Gene into Osteoblasts Derived from Rabbit Marrow Stromal Cells;增强型绿色荧光蛋白基因转染兔骨髓基质细胞诱导的成骨样细胞的实验研究
17.Heterogenous Expression of Enhanced Green Flurescent Protein (EGFP) Gene in Schistosoma Japonicum and in Vitro Primary Cultured Cells of Schistosomula;增强型绿色荧光蛋白(EGFP)基因在日本血吸虫体内及原代培养细胞内的异源表达
18.Constrction of Shuttle Vector Plasmid Expressing EGFP of Pseudorabies Virus-Fa Strain;含增强型绿色荧光蛋白(EGFP)的伪狂犬病病毒Fa株转移载体质粒的构建
相关短句/例句

Enhanced green fluorescent protein增强绿色荧光蛋白
1.Rescue of a recombinant vesicular stomatitis virus expressing enhanced green fluorescent protein;表达增强绿色荧光蛋白重组水疱性口炎病毒印第安纳株的构建
2.Objective:To construct a retroviral vector carrying both enhanced green fluorescent protein(EGFP) and human insulin gene for transfecting the bone marrow mesenchymal stem cells (BMSCs),and to observe the treatment effect of transfected BMSCs after transplanted into diabetes mice.目的:构建含增强绿色荧光蛋白(EGFP)及胰岛素融合基因的重组质粒,转染小鼠骨髓间充质干细胞(BMSCs),观察转染融合基因后BMSCs移植治疗糖尿病模型小鼠的治疗效果。
3.【Objective】 To investigate the influence of enhanced green fluorescent protein gene mediated by recombinant adeno-associated virus vector with different serotypes on the transfection efficiency and cytotoxicity to canine bone marrow mesenchymal stem cells in vitro.【目的】研究不同血清型腺相关病毒(rAAV)载体介导的增强绿色荧光蛋白基因(EGFP)对体外培养犬骨髓间充质干细胞(MSC)的转染效率及其毒性作用。
3)Enhanced green fluorescent protein(EGFP)增强型绿色荧光蛋白
1.Objective Making the fusion protein of IgG-binding peptide with enhanced green fluorescent protein(EGFP) and determining its bioactivity.目的构建、表达增强型绿色荧光蛋白(EGFP)与IgG抗体亲和肽融合蛋白,并对其生物学功能进行研究。
2.Plasmid DNA was the enhanced green fluorescent protein(EGFP)vector.以质粒DNA为增强型绿色荧光蛋白 (EGFP)的载体 ,对处于快速增殖期的 8株猪胚胎生殖 (EG)细胞 (4~ 9代 )进行转基因试验。
3.The coding sequence of enhanced green fluorescent protein(EGFP)was cloned into the promoterless vector pLAFR6,generated the reporter plasmid pLZY.将增强型绿色荧光蛋白基因编码序列克隆到不含启动子的载体pLAFR6上,构建报告质粒pLZY。
4)Enhanced green fluorescent protein增强型绿色荧光蛋白
1.Construction of expression vectors of enhanced green fluorescent protein gene and human extracellular 1-3domain of vascular endothelial growth factor(VEGF) receptor KDR gene;增强型绿色荧光蛋白基因与人血管内皮生长因子受体KDR基因胞外1-3区域融合表达载体的构建
2.Cell-penetrating peptide PEP-1 mediated transmembrane delivery of enhanced green fluorescent protein in vivo of mouse;细胞穿透肽PEP-1介导增强型绿色荧光蛋白在小鼠体内跨膜转导
3.Cell-penetrating Peptide PEP-1-mediated Transduction of Enhanced Green Fluorescent Protein into Human Aortic Smooth Muscle Cells;PEP-1介导增强型绿色荧光蛋白转导入人主动脉平滑肌细胞
5)Enhanced green fluorescent protein (EGFP)增强型绿色荧光蛋白
1.Objective To construct a novel enhanced green fluorescent protein (EGFP) tagged insect-baculovirus transference system and prepare EGFP by Sf-9 cells.目的构建可表达增强型绿色荧光蛋白(EGFP)融合蛋白的昆虫杆状病毒转移载体,利用Sf-9细胞表达、制备EGFP。
2.Objective To construct a novel enhanced green fluorescent protein (EGFP) tagged Hela cell subline.目的构建增强型绿色荧光蛋白(EGFP)标记的Hela细胞系。
6)Enhanced green fluorescent protein C3增强型绿色荧光蛋白C3
延伸阅读

稀土绿色荧光粉分子式:CAS号:性质:目前用做投影电视绿色荧光体主要是钇铝镓石榴石体系[Y3(Al,Ga)5O12],如YAG:Tb(P53),Y(A1,Ga)G:Tb等,具有良好的温度猝灭特性和亮度线性关系。BaF2是制备单相立方YAG:Tb荧光粉良好的助熔剂,能提高磷光体的发光效率。用镓取代部分铝,代号为P53(Ga),其老化特性,亮度和饱和特性,γ系数等都得到改善。