专利名称：Prv－1基因及其应用的制作方法
婕癙RV-1暮校写撕械DNA懈DNA约些转细婕癙RV-1模硕牡目澹琍RV-1牡姆约PRV-1幕PRV-1牡目药铩
细症(Polycythaemia rubra vera)(erythraermia)也为Polycythaemia veraP-vera一侄血液学细细途藓细纬印貌源诳隆傻血前细突隆诘鹿细症姆为每10046恕疲貌18诘梅血苹平映13洗时洹
细症俅指希俅之头痛搔脾诙之一谐血血栓血压叩碌痛纾寄承┞撑 要实曳血斓白⊙篙∠ 芎细莼值撸鲁细血小症为一指嗟蹦Ｒ 谢呔些指辏ㄑ较钢 约细血胤血小症挚瞬鼙证确浴目前细症姆学全未知为坪细症展夭蹋司确欠浅要摹
耍目欠趾细症姆学颍峁┫该材吭
目通一只实郑没细症乇铮秸感诧。一为PRV-1(polycythaemia rubra vera)
频暮诠WO98/50552泄
耍一婕癙RV-1亩幔 习SEQ ID NO.1小亩堑双DNARNARNA员知U源T凇应为指15暮帷
暮
图1示耍婕坝ν 1示械亩幔际疚⑿∧ 帷Ｚ壁Ｎ⑿≈秆 0乇选25员没歉煤幕墓懿谋洹Ｔ毕ひ皇 氚蹦济蓖 没猓惨比笔 约突洹Ｒ 囟实施校SEQ ID NO.136-1346位幔琍RV-1谋一实施SEQ ID NO.136-1262位幔辉て盤RV-1牡幕螅亩也砂SEQ ID NO.139-1346位39-1262位39-1238位幔妒 硬凇一选实施前SEQ ID NO.199-1346位99-1262位99-1238位亩幔导卤PRV-1牡藕牡5末硬凇
亩也PRV-1一片危通片尉卸100幔∝ 00帷Ｆ 探耄盤CR诖拢片慰员屎细目摹通锍の 0-30幔诫长为15-50帷
PRV-1一源曰颍诮械谋佟拢要血偌胎斜铮，唷⑩、倩胁铩 细症校一乇血细蟹浅强毓铩
PRV-1牡图2示斜牡写诘牟碌始庸时去藕酆(---)挚示SEQ ID NO.2小耍一为洗木SEQ ID NO.2械模呔SEQ ID NO.2械缺藕牡亩(SEQID NO.222-437位)实施SEQ ID NO.21-409位22-409位1-401位22-401位(玫实幕)
堑学裕选腔模选N-腔摹然PRV-1牡陌Asn-46Asn-189Asn-382械一岽η 挪SEQ ID NO.2)牡片危N-摹片蔚某为50幔 00幔 50帷Ｒ皇凳┬Ｄ O-腔摹
员牵囟陌没玫实学浴式谋()也潜一帧没嵌缘学没懈影没员知选没
票PRV-1目一腔脂锚然应SEQ ID NO.2邪407-409陌稀GPI锚通细膜脂锚诺省牵目前未证实原通鄄斓紾PI拥牡也头诺校一为洹蹦壳靶睬芬惶 值芸胤式酶细膜呀猓疵 丧失耍浅艿PRV-1细膜虾桶帧式膜系模芏诟枚为雍萍酶要为为一樱式芄散细
员牵通些C-末税影斓笆断改つ概∝辨及票囟DNA澹琍RV-1幕一牡片巍些涌员突缺失
uPAR/Ly6谋濉Ｒ 转丝藕牛碳细训藕拧屑为PRV-1墓铮壁合钢⑦的瓜 墓铮┫改怪
逊PRV-1诮约细血细血胤血小症诙血症幕叩细喜铩
为芙PRV-1亩诜图夥叫 苑卮DNA胁DNA选匕一泄拥SEQ ID NO.1暮校PRV-1谋SEQ ID NO.136-1346位幔 9-1262位39-1238位帷NA也山SEQ ID NO.1一片巍
婕靶盤RV-1幕片蔚DNA澹济缸咀赶冈 说模细蟪Ω司亩牟腔摹选细芄诜腔牡剩式胃玫省细细Sf9细状转染铮 槎窩OS细CHO细HeLa细些缘摹使媒母细为细员细同腔模式也同学也杀浠毖∏仓肥瓜 谋腔鼙指玫实学浴
一姹炬及一票牡姆址校氡灸礑NA细斜铮篙 亩潜细诮省然细校拇驶细蟹然远慕浓圆式坠姆色遣凇状原实(妫琑.Springer Verlag1994)一拇椎姆一选校姆私腔亩慕浓/虼炕牟瑁 诒丫媳之前之诤一校牡腔纸疟诟选姆校N-谋缘胤一校SEQ ID NO.2Asn-46位Asn-189位Asn-382位一岜磺 亩谋
细幕PRV-1目虾细症聘眉
一瓶恰啤一谩濉盧NA樱PRV RNARNAPRV-1 RNA淇 5-AAAAGCAGAAAGAGATTACCAGCC-3(SEQID NO.3)指一械谋姆RNA应潞校5-GGCTGGTAATCTCTTTCTGCTTTT-3(SEQ IDNO.4)一RNA宀⑾钢⑾ -RNA通转染耄狙 氐细症细RNA谋使PRV-1 mRNA芊啥摹源朔式细纬魏PRV-1住
嘶婕跋钢⒛汾 PRV-1幕位确潭取
一诠细细系墓谴诤细症强指示一煞夭PRV-1目通叻屑狻Ｊ的苍分 浠疨RV-1钥强澹 强员潭液小知式斜牵梅同位兀荧蚍⒐猓干 确幕拧些浠痹敝Ｚ 细汀荼乇选ELISA浴
PRV-1目知姆式频隆投隆呛实模选使玫隆濉
愿玫实也票濉Ｚ壁 镁械浅晒模a)KVSDLPRQWTPKN(34-46)(SEQ ID NO.5)b)SAREKRDVQPPASQH(391-405)(SEQ ID NO.6)
隆通通PRV-1吆实()应时PRV-I一学支()稀隆咏知通缀痛票痛HarlowLane目实植幔狄崇。
猓琍RV-1侄隆虻タ隆也萍
一实施校PRV-1RT-PCR狻 为耍却通为细墓PRV-1细蟹RNA然RT锞阶糝T选蔷潞械(SEQ ID NO.7)
ATTAGGTTATGAGGTCAGAGGGAGGTT
址式PRV-1 RNA转DNA一DNA一PCR应知式循选铮
(SEQ ID NO.8)
GCAGAAAGAGATTACCAGCCACAGACGG
(SEQ ID NO.9)
GAATCGTGGGGGTAATAGAGTTAGCAGG
员芄使霉业实铩
RNA一始希私PRV-1也时PCR藕挪懦浴只械呋泻细症时恕PRV诮细胁铮 魏RT-PCR藕指示诤细症RT-PCR械选TaqMan眉要探搿 探选5-TTCTTGTTGAACCACACCAGACAAATCGG-3(SEQ ID NO.10)思PRV-1亩RT-PCR也潜一帧
撸也使印选Northern印虾细症址细蟹RNA然印Northern印PRV-1谋铩EQ ID NO.1cDNA慰探搿Ｏ冈恍合钢⒛ 时咏欧为只写时细喜斜铩Ｓ 指示细母未泻细症
没片谓Northern印咏片纬通100选炔300撸Northern印探母没同片慰通酶票片源cDNA双冢傻咏实420bp-831bpBamHI-PstI片危831bp-1900bpPstI-PstI片巍
冉mRNA一RT-PCR转录然cDNA一咏探DNA也杉PRV-1mRNA约PRV-1谋铩
拢聘貌为然越痰时诘疲使每PRV-1炜瑰，要每细猿煞纸稀
私一婕耙灰╋，药娓惩 猓琍RV-1目濉Ｎ狿RV-1诤细症泄铮鸽抗PRV-1哟时嗫 妗｀抗些细慕洗碳学细些细源朔式细症细
司兀逊PRV-1某血裕PRV-1艽碳某些血前细纬珊细洹-腔PRV-1展示一堋选亩N-腔PRV-1募展示踊缘片巍
一为一药铮耙┭ 母图猓琍RV-1幕学片巍PRV-1选腔PRV-1模选N-腔PRV-1幕学片巍婕耙恢 药铩
一婕癙RV-1学片为诨(ex vivo)拥应谩PRV-1幕学片乒循械全血细症全血细(血凸细值谋浠 亩懿全苹全碌贫血苹苹全械园细症血小症()突牖歼笛冈寂 症系统应酆症(SIRS)植苑应亩幕药愿址式琛ｈ方式凇凇皮隆膜凇诜皮途粘膜琛
亩也全血细症全血细诖拢目诨细斜PRV-1幕涔ζ 危纱炔要没品纱踊蟹细帽转染()使欠摺也钥姆斜亩峋 平细谋血浴
司鹊鼗诟浓时PRV-1亩细茫鄄斓絇RV-1椎实全止撕细细/细纬桑效应诟-茫弑怨血(CMLchronic myeloidleukemia)秃细症幕啤一源缘细幕学萍染懈诺悖当-没谢时一围使玫腔濉 一缺只曰氐母茫施酶-牟嘶腔一氐小婕耙恢 浓鹊血省
吮一婕摆盤RV-1牡途细乇为一细途婕懊兑灰╋。些枪约细症胤血小唷 维浴CML械陌血馨实
一潜亩帷 片渭员细途员植一实宀⒆臼的跋窹RV-1摹片渭员一浓时突效应同兀员植一澹 实陌细懿染械PRV-1突铩 也婕靶的对家 途乒约细症胤血小唷 维浴CML械陌血馨实
婕摆枷钢⒀低骋翟 校些约邪亩/虮痉亩暮/虮痉一挚濉Ｖ，约谢砂实施夥从︼。为液膜约咏液诙濉⑩反应牡液取约选诮PCR应Northern印Southern印Western印ELISARIA品应
实施诮捅实施1PRV募
实约
-诖哟血血细症呋玫血细
-血屑0.9NaCl械3暇牵(RT)20印
-啵ハ睬成并1800g10印
-去澹竿 .9NaCl小
-每拢35mlNaCl械细15ml Ficoll-Hypaque稀
-然1800gRT刹Ficoll-Hypaque系细60印
-纬细途屑恪
-屑去细10ml0.2NaCl30耄 0ml1.6NaCl
-细1800gRT10印
-然10ml PBS洗1危摹
-细砗 5-99鹊细
-帽准些细蟹RNA
-Northern印屑10mg RNAPRV-1谋铩EQ IDNO.1示cDNA为探搿
一实39细症吆29血品小诤细症蟹PRV-1探强咏品未鄄斓接 实施2PRV-1踊
芫13.5幕母取胎取胎危每染色胎细通囟细慕细汀獾枷感改承┭跋 细纬傻位CFU-E)耍约2胎CFU-E桑30-40细CFU-E伞
转录转染些CFU-E为耍为293-T陌装细系48小时前转染293-T细系呀胎细系转录募只榷转染293-T细些293-T细贸为pOSpKAT转染芨染胎细转录293-T细每pOSpKAT转染转录椎野转录一妫籔RV-1隆pOS宓悸踩鞠 时一椎转录293-T细转录诮细
栈转录转染293-T细通0.45m膜1巍为转染胎细些细牒寄顾迪赶Ｚ Polybren拇拢1800rpm202小时转染胎然(MethocultCell Systems)通魏桶猓ヅＱ，0.0001-0.4IU俸细(EPO)/ml图谆维(0.8)CFU-E要EPO纬血洹Ｗ晃固簧棺从 使液喾此可观察到一细欠纬血洹FU-E纬珊细洌貉 前细募洹
3螅纬傻血冉烁只铮 一实没屑谢铮 -3欠浅频亩眨械每一4直员取
1未转录转染细
2每pOS转染细
3梅血缘色荧獾 GFP)转染细
4pOS-PRV-1(+)转染细
1谐械3实玫慕每一执砑
实证PRV-1转染CFU-E雀侄CFU-E纬啥梅浅募(3)一PRV-1CFU-E印实施3PRV-1拥目
薪一实芯PRV-1欠一艿樱欠要细-细哟装细系293-TpOSpKAT转染蟛唤转录293-T细铣煽隆pOS械幕牡剩诒PRV-1强艿祝浔悔狡白跋赶 93-T小293-T细pOSpKAT转染纬转录细细目艿住pOS-PRV-1转染细牟转录CFU-E希全涂诩谆维校玫募
2PRV-1目裕每一执砑
一实校同煤PRV-1CFU-E雀侄细纬啥梅浅血洌籔RV-1强艿印实施4PRV-1细
实血细薪械摹诮血液循一小前细锌屎辖(谆维)血细血细一(脂EDTA为一挚血)取40ml芫血液15ml/hypaque血液1600rpm转幕40印一纸血液细要氐芏荻取暮螅懈细牡细血途屑唷Ｆ 屑啵琍BS(确液)洗印舜牡细约0.1血细
细诤-浓鹊3FCS(牛胎血)乇岣 IMDM)斜眨3FCS铮 欠PRV-1
IMDM械牡细7105细/ml芏燃Methocult(细)峁┮瞪恍 IMDM30FCS1BSA(牛血宓 匣掖2mlL-劝危3IU EPO(俸细)/ml1.0募谆维亍细14欤恍的赶 苑血洹Ｍ 105细100200血细
一芨卟PRV-1细系些细PRV-1谋洌阂恢含SEQ IDNO21-401位陌幔笔 02-437位陌帷 烁谋PRV-1野PRV-1通一脂锚细膜全细凇细系实施3械锏 PRV-1)魏转录293细
为私血血细未转染293细48小时IMDM斜眨谋PRV-1细(293-GPI-less-PRV-1)48小时斜铡殴些细纬血14确细(色)凸(色)血细目实馗危同貌同血液濉Ｒ 实械馗玫慕拢
实1
实2
实3
些菘缘贸一郏实施3懈呒PRV-1细谩实施5PRV-1N-腔
踊泻细症幕叻细帽准些细票取铩oehringer Mannheim峁┠-酶F去腔约小姆些取铩Ｏ肛 一曰液氲饺⌒ 5娲 樱之谩应液颉胺应液N-酶怼 每一37姹Ｂ挂筆AGE泳Western印省PRV-1每SEQ ID NO.5陌械实目猓聪窹RV-1状小为60-65kDaN-酶小40kDa证PRV-1於 於琋)腔摹
斜<110>学酆医院<120>PRV-1应<130>E980930<140>PCT/EP00/09594<141>2000-09-29<150>DE 199 47 010.3<151>1999-09-30<160>10<170>PADAT SequenzmodulVersion 1.0<210>1<211>1600<212>DNA<213>homo sapiens<220><223><400>1aaaagcagaa agagattacc agccacagac gggtcatgag cgcggtatta ctgctggccc 60tcctggggtt catcctccca ctgccaggag tgcaggcgct gctctgccag tttgggacag 120ttcagcatgt gtggaaggtg tccgacctgc cccggcaatg gacccctaag aacaccagct 180gcgacagcgg cttggggtgc caggacacgt tgatgctcat tgagagcgga ccccaagtga 240gcctggtgct ctccaagggc tgcacggagg ccaaggacca ggagccccgc gtcactgagc 300accggatggg ccccggcctc tccctgatct cctacacctt cgtgtgccgc caggaggact 360tctgcaacaa cctcgttaac tccctcccgc tttgggcccc acagccccca gcagacccag 420gatccttgag gtgcccagtc tgcttgtcta tggaaggctg tctggagggg acaacagaag 480agatctgccc caaggggacc acacactgtt atgatggcct cctcaggctc aggggaggag 540gcatcttctc caatctgaga gtccagggat gcatgcccca gccaggttgc aacctgctca 600atgggacaca ggaaattggg cccgtgggta tgactgagaa ctgcaatagg aaagattttc 660tgacctgtca tcgggggacc accattatga cacacggaaa cttggctcaa gaacccactg 720attggaccac atcgaatacc gagatgtgcg aggtggggca ggtgtgtcag gagacgctgc 780tgctcataga tgtaggactc acatcaaccc tggtggggac aaaaggctgc agcactgttg 840gggctcaaaa ttcccagaag accaccatcc actcagcccc tcctggggtg cttgtggcct 900cctataccca cttctgctcc tcggacctgt gcaatagtgc cagcagcagc agcgttctgc 960tgaactccct ccctcctcaa gctgcccctg tcccaggaga ccggcagtgt cctacctgtg 1020tgcagcccct tggaacctgt tcaagtggct ccccccgaat gacctgcccc aggggcgcca 1080ctcattgtta tgatgggtac attcatctct caggaggtgg gctgtccacc aaaatgagca 1140ttcagggctg cgtggcccaa ccttccagct tcttgttgaa ccacaccaga caaatcggga 1200tcttctctgc gcgtgagaag cgtgatgtgc agcctcctgc ctctcagcat gagggaggtg 1260gggctgaggg cctggagtct ctcacttggg gggtggggct ggcactggcc ccagcgctgt 1320ggtggggagt ggtttgccct tcctgctaac tctattaccc ccacgattct tcaccgctgc 1380tgaccaccca cactcaacct ccctctgacc tcataaccta atggccttgg acaccagatt 1440ctttcccatt ctgtccatga atcatcttcc ccacacacaa tcattcatat ctactcacct 1500aacagcaaca ctggggagag cctggagcat ccggacttgc cctatgggag aggggacgct 1560ggaggagtgg ctgcatgtat ctgataatac agaccctgtc 1600<210>2<211>437<212>PRT<213>homo sapiens<400>2Met Ser Ala Val Leu Leu Leu Ala Leu Leu Gly Phe Ile Leu Pro Leu 1 5 10 15Pro Gly Val Gln Ala Leu Leu Cys Gln Phe Gly Thr Val Gln His Val
20 25 30Trp Lys Val Ser Asp Leu Pro Arg Gln Trp Thr Pro Lys Asn Thr Ser
35 40 45Cys Asp Ser Gly Leu Gly Cys Gln Asp Thr Leu Met Leu Ile Glu Ser
50 55 60Gly Pro Gln Val Ser Leu Val Leu Ser Lys Gly Cys Thr Glu Ala Lys 65 70 75 80Asp Gln Glu Pro Arg Val Thr Glu His Arg Met Gly Pro Gly Leu Ser
85 90 95Leu Ile Ser Tyr Thr Phe Val Cys Arg Gln Glu Asp Phe Cys Asn Asn
100 105 110Leu Val Asn Ser Leu Pro Leu Trp Ala Pro Gln Pro Pro Ala Asp Pro
115 120 125Gly Ser Leu Arg Cys Pro Val Cys Leu Ser Met Glu Gly Cys Leu Glu
130135 140Gly Thr Thr Glu Glu Ile Cys Pro Lys Gly Thr Thr His Cys Tyr Asp145 150 155 160Gly Leu Leu Arg Leu Arg Gly Gly Gly Ile Phe Ser Asn Leu Arg Val
165 170 175Gln Gly Cys Met Pro Gln Pro Gly Cys Asn Leu Leu Asn Gly Thr Gln
180 185 190Glu Ile Gly Pro Val Gly Met Thr Glu Asn Cys Asn Arg Lys Asp Phe
195 200 205Leu Thr Cys His Arg Gly Thr Thr Ile Met Thr His Gly Asn Leu Ala
210 215 220Gln Glu Pro Thr Asp Trp Thr Thr Ser Asn Thr Glu Met Cys Glu Val225 230 235 240Gly Gln Val Cys Gln Glu Thr Leu Leu Leu Ile Asp Val Gly Leu Thr
245 250 255Ser Thr Leu Val Gly Thr Lys Gly Cys Ser Thr Val Gly Ala Gln Asn
260 265 270Ser Gln Lys Thr Thr Ile His Ser Ala Pro Pro Gly Val Leu Val Ala
275 280 285Ser Tyr Thr His Phe Cys Ser Ser Asp Leu Cys Asn Ser Ala Ser Ser
290 295 300Ser Ser Val Leu Leu Asn Ser Leu Pro Pro Gln Ala Ala Pro Val Pro305 310 315 320Gly Asp Arg Gln Cys Pro Thr Cys Val Gln Pro Leu Gly Thr Cys Ser
325 330 335Ser Gly Ser Pro Arg Met Thr Cys Pro Arg Gly Ala Thr His Cys Tyr
340 345 350Asp Gly Tyr Ile His Leu Ser Gly Gly Gly Leu Ser Thr Lys Met Ser
355 360 365Ile Gln Gly Cys Val Ala Gln Pro Ser Ser Phe Leu Leu Asn His Thr
370 375 380Arg Gln Ile Gly Ile Phe Ser Ala Arg Glu Lys Arg Asp Val Gln Pro385 390 395 400Pro Ala Ser Gln His Glu Gly Gly Gly Ala Glu Gly Leu Glu Ser Leu
405 410 415Thr Trp Gly Val Gly Leu Ala Leu Ala Pro Ala Leu Trp Trp Gly Val
420 425 430Val Cys Pro Ser Cys
435<210>3<211>24<212>RNA<213>斯<220><223>PRv-1械5末<400>3aaaagcagaa agagattacc agcc 24<210>4<211>24<212>RNA<213>斯<220><223><400>4ggctggtaat ctctttctgc tttt 24<210>5<211>13<212>PRT<213>斯<220><223>PRV-1陌34-46<400>5Lys Val Ser Asp Leu Pro Arg Gln Trp Thr Pro Lys Asn 15 10<210>6<211>15<212>PRT<213>斯<220><223>PRV-1陌391-405<400>6Ser Ala Arg Glu Lys Arg Asp Val Gln Pro Pro Ala Ser Gln His 15 10 15<210>7<211>27<212>DNA<213>斯<220><223>RT<400>7attaggttat gaggtcagag ggaggtt 27<210>8<211>28<212>DNA<213>斯<220><223><400>8gcagaaagag attaccagcc acagacgg28<210>9<211>28<212>DNA<213>斯<220><223><400>9gaatcgtggg ggtaatagag ttagcagg 28<210>10<211>29<212>DNA<213>斯<220><223>探<400>10ttcttgttga accacaccag acaaatcgg29
权利要求
1习之一N-腔模
SEQ ID NO.2陌1-437
SEQ ID NO.2陌1-409
SEQ ID NO.2陌1-401
SEQ ID NO.2陌22-437
SEQ ID NO.2陌22-409
SEQ ID NO.2陌22-401
浜 0片巍
2习之一亩模
SEQ ID NO.2陌1-409
SEQ ID NO.2陌1-401
SEQ ID NO.2陌22-409
SEQ ID NO.2陌22-401
3一挚权要1牡目濉
4权要3目澹恢德 濉
5一旨细症姆一叻校PRV-1一权要34目宸 应
6权要5姆诳一权要34亩隆堑隆濉
7一坪细症药铮惩 渭猓ㄒ 4目濉
8一职权要1幕腔习之一亩牡药铮
SEQ ID NO.2陌1-437
SEQ ID NO.2陌1-409
SEQ ID NO.2陌1-401
SEQ ID NO.2陌22-437
SEQ ID NO.2陌22-409
SEQ ID NO.2陌22-401
一药学峡山艿母渭
9一职药铮 之一
SEQ ID NO.1暮1-1600
SEQ ID NO.1暮36-1346
SEQ ID NO.1暮36-1262
SEQ ID NO.1暮36-1238
SEQ ID NO.1暮39-1346
SEQ ID NO.1暮39-1262
SEQ ID NO.1暮39-1238
SEQ ID NO.1暮99-1346
SEQ ID NO.1暮99-1262
SEQ ID NO.1暮99-1238
一药学峡山艿母渭
10权要1亩幕习之一亩幕片位员为拥应茫为
SEQ ID NO.2陌1-437
SEQ ID NO.2陌1-409
SEQ ID NO.2陌1-401
SEQ ID NO.2陌22-437
SEQ ID NO.2陌22-409
SEQ ID NO.2陌22-401
11权要1亩幕习之一亩幕片位员乒循全血细症全血细药械应茫为
SEQ ID NO.2陌1-437
SEQ ID NO.2陌1-409
SEQ ID NO.2陌1-401
SEQ ID NO.2陌22-437
SEQ ID NO.2陌22-409
SEQ ID NO.2陌22-401
12习之一亩片位乒循全血细症全血细药械应茫为
SEQ ID NO.1暮1-1600
SEQ ID NO.1暮36-1346
SEQ ID NO.1暮36-1262
SEQ ID NO.1暮36-1238
SEQ ID NO.1暮39-1346
SEQ ID NO.1暮39-1262
SEQ ID NO.1暮39-1238
SEQ ID NO.1暮99-1346
SEQ ID NO.1暮99-1262
SEQ ID NO.1暮99-1238
13权要1亩幕习之一亩幕片位员坪/源细/饨ㄏ赶敌涤γＮ
SEQ ID NO.2陌1-437
SEQ ID NO.2陌1-409
SEQ ID NO.2陌1-401
SEQ ID NO.2陌22-437
SEQ ID NO.2陌22-409
SEQ ID NO.2陌22-401
14权要1亩幕习之一亩幕片位员细械应茫为
SEQ ID NO.2陌1-437
SEQ ID NO.2陌1-409
SEQ ID NO.2陌1-401
SEQ ID NO.2陌22-437
SEQ ID NO.2陌22-409
SEQ ID NO.2陌22-401
15权要14应茫诙为一细
16权要1亩幕习之一亩幕片位员药械应茫为
SEQ ID NO.2陌1-437
SEQ ID NO.2陌1-409
SEQ ID NO.2陌1-401
SEQ ID NO.2陌22-437
SEQ ID NO.2陌22-409
SEQ ID NO.2陌22-401
17权要16应茫选椋涸枷钢⒇费∴，维裕CML械陌血馨实
18一只习之一亩片位细械应茫为
SEQ ID NO.1暮1-1600
SEQ ID NO.1暮36-1346
SEQ ID NO.1暮36-1262
SEQ ID NO.1暮36-1238
SEQ ID NO.1暮39-1346
SEQ ID NO.1暮39-1262
SEQ ID NO.1暮39-1238
SEQ ID NO.1暮99-1346
SEQ ID NO.1暮99-1262
SEQ ID NO.1暮99-1238
19一只习之一亩片位药械应茫为
SEQ ID NO.1暮1-1600
SEQ ID NO.1暮36-1346
SEQ ID NO.1暮36-1262
SEQ ID NO.1暮36-1238
SEQ ID NO.1暮39-1346
SEQ ID NO.1暮39-1262
SEQ ID NO.1暮39-1238
SEQ ID NO.1暮99-1346
SEQ ID NO.1暮99-1262
SEQ ID NO.1暮99-1238
20权要19应茫选椋涸枷钢⒇费∴，维浴CML械陌血馨实
21一诩细症血系统业约校约邪一只习之一亩幔
SEQ ID NO.1暮1-1600
SEQ ID NO.1暮36-1346
SEQ ID NO.1暮36-1262
SEQ ID NO.1暮36-1238
SEQ ID NO.1暮39-1346
SEQ ID NO.1暮39-1262
SEQ ID NO.1暮39-1238
SEQ ID NO.1暮99-1346
SEQ ID NO.1暮99-1262
SEQ ID NO.1暮99-1238
片位澹
一权要1幕习之一亩模
SEQ ID NO.2陌1-409
SEQ ID NO.2陌1-401
SEQ ID NO.2陌22-409
SEQ ID NO.2陌22-401
片位员澹
一权要34目濉
22权要21募PRV-1椎约校一ELISA约小
23权要21募PRV-1椎约校一职攵≧T-PCR约小
24权要21募PRV-1椎约校一Northern印咏约小
全文摘要
本发明描述了一种编码PRV－1蛋白的核苷酸序列,其基本上包含序列SEQ ID NO.1的核苷酸序列。本发明还涉及检测该基因及由该基因编码的mRNA和多肽的方法。
文档编号A61P9/00GK1377408SQ00813580
公开日2002年10月30日 申请日期2000年9月29日 优先权日1999年9月30日
发明者海克·帕尔 申请人:弗赖堡大学综合医院