1)RT-PCRRT-PCR法
1.The Detection of Norwalk Virus in Food by RT-PCR;用RT-PCR法检测食品中诺沃克样病毒
2.Clinical significance of detecting plasma HIV RNA concentration by RT-PCR;RT-PCR法检测血浆HIV RNA浓度的临床意义
3.Method To gather 500 blood serum samples of patients (diagnosis undetermined, DU), apply restriction enzyme analysis,Elisa, RT-PCR analysis to detect samples three times, and contrast the results of each method.方法收集500例疑似病毒性心肌炎的患者血清,分别采用限制性内切酶分析法、ELISA法、RT-PCR法3种检测方法进行检测,每种方法在相同条件下连续重复3次,然后将其结果进行比较。
英文短句/例句
1.Expression of HCV in Hepatocellular Carcinoma Detected by PCR Technique应用RT-PCR法检测肝细胞癌的HCV表达
2.Detection of Lymph-Micrometastisis of Pationts with NSCLC by Using Real-Time RT-PCR;实时RT-PCR法检测非小细胞肺癌淋巴微转移
3.Detection of Multidrug-Resistance mdr-1 Gene Expression Using Fluorescent Quantitative RT-PCR;应用荧光定量RT-PCR法检测mdr-1基因的表达
4.Diagnosis Establishment Formulation of RT-PCR、Fluorescence Quantitative PCR and Mutiplex RT-PCR Detection for Japanese Encephalitis Virus;猪乙脑病毒RT-PCR、荧光定量PCR及多重RT-PCR诊断方法建立
5.Establishment of Nested RT-PCR and Real-Time Fluorescence RT-PCR Methods to Detect West Nile Virus;西尼罗病毒套式RT-PCR和实时荧光RT-PCR检测方法的建立
6.Establishment of Rapid Diagnostic Methods for Detection of West Nile Virus by Nest RT-PCR and TaqMan Real-time RT-PCR;西尼罗病毒巢式RT-PCR和荧光定量RT-PCR两种检测方法的建立
7.Detection of Bean pod mottle virus in soybean by one step assay of RT-PCR and real-time fluorescent RT-PCRRT-PCR和实时荧光RT-PCR一步法检测大豆中菜豆荚斑驳病毒
8.A QUICK AND MORE SENSITIVE METHOD TO IDENTIFY PORK IN PROCESSED AND UNPROCESSED FOOD BY PCR AND REALTIME-PCR METHOD高效快速检测食物中猪成分的PCR和RT-PCR方法
9.One-step RT-PCR for detection of SARS associated coronavirus一步法RT-PCR检测SARS冠状病毒技术研究
10.Detecting HCV RNA by one-step short-product RT-PCR一步短片段RT-PCR方法检测hcv RNA
11.Quantitative Determination of IFN-γ mRNA by the Competitive RT-PCR Method竞争RT-PCR定量测定IFN-γmrna方法的建立
12.Establishment of the Diagnostic Method for the RT-PCR and ELISA of Vesicular Stomatitis;水泡性口炎的RT-PCR及ELISA诊断方法建立
13.Development of a Real-time RT-PCR Assay for the Detection of Nipah Virus;尼帕病毒荧光RT-PCR检测方法的建立
14.A Study on the one-step RT-PCR for detection of potato Viruses;马铃薯病毒一步法RT-PCR诊断研究
15.DETECTION OF NOROVIRUSES IN SHELLFISH BY RT-PCR;贝类产品中诺沃克病毒RT-PCR检测方法
16.The Establishment of RT-PCR Diagnostic Method for Swine Influenza猪流感RT-PCR诊断方法的建立
17.A RT-PCR Method Suitable for MicroRNA Identification of Soybean一种适合大豆MicroRNA鉴定的RT-PCR方法
18.Establishment of RT-PCR method for rapid detection of canine distemper犬瘟热RT-PCR快速诊断方法的建立
相关短句/例句
RT-PCR methodRT-PCR法
1.We use Immunohistochemistry SP method to examine the expression of P73 and MDM2 protein, and RT-PCR method was adopted to examine the expression of P73 and MDM2 gene.方法采用免疫组化SP法对40例子宫内膜样腺癌、40例非典型增生内膜组织、40例正常子宫内膜组织共120例石蜡包埋组织进行P73、MDM2基因表达蛋白的检测,并应用RT-PCR法对40例子宫内膜样腺癌中P73、MDM2基因的表达进行检测,应用SPSSl3。
3)Real-time PCR assay实时RT-PCR法
4)End-point RT-PCR终点法RT-PCR
5)One-step RT-PCR一步法RT-PCR
1.Detection of Citrus psorosis virus by one-step RT-PCR and its annual distribution in different parts of Dweet tangor;运用一步法RT-PCR检测柑橘鳞皮病毒及其在橘橙不同部位中的全年分布
2.Research and Application of One-step RT-PCR Kit in the Detection of Tobacco Ringspot virus;一步法RT-PCR检测烟草环斑病毒试剂盒的研制与应用
3.Development of a one-step RT-PCR assay for detection of A subtype avian influenza virus;禽流感病毒一步法RT-PCR检测方法的建立
6)one step RT-PCR一步法RT-PCR
1.E2 gene was amplified by one step RT-PCR from total RNA,which had been extracted from tissues of pigs doubting infected by classical swine fever virus and PK-15 infected by CSFV F114.自疑似猪瘟病料及接猪瘟F114毒的PK-15细胞中提取总RNA,经一步法RT-PCR扩增E2基因。
2.For detection of BPMV in imported soybean,the specific primers and Taqman probe were designed based on the coding gene of BPMV coat protein and one step RT-PCR and one step real-time fluorescent RT-PCR methods were developed.针对进口大豆的菜豆荚斑驳病毒(Bean pod mottle virus,BPMV)检测,建立了一步法RT-PCR和一步法实时荧光RT-PCR检测方法。
延伸阅读
［国外糖业技术］废蜜糖份连续回收(RT法)废蜜中糖份回收，排出废液浓缩后作饲料，是糖厂废蜜无污染利用的可取方案之一。RT废蜜糖份连续回收法，与旧斯蒂芬法对比，其优点是：生产连续，设备简单，用钢材少，糖份回收率高，操作简便。在法国OrignySainte-Benoite糖厂采用此法的全自动化废蜜糖份回收车间，只要一人管理。一、工艺条件和设备的特点：1、废蜜糖份回收工艺成败的关键是石灰粉的质量，要求如下：(1)、选用粒度偏小而均匀，纯度高、比重较轻的石灰石，保证石灰石锻烧分解完全又不过烧。(2)、先用锤式粉碎机将石灰石由90～130毫米粉碎到10～13毫米，再进一步用球磨机粉碎到74微米以下，大于74微米的石灰粉不得超过10%。(3)、选用经过冷却而又非常新鲜的生石灰。2、为保持废蜜锤度、温度稳定，糖厂排出的废蜜先送入贮罐，再从贮罐取用；废蜜在稀释桶2中，稀释至含糖12%；进稀释桶的废蜜和水先经计量后再入桶；充分利用蔗糖钙的洗水稀释。3、为使石灰粉和稀释废蜜很快均匀混合，生成蔗糖钙，并促使蔗糖钙粒子不断长大，采取以下措施：(1)、每吨石灰粉加300克甘油，以增加其流动性。(2)、加大循环量(约10倍)，通过泵6从反应槽3中部取出反应液，返回到进蜜端槽全长的1/6处，在循环流送过程中，使其通过溢流板溢流至流槽16中，在流槽中形成很薄的液层，石灰粉经称计量后通过分布器15均匀的撒在此薄液层上，这样通过槽内搅拌器和泵6的搅拌，很快混合均匀。加石灰粉量对蜜中糖约为1～1.3比1，流槽宽度约800毫米；石灰粉和稀释废蜜在反应槽中停留时间约20～40分钟，反应温度8～IO℃，反应槽搅拌器转速约15转/分。(3)反应槽采用U形底的卧槽，避免出现死角。(4)反应液用泵4从出料端取出，通过冷却器5(Alva-Iava型)冷却到8℃后，从加料端重返反应槽，循环量约为3倍，这样有利于蔗糖钙粒子的不断长大。冷却器5采用冷冻机冷却。4、反应后的蔗糖钙液，在沉淀器7中停留约5分钟，反应不完全的石灰粒子可很快沉降出来，被重新送往废蜜稀释桶，以节约用灰量。5、采用高效率橡胶水平带式真空吸滤机9(示意图)，可保证滤出之蔗糖钙层厚度均匀，不出裂纹，洗水良好，所得蔗糖钙经两段洗水，洗水量约为每吨蜜2.5立方米；所需带式过滤机的有效过滤面积约为日处理l0吨废蜜1平方米，真空度400mmHg柱。6、冷滤液通过泵10经加热器12加热到85℃，得热蔗糖钙沉淀，和冷蔗糖钙一齐过滤收回。7、经洗涤后的蔗糖钙在槽17中稀释后通过泵11送往车间供预灰、主加灰，稀释加水量约为每吨废蜜2立方米水。8、从桶13排出的废液，锤度约为10Bx,经瓦斯饱充过滤后，滤液用多效蒸发罐浓缩到锤度65-70Bx，供作饲料。在甜菜糖厂，可以与干粕混合后造粒。废液成份：干固物：68～70％；总灰份：20.7～23.5%；不溶灰份：微量；总粗蛋白：20.7～31.1%；可消化蛋白：18.5～27.6%；非氮抽出物：14.5～27.6%；还原糖：1.4%；总氮：3.9～4.4%；pH：10.5～11.5。9．废蜜糖份回收车间的换蜜量约为废蜜总处理垃的1/4；回收过程糖份损失约7%(对使用量．下同)，石灰损失约10%；每吨甜菜(折算)耗汽7.5公斤。附：流程图
