电击转化,electroporation英语短句,例句大全

电击转化,electroporation
1)electroporation电击转化
1.Results Optimized electroporation protocol that yielded highly effici.方法考察质粒经电击转化法转化根癌农杆菌 (A grobacteriumtumefaciensLBA440 4)的影响因素 ,并优化其转化条件 ;研究采用三亲杂交方法 ,将带有vgb基因簇的重组质粒 pYG3 0 8导入A 。
2.Using vector pBC-Hygro,the fungus was transformed to be hygromycin B-resistant,by conventional transformation as well as electroporation based on protoplast,electroporation based on germinated conidia,and restriction enzyme-mediated integration(REMI).以潮霉素作为抗性筛选标记,pBC-Hygro作为转化载体,用基于原生质体的传统转化和电击转化、基于萌发孢子的电击转化以及REMI技术转化红曲霉。
3.The DNA fragments were cloned into retroviral vector with EGFP marker and the ligation products were proceeded to electroporation in batch subsequently under optimal reaction condition and experiment parameter.方法:体外合成编码随机12肽的DNA片段;在最优化的实验参数和反应条件下将DNA片段克隆入带有EGFP标记的逆转录病毒载体后分批次电击转化大肠杆菌,合并转化所得菌液即为可表达随机12肽库的逆转录病毒原始载体库;半固体扩增法扩增该原始载体库,提取质粒并转染GP2-293包装细胞,在EGFP表达最强的时间点收集细胞培养上清,即为可表达随机12肽库的逆转录病毒库。

英文短句/例句

1.Construction and Electroporation of luxAB Expression Vector of Spirulina Platensis钝顶螺旋藻luxAB表达载体的构建及电击转化研究
2.Studies on Systems of High Efficiency Regeneration of Wheat Plants from Immature Embryos and Exogenous DNA Transfer by Electroporation;小麦幼胚高频再生及外源DNA电击转化体系的研究
3.Studies on the Induction of Calli from Isolated Microspores and the Exogenous DNA Transfer by Electroporation of the Semi-protoplasts of Wheat;小麦游离小孢子愈伤组织诱导及半原生质体外源DNA电击转化的研究
4.solid dielectric breakdown due to treeing固体电介质树枝化击穿
5.Click here to go to your e-mail inbox单击此处转到您的电子邮件收件箱
6.Did you watch the boxing match on TV?你有看电视转播的拳击赛吗？
7.Study on Electrotransformation of vitro DNA into Pseudomonas putida外源质粒电击转入Pseudomonas putida的条件研究
8.Operating characteristic of rotary piezo impact drive mechanism旋转型压电惯性冲击马达的工作特性
9.About the Tendency of Traditional Socialization under the Impact of Networks;网络化冲击下的传统社会化转型趋向
10.A study on antmortem and postmortem changes of sarcomere length of skeletal muscle in death from electrocution in rabbits;生前电击与死后电击骨骼肌肌小节的长度变化
11.Research of an Impact Rotary Piezo Motor Based on Torsional Actuator with Helical Electrodes基于螺旋电极扭转驱动器的冲击式旋转压电马达
12.Impact Between and Restructure of Internet Culture and TV Culture;互联网文化与电视文化的碰击与重构
13.The electrons must then be deflected to hit the correct pixels.（在新方法中）电子必须偏转以击中正确的像素。
14.Click the "next" button below to set up your call forwarding rules.单击下面的“下一步”按钮设置您的电话转接规则。
15.Research on Rotated Actuator Driven by Piezoelectric Inertial Impact Using Asymmetrical Gripper;非对称夹持式压电惯性冲击旋转驱动器的研究
16.The Bump of the Development of Networks and a Study on the Changing Pattern of the Socialization of Youngsters;网络化的冲击与青少年社会化的转型研究
17.The electric energy can change to light energy.电能可以转化为光能。
18.The Impact of EDP on General Counting and Auditing会计电算化对传统会计及审计的冲击