定点诱变,site-directed mutagenesis英语短句,例句大全

定点诱变,site-directed mutagenesis
1)site-directed mutagenesis定点诱变
1.PCR-based site-directed mutagenesis and recombinant expression plasmid construction of a SCN5A mutation (K317N) identified in a Chinese family with Brugada syndrome;中国人Brugada综合征SCN5A基因突变K317N的定点诱变及其载体构建
2.An improved PCR-based megaprimer method for site-directed mutagenesis;一种改进的大引物PCR定点诱变方法
3.It also in-troduce sorne active site amino acids, three-dimensional structure of xylanases and site-directed mutagenesis to xylanases.本文详细叙述了木聚糖酶分子的催化区域、纤维素结合区域、木聚糖结合区域、连接序列与重复序列的结构和功能方面的研究进展，并介绍了木聚糖酶分子中常见的活性位点氨基酸以及该酶的定点诱变和三级结构。

英文短句/例句

1.Site-directed mutagenesis of single base in vitro using circular site-directed mutagenesis technology环状定点诱变技术在单碱基定点诱变中的应用
2.Study on Site-directed Mutagenesis of Taq DNA Polymerase and Its Fidelity;Taq DNA聚合酶的定点诱变及保真度研究
3.Study on Improvement and Site-directed Mutagenesis of Antibacterial Peptide Protegrin抗菌肽Protegrin基因的定点诱变改良研究
4.Using Site-Directed Mutagenesis Techniques to Improve Xylanase s Thermostability;利用定点诱变提高木聚糖酶热稳定性的研究
5.Site-Directed Mutagenesis and Kinetics Analysis of Glyphosate N-acetyltransferase;草甘膦N-乙酰转移酶的定点诱变及动力学分析
6.Site-Directed Mutagenesis and Fouctional Studies in Cell Biology of Human PPPDE1 Gene人PPPDE1基因的定点诱变及其细胞生物学功能研究
7.Construction of Eukaryotic Expression Vector with a Site-directed Mutation of Porcine MSTN Propeptide Gene and Its Expression in C2C12 Cells猪MSTN前肽定点诱变载体在C2C12细胞中的表达
8.Charactering and Site-Directed-Mutagenesis of the S-Adenosylmethionine Synthetase Gene from Bacillus;芽孢杆菌属S-腺苷甲硫氨酸合成酶基因的活性鉴定与定点诱变
9.Cloning, Site-directed Mutagenesis of the Laccase Gene from Neurospora Crassa and Its Expression in Pichia Pastoris;粗糙脉孢菌漆酶基因的克隆、定点诱变及其在毕赤酵母中的表达
10.Construction of Site-Directed Mutagenic Plasmid Vector of Porcine Myostatin Propeptide Gene and Preliminary Functional Analysis猪肌肉生长抑素前肽基因定点诱变载体的构建和功能初步检测
11.Site-directed Mutagenesis on the Lipase Gene of Penicillium expansum FS1884 and the Activity Analysis of the Mutant扩展青霉FS1884脂肪酶基因的定点诱变及其活性分析
12.Cloning, Site-specific Mutation and Functional Analysis of Chemical-inducible Promoter;化学诱导启动子的克隆、定点突变及功能分析
13.The Mutantion of Wheat Induced by EMS and Identification and Screening of Mutantion;EMS诱导小麦突变及突变的鉴定和筛选
14.The Inducing of Polyploidy of Asparagus Officinalis L.and the Molecular Identification of Radiated Mutation;石刁柏多倍体诱导与辐射诱变的分子鉴定
15.Neither threats nor promises could change his inflexible determination.威胁与利诱均不能改变他坚定的决心。
16.Identification of Wheat M_2 Mutantions Induced by EMS;EMS诱导小麦M_2代突变的鉴定
17.Selection of Salt-Tolerant Mutants from Ethyl Methane Sulfonate Mutagenized Kiwifruit Embryonic CalliEMS定向诱导猕猴桃耐盐突变体筛选
18.The Gene Expression and the Function of the Mutants HIF-1α in Vivo不同位点诱变体低氧诱导因子1α的动物在体表达比较

相关短句/例句

site directed mutagenesis定点诱变
1.Using this as a template, prepared the two mutants (R778L, H1069Q) with USE site directed mutagenesis technique.【方法】采用RT-PCR和TOPO-TA克隆技术,克隆了全长WDcDNA,并以此为膜板应用USE定点诱变技术(特异性位点抹除技术)构建了778位(CGG→CTG)、1069位(GTG→GTC)两个突变体。
2.To locate the possible reactive site residues Lys 44 , Arg 76 and Arg 87 of APIB predicted according to the sequence comparison with other proteinase inhibitors, the above residues were substituted with Pro by site directed mutagenesis respectively, and the mutated genes were expressed in the yeast secretion system.为了找到它们的活性中心 ,利用定点诱变的方法将APIB中根据与其他抑制剂家族的序列比较所推断的可能的活性中心残基 :Lys44、Arg76和Arg87分别用Pro替代 ,所得到的突变基因分别在酵母分泌体系中得到了表达。
3.In order to improve the affinity of anti CD3 ScFv, one site directed mutagenesis method using "megaprimer" PCR was designed.利用巨型引物PCR定点诱变方法 ,设计并化学合成出两组含多个突变位点的简并引物 ,在第一轮PCR中使用简并引物分别扩增出含突变碱基的两条特异性的DNA片段 ,即巨型引物 ,将其经琼脂糖凝胶电泳分离纯化后 ,作为 3′和 5′的两端引物应用于第二轮PCR反应中。
3)site-directed mutation定点诱变
4)PCR site_directed mutagenesisPCR定点诱变
5)Site-directed mutagenesis PCR定点诱变PCR
1.Methods:Site-directed mutagenesis PCR was conducted to eliminate four phosphorylation sites of TAP26 respectively,then four mutant plasmids(TAP26(S48→A48),TAP26(S66→A66),TAP26(T167S168→V167A168),TAP26(T219→V219)) were produced;co-transfection study and luciferase activity measurement were used to observe the effect of wild TAP26 and four mutant plasmids on SP-C gene expression promotor.方法:采用定点诱变PCR技术产生TAP26的4个磷酸化位点突变体TAP26(S48→A48)、TAP26(S66→A66)、TAP26(T167S168→V167A168)、TAP26(T219→V219);通过体外细胞共转染后荧光素酶报告基因活性值检测技术研究野生型TAP26及其4个突变体质粒对SP-C基因启动子表达活性的影响。
6)Site-chosen mutation定点定位诱变