体外转录,in vitro transcription
1)in vitro transcription体外转录
1.In vitro transcription of eIF4E in laryngeal carcinoma;喉癌eIF4E基因的体外转录
2.Methods Transcription initiation at promoters of five genes selected from the two groups based on the transcriptome analysis was studied by means of in vitro transcription system.目的研究转录调控蛋白PrfA对两组新近发现的单核细胞增生李斯特菌基因的体外转录作用。
3.Objective To synthesize highly pure EBV-LMP1 carboxy region RNA by in vitro transcription and select its targeted active DNAzyme.目的探讨用体外转录方法制备EBV-LMP1C端RNA并用该RNA对其靶脱氧核酶进行初步筛选。
英文短句/例句

1.Amplification and Cloning of Full-length JEV cDNA and Preparation of Infectious Transcripts by in Vitro Transcription;乙脑病毒全长cDNA的扩增、克隆及体外转录制备感染性转录体的研究
2.The Preparation of Transcript in Vitro and Transfection to Giardia Canis Free from Giardiavirus;犬贾第虫病毒感染性体外转录体的制备及转染试验
3.Construction of Chimeric HCV cDNAs and Characterization of Their RNA Transcripts Infectivity in Vitro;嵌合型重组HCV cDNA的构建及体外转录RNA的感染特性研究
4.hTERT Core Promoter Modified by E-boxes Element and Transcriptional Activity Assay in vitroE-box元件修饰端粒酶核心启动子序列及体外转录活性分析(英文)
5.Construction of Full-length HCV cDNA with Cell Culture-adaptive Mutations and Preparation of Infectious RNA by in Vitro Transcription;HCV全长cDNA细胞培养适应性突变体的构建及其体外转录制备感染性RNA的研究
6.DNA Binding Characteristics and Inhibition of Transcription in Vitro of Novel Actinomycin D Analogs;抗癌药物放线菌素D新类似物DNA结合活性及体外转录抑制活性的研究
7.Comparative Transcriptome Analysis of Vibrio Parahaemolyticus in Response to Various Environmental Stresses副溶血性弧菌的体外比较转录组学研究
8.Research of Effect Resistin Gene Transfection on the Expression of Transcription Factors Sp1 and PPARγ mRNA in Vitro;抵抗素基因转染对转录因子Sp1及PPARγ mRNA表达影响的体外研究
9.Construction and Preliminary Application of Retroviral Vector which Carries EGFP GenepLXSN-EGFP逆转录病毒载体的构建及体内外表达的研究
10.The Significance of Transcription and Translation in the iRNA Mediated LAI Reaction in Vitro免疫核糖核酸体外介导白细胞粘附抑制反应中转录和翻译的意义
11.A Study of the in Vitro Interaction between the Multifunctional Transcriptional Regulator CTCF and Fragment of Vigilin;多功能转录因子CTCF与Vigilin片段体外相互作用的初步研究
12.In Vitro Molecular Evolution for DNA Binding Domain of Transcription Factor NtERF2;转录因子NtERF2 DNA特异性结合结构域的体外分子进化
13.The Influence of Extracorporeal Circulation on Th1/Th2, Tc1/Tc2 Differentiation and Transcription Regulatory Factors;体外循环对Th1、Tc1/Th2、Tc2细胞分化影响及其相关转录调节因子的研究
14.RNA Interferencing Krüppel Like Factor 8 Inhibits the Renal Carcinoma 786-0 Cells Growth in Vitro and in VivoRNA干扰Krüppel样转录因子8对肾癌786-0细胞体内外生长的影响
15.Experimental Study of the Effect of Reverse Transcriptase Inhibitor AZT in Rat C6 Glioma Cells in Vitro逆转录酶抑制剂AZT对大鼠C6胶质瘤细胞作用的体外研究
16.A telecast originating outside a studio.场外转播在录音棚之外录制的电视节目
17.Gene Transfer and Expression of Human Proinsulin Gene in HepG2 Cells in Vitro;含突变人胰岛素原基因逆转录病毒载体在体外培养的HepG2细胞中的基因转移和表达
18.Tissue Engineered Bone Formation Originating from Bone Marrow Stem Cell Transferred by Retroviral Vector Mediated hBMP-7 Gene in Vitro and Vivo;逆转录病毒介导hBMP-7基因转染兔骨髓基质干细胞体内外成骨实验研究
相关短句/例句

transcription in vitro体外转录
1.Digoxigenin(DIG) labeled RNA probes were prepared by transcription in vitro in order to establish ISH(in situ hybridization) method.根据hsp70和hsp90 mRNA基因序列,设计并合成引物,将PCR扩增的基因片段克隆到pGEM-T载体,重组质粒经筛选、鉴定,利用体外转录系统,制作地高辛标记的RNA探针,建立检测hsps mRNA原位杂交方法。
2.4 kb) which is complementary to both coding region and 5 terminus franking region (upstream the initiator codon AUG) have been obtained by transcription in vitro with SP6 RNA polymerase or T7 RNA polymerase from the plasmids, pSP72-C12 and pSP72-C1.体外转录载体pSP72—C12和pSP72—C1。
3.The Study of Amplifing HCV ssRNA by RT-PCR Combined With Transcription in vitro;本研究旨在以HCV为平台,在简化RT-PCR基础上,结合体外转录,建立一种特异、高效、简便的检测血清中HCVRNA的体外转录合成系统。
3)RNA transcripts体外转录体
1.The RNA transcripts were transfected into the virus-free G.本研究根据犬贾第虫病毒(长春株)基因序列设计一套含T7启动子的特异性重叠引物,构建犬贾第虫病毒基因组全长cDNA克隆,制备感染性犬贾第虫病毒体外转录体,采用体外转录体电穿孔转染和病毒粒子与无毒株滋养体共孵育两种感染方法进行感染试验,感染后每隔12h收取一次虫体,通过透射电镜观察病毒感染对无毒株滋养体细胞的影响。
4)In vitro transcription体外转录法
5)transcription of exosome核外体转录
6)RNA in vitro transcriptionRNA体外转录
延伸阅读

体外转录分子式:CAS号:性质:在试管中对一个结构基因5′一端加上启动子,3′端加终止子在四种核苷三磷酸存在下经RNA聚合酶作用即可以结构基因为样板进行转录,合成RNA。