1)Subtractive hybridization消减杂交
1.Cloning of Novel Genes Related Apoptosis by Improved, PCR-based Subtractive Hybridization;应用基于PCR的改良消减杂交技术克隆凋亡相关基因
2.BACKGROUND & OBJECTIVE: Subtractive hybridization technology is a common method to screen and clone differentially expressed genes.背景与目的:消减杂交技术是一种寻找和克隆差异基因的方法。
3.Methods In our study, SMART reverse transcription method, long-distance PCR, streptavidin magnetic bead-mediated subtraction and spin column chromatography were adopted on the basis of classical subtractive hybridization.目的 运用改良消减杂交技术构建大鼠脊髓损伤修复过程中出现的差异表达基因文库 ,为寻找在脊髓损伤后的修复过程中起关键作用的分子提供帮助。
英文短句/例句
1.Isolate the Genes Related to the Colorectal Carcinoma with Suppression Subtractive Hybridization;应用抑制消减杂交技术筛选大肠癌相关基因
2.Study on Screening of Differences between Indigenous and Foreign Pig Breeds Genomes by Suppressive Subtractive Hybridization;抑制消减杂交法筛选中外猪种基因组差异研究
3.Isolation of the Genes Related to Breast Cancer by Suppression Subtractive Hybridization;利用抑制消减杂交技术筛选乳腺癌相关基因
4.Application of Suppression Subtractive Hybridization in Cucurbits and Vegetables抑制消减杂交技术在瓜菜作物研究中的应用
5.An Experimental Study on the Screening of Lung Cancer Metastatic-related Genes by Suppression Subtractive Hybridization;抑制消减杂交技术筛选肺癌转移相关基因的实验研究
6.Cloning of Genes Related to Differentiation of Leukemia Cell Line K562 by cDNA Subtractive Hybridization;用cDNA消减杂交法克隆白血病细胞K562分化相关基因
7.Screening and Identification of Genes Transactived by p7TP3 Spliced Variant 1 by Suppression Subtractive Hybridization;应用抑制性消减杂交技术筛选p7TP3剪切体1反式激活基因
8.Study on Differential Expression Genes of Caski Cell Lines Induced by Exposing to the Space Environment by Suppression Subtractive Hybridization;利用抑制性消减杂交技术筛选太空诱变宫颈癌细胞差异表达基因
9.Cloning the Genes Differentially Expressed in Tremor Rat by Suppression Subtractive Hybridization;应用抑制性消减杂交克隆震颤大鼠差异表达基因
10.Study of Salt Tolerance Mechanism of Puccinellia Tenuiflora Using Suppression Subtractive Hybridzation and cDNA Microarray;抑制性消减杂交和cDNA微阵列技术研究星星草耐盐机理
11.Seperation of cDNA Fragments Related to Waterlogging Tolerance in Maize Roots Using Suppression Subtractive Hybridization and cDNA Microarray;利用抑制消减杂交和cDNA芯片技术筛选玉米耐渍相关的cDNA片段
12.Construction of Suppression Subtractive Hybridization Libraries of Tamarix.Spp. under Drought Stress and Analysis of Est;干旱胁迫下柽柳抑制性消减杂交文库的建立及EST分析
13.Screening and Cloning Tail-regeneration Related Genes of the Lizerd (Eremias Multiocellata) by Suppression Subtractive Hybridization;应用抑制消减杂交技术筛选与密点麻晰断尾再生相关的基因
14.Cloning of Metastasis-related Genes Expressed in Renal Cell Carcinoma by Suppression Subtractive Hybridization;抑制性消减杂交技术克隆肾细胞癌转移相关基因
15.Screening the Metastasis Functional Genes by Combining Suppression Subtractive Hybridization with cDNA Microarray;联合抑制消减杂交及基因芯片技术筛选肿瘤转移效应基因
16.Screening and cloning of the PS1TP5 transactivation related genes by suppression subtractive hybridization应用抑制性消减杂交技术克隆和筛选PS1TP5反式激活相关基因
17.Isolation of Fertility Restoration-related ESTs in Pepper Cytoplasmic Male Sterility Lines Using SSH利用抑制消减杂交技术分离辣椒细胞质雄性不育育性恢复相关EST
18.Construction and Analysis of Suppression Subtractive Hybridization Library of Dinoflagellate Alexandrium catenella链状亚历山大藻抑制消减杂交文库的构建及分析
相关短句/例句
cDNA Subtractive HybridizationcDNA消减杂交
1.Cloning of Genes Related to Differentiation of Leukemia Cell Line K562 by cDNA Subtractive Hybridization;用cDNA消减杂交法克隆白血病细胞K562分化相关基因
3)suppression subtractive hybridization(SSH)抑制消减杂交
1.Application of suppression subtractive hybridization(SSH) to study of cold tolerance in common carp(Cyprinus carpio);抑制消减杂交技术在鲤鱼抗寒研究中的应用
2.Differentially expressed cDNAs subtracted female from male gametophytes of Laminaria japonica were obtained by suppression subtractive hybridization(SSH).利用抑制消减杂交法(suppression subtractive hybridization,SSH)获得了海带雌、雄配子体差异表达的cDNA片段,将雄配子体差异表达的cDNA片段克隆于pGEM-T载体并转化大肠杆菌(Escherichia coli)JM109,构建雄配子体差异表达的cDNA消减文库。
3.The subtractive cDNA libraries for RH strain(typeⅠ) and QHO strain(typeⅡ) were constructed by the technique of suppression subtractive hybridization(SSH) using the PCR-Select cDNA Subtraction Kit.用抑制消减杂交技术(SSH)构建弓形虫强弱毒株正向消减(弱毒株消减强毒株)及反向消减(强毒株消减弱毒株)cDNA文库。
4)suppression subtractive hybridization抑制性消减杂交
1.Screening and cloning of target genes transactivated by human gene C1 encoding protein 1 interacting with HBV core antigen using suppression subtractive hybridization technique;抑制性消减杂交技术筛选乙型肝炎病毒核心蛋白相互作用蛋白编码基因C1的反式调节基因
2.Construction of cDNA library of hepatitis B virus with X protein C-terminally truncated 40 amino acids by suppression subtractive hybridization method;抑制性消减杂交构建乙型肝炎病毒X蛋白羧基端缺失40个氨基酸cDNA文库
3.Identification of genes associated with the metastasis of adenoid cystic carcinoma by suppression subtractive hybridization;应用抑制性消减杂交技术分析涎腺腺样囊性癌转移相关基因
5)Suppression subtractive hybridization抑制消减杂交法
1.Suppression subtractive hybridization (SSH) method was employed to analyze the differently expressed cDNA sequence between the strained VSMCs pretreated with and without wortmannin, a specific inhibitor of PI3K.方法应用FX-4000T细胞应变加载系统,对大鼠主动脉VSMCs施加1Hz、10%张应变,Western blotting检测细胞在不同加载时间下Akt磷酸化水平的变化;用抑制消减杂交法(SSH),筛选给予PI3K的抑制剂Wortmannin和给予DMSO两组细胞在加载张应变24h后的差异表达基因片段,得到的消减杂交产物连接到T载体上,经过蓝白斑筛选,对所有阳性克隆进行菌液PCR筛选及测序,应用BLASTN进行序列比对。
6)SSH抑制性消减杂交
1.Screening Correlative Genes from Citrinin Biosynthetic Pathway in Monascus Species of by SSH;应用抑制性消减杂交法筛选与红曲菌产桔霉素相关的基因
2.Methods:Suppression Subtractive Hybridization(SSH) was performed to profile differentially express genes between normal abortive fetus (as the driver) and Down s syndrome fetus(as the tester),then screening and analyzing the subtractive libraries.方法:利用抑制性消减杂交的方法对DS胎儿及正常胎儿脑组织样品进行正向消减杂交,利用斑点杂交对所获的基因片断进行进一步的验证。
3.coli132(UPEC132)by suppression subtractive hybridization(SSH).目的:应用抑制性消减杂交技术,构建致肾盂肾炎大肠埃希菌132株(UPEC132)与已完成基因组测序的非致病性大肠埃希菌标准株MG1655的基因组DNA消减文库。
延伸阅读
消减1.减少;降低标准。 2.谓消瘦。
