1)reverse transcription逆转录
1.Process of HIV-1 reverse transcription and its detection by using PCR;HIV-1逆转录过程及应用PCR检测
2.The germline mutations of MLH1 and MSH2 mRNA were detected by cDNA sequencing analysis following reverse transcription-PCR(RT-PCR) with special primers, heat-resistance reverse transcriptase, and expand long template PCR.方法收集符合Amsterdam标准Ⅱ的12个家系14名家庭成员外周血,用特异引物和耐热性逆转录酶特异地逆转录MLH1和MSH2的RNA;利用长模板PCR扩增酶扩增逆转录产物(cDNA);测序分析扩增产物。
3.The process of reverse transcription can be divided into several steps:formation of minus-strand strong-stop DNA;the first translocation; initiation of plus-strand DNA synthesis;the second translocation and the completion of both strands.人类免疫缺陷病毒(Human Immunodeficiency Virus,HIV)是逆转录病毒,进入宿主细胞胞浆即以其正链RNA基因组为模板,在逆转录酶的作用下开始逆转录合成双链的DNA。
英文短句/例句
1.reverse transcriptase【生化】逆转录酶, 反转录酶
2.highly active antiretroviral therapy (HAART)高效抗逆转录病毒疗法
3.cyclical transcription and reverse transcription循环(式)转录(和)逆转录(反应)
4.avian reverse transcriptase禽源逆转录酶[有时特指禽成髓细胞瘤病毒逆转录酶]
5.Detection of rubella virus gene sequences by RT-nested PCR用逆转录套式PCR检测风疹病毒RNA
6.avian myeloblastosis virus reverse transcriptase禽成髓细胞瘤病毒逆转录酶
7.Studies on the Screening Method of HIV-1 Reverse Transcriptase Inhibitors;HIV-1逆转录酶抑制剂筛选方法的研究
8.Design, Synthesis and Evaluation of Benzimidazole Derivatives as a Novel Class of HIV Non-nucleoside Reverse Transcriptase Inhibitors (NNRTIs);新型HIV非核苷类逆转录酶抑制剂(NNRTIs)
9.Studies on a Novel Non-Nucleoside Reverse Transcriptase Inhibitor--Nevirapine;新型逆转录酶抑制剂Nevirapine的研制
10.Expression of telomerase reverse transcriptase mRNA in oral lichen planus and oral squamous cell carcinoma端粒酶逆转录酶mRNA在OLP和OSCC中的表达
11.Construction and Identification of Retroviral Vector Expressing TH Gene重组逆转录病毒pLNCX2-TH的构建及鉴定
12.Cloning of the Human Telomerase Reverse Transcriptase Core Promoter人端粒酶逆转录酶核心启动子的克隆
13.Study on Gene Transfer Mediated by Recombinant Retrovirus-Spermatogonial Stem Cells重组逆转录病毒-精原干细胞介导的转基因研究
14.Studies on the Production of Transgenic Chicken via Retrovirus Vectors逆转录病毒为载体制备转基因鸡的初步研究
15.Transcription of antisense RNA of hepatitis B virus through retroviral mediated gene transfer逆转录病毒载体介导乙型肝炎病毒反义基因的转录表达
16.amphotropic retrovirus兼嗜性逆转录病毒,双嗜性逆转录病毒[既能在宿主细胞又能在异种细胞上引起产毒性感染]
17.Construction of Retroviral Vector Expressing Enhanced Green Fluorescent Protein Gene Driven by Human Telomerase Reserve Transcriptase Gene Promoter;人端粒酶逆转录酶基因启动子调控绿色荧光蛋白基因的逆转录病毒载体的构建
18.Detection of SARS-CoV from feces samples with RT-PCR逆转录套式PCR检测粪便样本中的SARS冠状病毒
相关短句/例句
Reverse transcript逆转录
1.Methods We used reverse transcript,nested PCR and southern hybridization with α fetoprotein mRNA which can be symbolized in hepatocellular carcinoma(HCC).方法 采用逆转录和巢式多聚酶链式反应及Southern杂交技术 ,将AFPmRNA作为肝癌细胞的特异标志 ,特异扩增检测肝癌患者外周血中微量转移的肝癌细胞。
3)RT-PCR逆转录PCR
1.Cloning and Sequencing of Tumor Suppressor Gene LKB1/STK11 Associated with Peutz-Jeghers Syndrome by RT-PCR;PJ综合症相关抑癌基因LKB1/STK11的逆转录PCR法克隆及序列分析
2.Methods Expression of PLC-epsilon in bladder tissues of 27 patients with TCCB and 12 normal bladder tissues was detected by RT-PCR,and its relationship with clinicopathological characteristics of tumor was analyzed.方法采用逆转录PCR(RT-PCR)方法检测27例膀胱移行细胞癌和12例正常膀胱对照组中PLCε mRNA表达,分析其与膀胱病理学特征的关系。
3.Me thods Total RNA was isolated from rat brain tissue, and the coding region of DNA polymerase β was amplified by reverse transcriptase - polymerase chain reaction (RT-PCR).方法提取大鼠脑组织总RNA,用逆转录PCR扩增DNA多聚酶β(POLB)的编码区,与T载体连接,作DNA测序后,酶切POLB基因并连入腺病毒穿梭质粒pAdTrack-CMV,电转至含腺病毒pAdeasy骨架质粒的大肠杆菌BJ5183。
4)reverse transcriptase逆转录酶
1.RT-PCR kit with different reverse transcriptase affected detecting result of COX-2 mRNA expressoin;不同逆转录酶的RT-PCR反应体系影响COX-2 mRNA表达的检测结果
2.Progress in HIV non-nucleoside reverse transcriptase inhibitors (NNRTIs);HIV非核苷类逆转录酶抑制剂研究进展
3.Expression of telomerase reverse transcriptase and its significance in human fetal lung development;人胎肺发育不同阶段端粒酶逆转录酶的表达及意义
5)reverse transcription polymerase chain reaction逆转录PCR
1.Methods The expression of SSTR subtype 1~5 mRNA was determined by reverse transcription polymerase chain reaction method in 30 cases of pancreatic cancer and in 12 cases of normal pancreas tissues.方法应用逆转录PCR(RT-PCR)检测30例胰腺癌组织和12例正常胰腺组织生长抑素受体亚型mRNA的表达。
6)RT-PCR逆转录-PCR
1.The expression of multidrug resistance(mdr 1) gene was tested by RT-PCR and Western blot.方法采用MTT法检测X射线照射后CNE-1细胞对顺铂(cD-DP)的耐药性;采用逆转录-PCR(RT-PCR)和蛋白免疫印迹(W estern b lot)方法,检测照射后CNE-1细胞mdrl基因的表达。
延伸阅读
逆转录分子式:CAS号:性质:又称逆转录。以RNA为模板产生DNA分子的过程。
