1)cloning vector克隆载体
1.Construction of cloning vector of c-myc-TFF2 fusion gene by PCR based gene assembly;基因拼装构建c-myc-hTFF2融合基因克隆载体pBS-TFF2
2.The required fragments were selected with correspording restriction endonuclease and inserted into suitable cloning vector by two steps.用碱裂解法抽提质粒后，利用相应的限制性内切酶切出目的基因片段，连接到合适的载体上，构建了完整的ＭＤＶＢ抗原基因克隆载体。
3.A protruding T on 3blunt end of the cloning vector was created by adding dTTP and Taq DNA polymerase to the reaction mixtures.将克隆载体平端切割后，向反应体系中加入ｄＴＴＰ，用ＴａｑＤＮＡ聚合酶处理载体，使其３－端带上一个凸出的单碱基Ｔ，修饰后的载体可直接与纯化的ＰＣＲ产物连接。
英文短句/例句
1.These are inserted into a cloning vector.它们被插入克隆载体。
2.Construction and identification of the cloning vectors for neutrophil gelatinase-associated lipocalin geneNGAL基因克隆载体的构建与鉴定
3.Gene Splicing and Construction of PRKAG2 Gene TA Cloning Vector人PRKAG2全长基因的体外拼接及TA克隆载体的构建
4.Cloning of Human Noggin cDNA and Construction of Mammalian Expression Vector PCS2+[Tα1]-Noggin;人noggin基因克隆及真核表达载体构建
5.Cloning of Expansin Gene in Chimonanthus Praecox and Construction of Expression Vector;蜡梅expansin基因的克隆及表达载体的构建
6.Cloning of Human CD200 Gene and Construction of Eukaryotic Expression Vector;人CD200基因克隆与真核表达载体的构建
7.Cloning and Construction of Recombinant Adenovirus Vector of nm23-H1;Nm23-H1cDNA片段克隆及腺病毒载体的构建
8.Clonging of Two Bt Genes and Construction of Plant Expression Vector in Which Fused the Bt Genes;双Bt基因克隆与植物表达载体的构建
9.Gene Construction of Adenoviral Vector-Mediated Vascular Endothelial Growth Factor 121;AD载体介导的VEGF121基因克隆的构建
10.Cloning of BBE gene of Papave somniferum and construction of its RNAi expression vector野生罂粟BBE基因克隆及RNAi载体构建
11.Cloning of Pollen Fertility Gene MS2 and Construction of Its RNAi Expression Vector花粉育性基因MS2的克隆与RNAi载体构建
12.Clonging of PRO Gene from Aromatic Rice and Constructing of Its Expression Vector香稻PRO基因的克隆及表达载体的构建
13.Cloning of Hal1 gene and construction of its expression plasmid耐盐基因Hal1的克隆及表达载体的构建
14.Cloning, Expression, Purification and Polyclonal Antibody Preparation of Human Apolipoprotein A5人载脂蛋白A5基因的克隆、表达、纯化及多克隆抗体制备
15.pICln Gene Cloning, the Eukaryotic Expression Vector Construction and Polyclonal Antibody Preparation;pICln基因克隆、真核表达载体构建及抗体制备
16.Cloning and constructing of Os-miR390 precursor gene and transforming it into rice水稻Os-miR390前体基因的克隆、载体构建及转化
17.ARDRA Analysis, Antagonistic Activity of Siderophore Analysis and fur Gene Cloning of Siderophore-Producing Bacteria of Tobacco Rhizosphere;烟草根际铁载体产生菌ARDRA分析、铁载体拮抗活性研究及fur基因克隆
18.Cloning and Eukaryotic Expression Vector Constructing of Rat s Fas Gene;大鼠Fas基因克隆及重组真核表达载体的构建
相关短句/例句
TA cloning vectorTA克隆载体
1.Construction of the recombinant pET15b-SOD1 plasmid with TA cloning vector;利用TA克隆载体构建pET15b-SOD1重组质粒
2.Construction of rat antisense TIMP-1 gene TA cloning vector;大鼠金属蛋白酶组织抑制物-1反义基因片段TA克隆载体的构建
3.The PCR product was cloned into TA cloning vector.结论 成功地构建了 HLA-DRα基因片段TA克隆载体。
3)PUC18 cloning vectorPUC18克隆载体
4)T A cloning vectorT-A克隆载体
5)vector T cloningT载体克隆
6)E. coli cloning vectorE.coli克隆载体
延伸阅读
植物克隆载体分子式：CAS号：性质：植物基因操作中之携带外来DNA的载体，并以可遗传的方式将它嵌入植物细胞之内。天然的载体有根癌根杆菌(Agrobacterium tumefaciens)的Ti质粒或发根农杆菌(A. rhizogenes)的Ri质粒以及DNA植物病毒。现在因Ti或Ri质粒太大，不易操作，常取其DNA复制及引发癌肿的基因与普通细菌质粒组成穿梭质粒，便于先在细菌(尤其是大肠杆菌)中操作、扩增，然后再引入植物。
