反转录,reverse transcription英语短句,例句大全

反转录,reverse transcription
1)reverse transcription反转录
1.cDNA was synthesized by reverse transcription, and cDNA fragments larger than 400 bp were ligated with dephosphorylated arms of Agt11.方法:从9901细胞中提取总RNA,分离mRNA,反转录合成双链cDNA,末端削平,和EcoR I适配子连接。
2.In this study reverse transcription coupled with the polymerase chain reaction(RT PCR) has been established to detect hog cholera virus (HCV).本研究建立了反转录聚合酶链反应 ( RT PCR)技术检测猪瘟病毒 ( Hog Cholera Virus,HCV)方法。
3.A new strain of EHDV2 was studied by a series of experiments including extraction of RNA,reverse transcription, clone, PCR,purifiction of plasmid.用美国新分离到的一株鹿流行性出血热血清Ⅱ型毒株，通过繁毒，提核酸，反转录，ＰＣＲ扩增，克隆，质粒增殖提纯，最后应用７脱氮ｄＧＴＰ试剂盒测定其ＶＰ７核酸基因序列。

英文短句/例句

1.reverse transcriptase【生化】逆转录酶, 反转录酶
2.Cloning of Reverse Transcriptase of Retrotransposons and Stress-Tolerant Related Gene in Wheat;小麦反转录转座子反转录酶和抗逆相关基因的克隆
3.That is especially true for antiretroviral therapy.这些对于反转录酶疗法的确相当正确。
4.Characterization of Porcine Endogenous Retrovirus in BaMa Miniature Pig;巴马小型猪内源性反转录病毒的特性
5.Isolation and Characterization of LTR Retrotransposon Sequences from Strawberry Genome;草莓基因组中LTR反转录转座子的分离和鉴定
6.Study on PCR Reaction System of Inter-retrotransposon Amplified Polymorphism(IRAP) in Lentinula edodes香菇反转录转座子间扩增多态性(IRAP)PCR反应体系的研究
7.HIV (in full human immunodeficiency virus):Retrovirus associated with AIDS.人类免疫不全病毒:与爱滋病相关的反转录病毒。
8.Cloning of bovine sox2 gene and construction of its retrovirus vector牛sox2基因的克隆及重组反转录病毒载体的构建
9.Detection potato virus by microarray and RT labeling method用反转录标记法制备马铃薯病毒的检测芯片
10.An analysis of the relationship between the drug resistance and A181 mutation of the hepatitis B virus reverse transcriptaseHBV反转录酶A181位点变异与临床耐药的关系
11.Construction and identification of recombinant retroviral vector expressing shRNA targeting human MDC1人MDC1基因shRNA反转录病毒载体的构建及鉴定
12.Many of the plant retrotransposons are transcriptionally activated by various biotic and abiotic stress factors.在一些生物的和非生物的逆境条件下 ,反转录转座子的转录可以被激活。
13.Studies on the Detection of Bovine Viral Diarrhea-mucosal Disease Virus Using Reverse Transcription-nested Polymerase Chain Reaction;反转录—套式聚合酶链式反应检测牛病毒性腹泻/粘膜病病毒的研究
14.Studies on the Detection and Differentiation of Virulent and Avirulent Strains of Newcastle Disease Virus in Chickens Using Reverse Transcription-Nested Polymerase Chain Reaction;反转录—套式聚合酶链反应检测、鉴别鸡新城疫病毒强、弱毒株的研究
15.Rapid Detection of Tobacco Mosaic Virus from Crude Samples on Oscillatory-flow Reverse Transcription Polymerase Chain Reaction Microfluidics微流控振荡流反转录聚合酶链式反应快速检测烟草花叶病毒
16.Study on Multiplex Reverse Transcription Polymerase Chain Reaction for Detection of H5,H7 and H9 Subtypes Avian Influenza Virus多重反转录聚合酶链式反应检测H5、H7和H9亚型禽流感病毒的研究
17.Study on Short-Term Adverse Effects of Highly Activity Antiretroviral Therapy in Children with Acquired Immunodeficiency Syndrome高效抗反转录病毒治疗艾滋病患儿近期不良反应71例
18.The Roles of the Copia-retrotransposons in Wheat on the Biological Effect of the Low-energy N～+ Beam Implantation;小麦反转录转座子在低能N～+离子束注入生物效应机制中的作用

相关短句/例句

RT-PCR反转录PCR
1.Methods: The expression of 5-HT receptor subtype mRNAs was detected in the lumbar dorsal root ganglion (DRG) by reverse transcription-polymerase chain reaction(RT-PCR) following unilateral injection of formalin into the plantar surface of rat hind paw.方法:用反转录PCR技术观察大鼠单侧足底皮下注射福尔马林致痛后背根节内5-HT1~7受体亚型mRNAs的表达变化。
2.?Methods Using the total RNA extracted from fresh rat hippocampus as template,the cDNA of PDZ1 was amplified by RT-PCR;the cDNA obtained was subsequently cloned into the shuttle vector pAdTrack-CMV(CLONTECH),and then,the sequence of the product was determined.方法以异硫酸氢胍-酚-氯仿一步法提取的总RNA为模板,采用反转录PCR(RT-PCR)法获得PDZ1的cDNA;与腺病毒穿梭载体用T4DNA连接酶连接;连接产物转化大肠杆菌JM109进行筛选、序列测定。
3.Methods:BMP7 gene was cloned from the HEK293 cells with RT-PCR and nested-PCR skills,and after being repaired,the whole cDNA of BMP7 was obtained.方法 :通过反转录PCR技术和巢式PCR技术从人类胚胎肾 2 93细胞中克隆BMP7基因 ,应用互补拼接的方法对其进行修复获得全长cDNA ,将其与T载体进行连接得到BMP7克隆质粒。
3)Reverse transcriptase反转录酶
1.OBJECTIVE:To investigate the diagnostic meaning of the human tolomerase reverse transcriptase (hTERT) gene expression level in peripheral blood mononuclear cells in non-Hodgkin’s lymphoma(NHL) patients.目的 :探讨人外周血单个核细胞端粒酶反转录酶 (hTERT)基因的表达水平对于非霍奇金淋巴瘤 (NHL)诊断的意义。
2.This study analyzed the characteristics of reverse transcriptase sequences of copia like retrotransposons in Amaranthus via PCR amplification and sequence approaches.利用籽粒苋为材料 ,通过PCR扩增、克隆研究了copia类型反转录转座子的反转录酶序列在籽粒苋中的表现 ,结果表明 :(1)反转录转座子在苋属的 30个品系中同时检测到 ,说明反转录转座子在籽粒苋的不同种中普遍存在 ;(2 )对野生苋 (A 。
3.In order to study the structural elements within the ε that is essential for initiating priming of HBV reverse transcriptase(P protein),all selected aptamers were subjected to in vitro priming assay and RNA secondary structure probing.通过构建鸭乙肝病毒ε(Dε)的RNA文库并利用指数级富集的配体系统进化技术(SELEX)筛选的策略,获得与反转录酶(P蛋白)高度亲和的适配子(Aptamer),再通过体外引发实验和核酸酶切割方法测定全部适配子的RNA二级结构,以研究鸭乙型肝炎病毒(Duck Hepatitis B Virus,DHBV)中对启动P蛋白引发步骤至关重要的ε结构信息。
4)RT-PCR反转录-PCR
1.0kb DNA fragment was obtained by RT-PCR fr.本研究以黑曲霉M-1为出发菌株，设计特异的引物，采用反转录-PCR技术直接从黑曲霉总RNA中反转录并扩增出约3。
5)RT PCR反转录PCR
1.cDNA fragment encoding human P34H was amplified by RT PCR using specific primers, and then was cloned into pGEM T vector.　方法 :提取人附睾体部总RNA ,并以此为模板 ,进行反转录PCR获得编码P34H蛋白的基因片段。
2.Methods:Tumor tissues and adjacent normal tissues in 41 cases of esophageal carcinoma were studied by using the reverse transcriptase polymerase chain reaction(RT PCR).方法 :用反转录PCR(RT PCR)法检测 41例食管癌患者的癌组织、癌旁组织MMP 2mRNA的表达。
6)Reverse transcription PCR反转录PCR