1)colony PCR菌落PCR
1.False positives resulted from colony PCR in rapid identification of recombinant clones from phage antibody library;菌落PCR快速分析抗体库重组率时的假阳性现象
2.Selection and identification of recombinant plasm ids using colony PCR method;菌落PCR技术在重组质粒筛选与鉴定中的应用
3.To establish a quick, sensitive and economical PCR method for directly detecting tlh and adh genes-carrying Vibrio parahaemolyticus in environment and seafood, a colony PCR method was developed and its results were compared with those of the conventional PCR method, thus establishing a colony PCR method.为建立一种快速、经济、敏感的PCR检测方法 ,以直接检测出环境、海产品等中的靶基因 ,通过PCR扩增已知携带tlh和tdh基因的副溶血弧菌标准菌株 ,并和常规PCR方法比较 ,初步建立了菌落PCR技术 ;再以副溶血弧菌标准菌株为阳性对照 ,以本实验室自海洋环境中分离得到的数株野生菌株为检测对象 ,采用新建立的菌落PCR以及常规PCR方法对它们进行扩增比较 ,进一步证明了菌落PCR技术检测携带相关基因菌株的可靠性 。
英文短句/例句
1.Study on Improvement Effect of Triton X-100 for Individual Bacterial Colony PCR MethodTriton X-100在单菌落PCR技术中的优化作用
2.Application of Fluorescent Quantitative PCR in Research of Mycobacterium Tuberculosis Microcolony Culture荧光定量PCR在结核杆菌微菌落培养研究中的应用
3.Bacterial Community Composition in the Sea Studied by PCR-Denaturing Gradient Gel Electrophoresis;应用PCR-DGGE技术分析长江口低氧区和黄海冷水团的细菌群落组成
4.PCR-DGGE Analysis of the Bacterial Communities in Paddy Fields in Fujian Province and Cloning of Oil-degerading Genes;福建稻田细菌群落PCR-DGGE分析和石油烃降解基因克隆
5.Study on Community and Genetic Diversity of Soil Bacteria under Phyllostachy Pubescens Stands by PCR-DGGE应用PCR-DGGE方法研究毛竹土壤细菌群落结构及其遗传多样性
6.Diversity of Bacterial Community Structure in Soils of Loess Plateau黄土高原土壤中细菌群落结构多样性的PCR-DGGE分析
7.PCR Ampification and Sequence Analysis of rDNA-ITS of Corynespora cassiicola from Rubber橡胶棒孢霉落叶病菌rDNA-ITS区的PCR扩增与序列分析
8.PCR-DGGE analysis of bacterial communities in marine Litopenaeus vannamei culture system凡纳滨对虾海水养殖系统内细菌群落的PCR-DGGE分析
9.Effects of Swine Manure Compost with Inoculation of Different Microorganism Agents and Research on Microbial Community Changes by PCR-DGGE;不同菌剂处理对猪粪堆肥效果的影响及PCR-DGGE研究堆肥微生物群落变化
10.daughter colony子菌落[由原生菌落续发生长的小菌落]
11.Rapid Immuno-PCR Detection of Acidovorax avenae subsp.citrulli.西瓜细菌性果斑病菌快速免疫PCR检测
12.A combination of different PCR assays for the detection of Yersinia pestis不同PCR联合用于鼠疫耶尔森菌的检测
13.Rapid identification of Yersinia pestis by multiplex-PCR多重PCR快速鉴定鼠疫耶尔森氏菌
14.Detection of the RTX toxin gene in Vibrio cholerae by multiplex PCR多重PCR检测霍乱弧菌RTX毒力基因
15.Rapid Detection of Enteropathogen with PCR Assay;快速检测肠道致病菌的PCR技术的研究
16.The Study of the Flourecent Quantitative PCR Method in Detection of Bacteria Fragilis;荧光定量PCR检测脆弱类杆菌的研究
17.Morphology of the Pathogen of Citrus Huanglongbing in Japan and It s Detection by PCR;日本柑桔黄龙病菌电镜观察及PCR检测
18.Rapid Detection of Xanthomonas Axonopodis Pv. Citri Vauterin Et Al. by Polymerase Chain Reaction Assay;柑桔溃疡病菌的PCR快速检测技术研究
相关短句/例句
bacterial colony PCR菌落PCR
1.In this paper,the combination of bacterial colony PCR and denaturing gradient electrophoresis were used in the microbial diversity analysis of 87 halo inhabited bacterial culture collections.利用菌落PCR方法,结合DGGE的检测方法,对所保存的87份盐生菌的培养物进行了多样性检测,初步研究显示至少有31株不同的菌种,表明新疆盐生环境存在较为丰富的菌种资源,同时表明菌落PCR结合DGGE技术为细菌鉴定和菌种保藏过程中重复菌株的去除提供了一种快速可靠的手段。
2.coli rapidly and reliably on a large scale,which combined the bacterial colony PCR method and the plasmid rapid identification method validly.针对如何大规模地筛选大肠杆菌重组子,将质粒快检和菌落PCR两种方法巧妙地融合,建立一种新的高效快捷的重组质粒筛选方法。
3)individual bacterial colony PCR单菌落PCR
1.The individual bacterial colony PCR method was used to screen the recombinant plasmids containing foreign genes and homologous recombinants directly.在利用单菌落PCR法直接筛选含有外源基因的重组质粒以及发生同源重组的重组子时,通过0。
4)yeast colony PCR酵母菌落PCR
1.Recombinants were identified by yeast colony PCR, then were induced by methanol and detected by SDS-PAGE analysis.berezovskii)白介素-2(interleukin-2,IL-2)到酵母表达载体pPIC9K,SalⅠ酶切后转化毕赤酵母GS115,G418抗性筛选高拷贝克隆,再由酵母菌落PCR鉴定;阳性克隆用甲醇诱导表达,SDS-PAGE分析,结果在诱导至第6d的酵母上清浓缩液中,检测到与预测林麝IL-2分子量相接近的,分子量约为18Ku的诱导表达带;TRIzol提取表达期间的酵母总RNA,并通过RT-PCR扩增IL-2mRNA,发现表达期的重组酵母细胞中存在IL-2mRNA,而对照没有检出,表明林麝IL-2存在低水平的表达。
2.Recombinants were identified by yeast colony PCR.5K上,SalI线性化后转化毕赤酵母GS115,418抗性筛选高拷贝克隆,再由酵母菌落PCR鉴定;阳性克隆用甲醇诱导表达,Tricine-SDS-PAGE分析,结果在诱导第2d的酵母裂解液中检测到与预期的SMAP-29分子量接近,约3。
5)Individual bacterial colonies PCR单菌落PCR法
6)Colony multiplex PCR菌落多重PCR
延伸阅读
大肠杆菌不同生化突变型菌落(显示不同颜色)
