1)cloning and sequencing克隆测序
1.Point mutations of p53 gene exon 7 were detected by cloning and sequencing assay.通过克隆测序的方法检测p53基因外显子7上的点突变。
2.Direct cloning and sequencing of restricted fragments with cohesive-ends by means of TA cloning;对限制性酶切片段的粘性末端先用T4DNA聚合酶处理,变为平末端,然后用TaqTMDNA聚合酶在其3′末端加上A腺苷,即可利用T/A克隆载体进行直接克隆测序。
英文短句/例句
1.The Cloning and Sequence Analysis of Interferon Induced Transmembrane Protein 1 GeneIFITM1克隆测序及生物信息学分析
2.Cloning and Sequencing of Partial Fragment of TNF-α Gene of Bos grunniens牦牛肿瘤坏死因子-α基因部分片段的克隆测序
3.Studies on FSHR Gene Polymorphism and ADD1 Gene Cloning and Sequencing of Goat;山羊FSHR基因多态性及ADD1基因的克隆测序研究
4.Cloning Sequence of MC1R Gene in Duck and AFLP Markers of Plumage Color in Mule Duck;鸭MC1R基因的克隆测序与半番鸭羽色AFLP分子标记
5.Total DNA Isolation of Termitomyces robustus and Cloning of its ITS Region粗柄鸡枞菌总DNA提取及ITS区克隆测序研究
6.Cloning and Prokaryocyte Expression of Hemagglutinin Protein Gene of Canine Distemper Virus犬瘟热病毒h基因克隆测序与原核表达
7.Isolation and Identification the Infectious Laryngotra-cheitis Virus HNchangge Strain of gC Gene鸡传染性喉气管炎病毒gC基因克隆测序
8.Cloning and Sequence Analysis of NS Gene and M Gene of Rabies Virus Isolates from Guangxi;广西狂犬病毒NS基因和M基因的克隆测序与序列分析
9.Cloning and sequencing of mic2/CD_(99) Genemic2/CD_(99)基因克隆和测序
10.Cloning and Sequencing of MT cDNA Gene in Housefly Larvae;家蝇金属硫蛋白的cDNA克隆与序列测定
11.Cloning, Sequence Analysis and Detection by in Situ Hybridization of Chicken Interleukin-1 β cDNA;鸡IL-1βcDNA克隆、序列分析及原位杂交检测
12.Cloning, Sequencing and Expression of Goat Poxvirus P32 Gene山羊痘病毒P32基因克隆、测序及表达
13.Research on the Cloning and Sequencing of Cytb Gene in Liaoning Cashmere Goat辽宁绒山羊Cytb基因的克隆和序列测定
14.Cloning and sequence analysis of par-4 SAC genepar-4 SAC基因的克隆及序列测定
15.Cloning and Sequence Analysis of GDF9B Gene in Xiangdong Black Goats湘东黑山羊GDF9B基因的克隆与测序分析
16.Cloning and sequencing the 3′terminal of the actin genes in the brown planthopper,Nilaparvata lugens (St?l)褐飞虱肌动蛋白基因3′末端克隆与测序
17.Cloning, Sequencing and Expressing of Protective Antigen Gene of the Spike Glycoprotein of Transmissible Gastroenteritis Virus;TGEV纤突糖蛋白保护性抗原基因克隆、测序及表达
18.Cloning and Sequence Measuring of the Marker Linked to Seed Trait in Soft-seeded Pomegranate;石榴软籽性状基因连锁标记的克隆与测序
相关短句/例句
clone sequencing克隆测序法
1.Samples were detected by direct sequencing and clone sequencing,and the results were compared.目的:了解PCR产物直接测序法与克隆测序法在单核苷酸多态性(SNP)检测上的差别。
3)BAC clone sequencingBAC克隆测序
4)cloning and sequencing克隆与测序
5)ITS clone sequencingITS克隆测序
6)T-A cloning and sequencingT-A克隆测序
延伸阅读
DNA测序分子式：CAS号：性质：确定一段DNA中碱基排列的次序。一般用双脱氧测序法，另外还有一种马克西姆-吉尔伯特法，是依据四个碱基对不同试剂，如哌啶及肼的不同的反应能力实现的。可利用克隆载体上接头的两侧引入已知序列(例如Sp6，T7，T3的启动子序列)，当插入目的片段后，即可利用此已知序列的寡核苷酸作引物。常用于未知序列DNA片段的测序。
