1)U6 promoterU6启动子
1.METHODS: The vector expressing shRNA designated pU6 was constructed by U6 promoter.方法:扩增U6启动子构建shRNA表达载体pU6。
2.After randomly connecting an oligonucleotide and another one with 4-8 bases pairs interval, they were directionally inserted into plasmid pGenesil-1 with respectively U6 promoter and termination code, the common green fluorescence protein (EGFP) gene and Neo gene.方法:分别设计、化学合成6对寡核苷酸单链,经退火、连接和磷酸化后得到Hpa-shRNA双链,将两两随机间隔4~8个碱基后定向克隆入带有各自的U6启动子和终止码、共同的绿色荧光蛋白(green fluo-rescent protein,EGFP)基因和Neo基因的pGenesil-1中,构建3条含两段Hpa基因shRNA的pGenesil-1-Hpa-shRNA真核表达载体,转染卵巢癌细胞株SKOV3,流式细胞仪和荧光显微镜下观察转染效率,免疫组化分析Hpa蛋白表达。
3.U6 promoter was linked with the sequence coding NS-siRNA and the termination signal which RNA polymerase Ⅲ recognized.方法 :以小鼠组织基因组DNA为模板 ,PCR方法克隆小鼠编码U6snRNA基因的启动子 ,将NS -siRNA表达序列、PolⅢ识别的终止信号与U6启动子连接起来 ,并将其插入 pcDNA4 /C载体中。
英文短句/例句
1.Cloning,Sequence Analysis and Transcription Activity of a Chicken U6 Promoter禽U6启动子的克隆、序列分析及其驱动的siRNA表达
2.Construction of Double-effect Vector and Function Identification of U6 Promoter双效表达载体的构建及其U6启动子的功能效率鉴定
3.Inhibition hTERT by siRNA Expression Cassettes with U6/H1 Promoters in HepG2 in Vitro;体外构建U6/H1双启动子SECs及其对HepG2细胞端粒酶活性抑制的研究
4.promoter suppression启动子抑制，启动子阻抑[一个启动子抑制另一个启动子的转录]
5.core promoter element核心启动子元件，启动子核心元件
6.promoter occulsion启动子封阻[上游启动子对下游启动子的阻碍作用]
7.chicken ovalbumin upstream promoter鸡卵白蛋白上游启动子
8.Study on the Promoter Activity in the HCV 5 UTR Sequence;HCV 5 UTR启动子活性研究
9.Screening and Characterization of Promoters in Rice Promoter Trap Lines;利用水稻启动子捕获系筛选启动子的研究
10.Natural Compounds Induce Klotho Promoter Activity and Klotho Promoter Analysis天然化合物诱导Klotho启动子活性及Klotho启动子分析
11.The structure of the seed-specific promoter matches the characteristics of the promoter Ⅱ, having a basal promoter, initiator and upstream element.种子特异性启动子的结构符合Ⅱ类启动子的特点，具有基本启动子、起始子和上游元件。
12.Cloning and Functional Analysis of OsBTF3p, a Rice Gene Promoter Directing GUS Expression;水稻基因启动子OsBTF3p的克隆及其启动活性分析
13.Study on Promoter Sequence with Function for Transcription Initiation of BMPR-2 Gene;BMPR2基因启动子具有启动功能片段的研究
14.NEGATIVE PRIMING WITH THE PRIME CONTAINING MORE THAN ONE TARGET;启动刺激含有多个靶子条件下的负启动效应
15.Molecular Cloning and Activity Analysis of a Rice Gene Promoter OsBTF3p水稻基因启动子OsBTF3p的克隆和启动活性分析
16.The Preliminary Analysis of Core Sequences at Human Integrin β1 Promoter人β1整合素启动子核心启动序列初步分析
17.promoter damping启动子减弱（作用）[邻近的或插入的启动子对增强子活性的减弱作用]
18.The Molecular Mechanism Study of LPS Induced the uPA Promoter ElementsLPS诱导uPA启动子的分子机制的研究
相关短句/例句
human U_6 promoter人U6启动子
3)avian U6 promoter禽U6启动子
1.【Objective】 To cloning avian U6 promoter for construction of short interference RNA(siRNA) expression vectors in avian cells.【目的】分离用于禽源细胞中表达短干扰RNA(short interference RNA,siRNA)的禽U6启动子。
4)U6 Pol III promotorU6聚合酶III启动子
5)promoter/non-promoter启动子/非启动子
6)promoter[英][pr?'m??t?(r)][美][pr?'mot?]启动子
1.A DNA Autonomous Replication Origin with Promoter Function in Yeast;一个具有启动子功能的酵母DNA自主复制起始区
2.Construction of recombinant of luciferase promoter reporter gene of endothelial-overexpressed lipopolysaccharide-associated factor 1;人内皮细胞高表达脂多糖相关因子1启动子荧光素酶报告基因重组体的构建
3.Effect of Aspirin on humanα_2(Ⅰ) collagen promoter induced by rhCD40L;rhCD40L及阿司匹林对人I型胶原α_2(I)链启动子活性表达的影响
延伸阅读
启动子分子式：CAS号：性质：(一)在DNA分子上，可被RNA聚合酶识别并与之结合的一段序列，位于结构基因的上游，在那里使转录起动。强的启动子可转录出更多的nRNA。因而在重组工作菌中常使用强制的启动子，以期取得更多的表达量。（二）一种致癌因素，在癌变过程的第二阶段或多阶段机制中造成第二阶段（促进阶段）；一种辅助致癌物。
