1)Bait protein诱饵蛋白
1.Then the bait vector pGBKT7-GR was transformed into AH109 yeast cells and the expression of the bait protein was analyzed by Western blot.方法RT-PCR扩增GR结合域,克隆入pMD18-T,测序正确后,再亚克隆入诱饵载体pGBKT7中,再把构建好的诱饵载体pGBKT7-GR转化到酵母AH109细胞中,并用Western blot分析诱饵蛋白的表达情况,同时检测诱饵蛋白的毒性和自激活作用。
2.Objective To construct the bait expression vector pGBKT7RARα-V of retinoic acid receptor variant protein for screening the target proteins interacting with the bait protein through the yeast two-hybrid technique.方法PCR扩增RARα-V,克隆入诱饵载体pGBKT7中,将构建好的诱饵载体pG-BKT7-RARα-V转化到酵母细胞AH109中,并利用蛋白印迹法(Western Blotting)分析诱饵蛋白的表达情况。
3.PART 1 CONSTRUCTION AND IDENTIFICATION OF THE BAIT EXPRESSION VECTOR OF NLS-RARαAbstract Objective: To construct the bait expression vector pGBKT7-NLS-RARαof retinoic acid receptor variant protein, for screening the target proteins interacting with the bait protein through the yeast two-hybrid technique.方法PCR扩增NLS-RARα,克隆入诱饵载体pGBKT7中,将构建好的诱饵载体pGBKT7-NLS-RARα转化到酵母细胞AH109中,并利用蛋白印迹法(Western Blotting)分析诱饵蛋白的表达情况。
英文短句/例句
1.A Novel Auto-catalysis/Two-Hybrid System to Identify Protein-Protein Interactions Involving Post-Translational Modifications;自体乙酰化诱饵蛋白酵母双杂合筛选体系的建立
2.Construction of Rotavirus VP4 Protein to the RRS Screening System Vector;酵母双杂交RRS筛选系统中轮状病毒外壳蛋白VP4诱饵载体的构建
3.Construction and Preliminary Identification of Yeast Two-hybrid Bait Plasmids Carrying Vesicular Stomatitis Virus Glycoprotein cDNA FragmentVSV糖蛋白酵母双杂交诱饵载体的构建和初步鉴定
4.Construction and Identification on Bait Vector of Prion(prp105-125 flaw) in Two-hybrid System of Yeast朊蛋白(prp105-125缺失)酵母双杂交诱饵载体的构建和鉴定
5.Construction of a bait plasmid containing HBV PreS1 gene in a yeast two-hybrid system and evaluation of its toxicity and self-activationHBV PreS1蛋白酵母双杂交诱饵表达载体的构建及其毒性和自激活效应检测
6.CONSTRUCTION OF vp37 AND vp39 BAIT VECTOR AND DETECTION OF ITS SELF-ACTIVATING EFFECT IN YEAST TWO HYBRID SYSTEMWSSV囊膜蛋白vp37和vp39基因酵母双杂交诱饵载体的构建及其激活作用检测
7.lure, entice, or entrap with bait.用饵引诱，诱惑或者诱陷。
8.Evaluation of Soy Protein Concentrate (SPC) as a Dietary Protein Source of Chinese Mitten-handed Crab, Eriocheir Sinensis;大豆浓缩蛋白作为中华绒螯蟹饵料蛋白源的综合评价
9.A lure, such as a spoon or spinner, that is used for trolling.钓绳、诱饵曳钓法中用的诱饵，如匙状假饵或旋转匙状诱饵
10.To place a lure in(a trap)or on(a fishing hook.置诱饵于…在（网子里）或在（鱼钩上）放诱饵
11.A fishing lure that rotates rapidly.旋式诱饵快速旋转的钓鱼用的诱饵
12.A decoy used in catching animals, especially an artificial bait used in catching fish.诱饵，囵子用来捕捉动物的诱饵，尤指用来捕捉鱼的人造诱饵
13.live bait,ie small fish used to catch larger fish活饵（用作鱼饵引诱大鱼上钩的小鱼）.
14.A lure to which hooks are attached, used especially in angling.鱼饵系于鱼钩的诱饵，尤用于垂钓
15.EFFECTS OF DIFFERENT PROTEIN, FIBRE AND FAT LEVELS IN DIETS ON THE GROWTH OF LARGE-MOUTH BASS,MICROPTERUS SALMOIDES饵料中不同蛋白质、纤维素、脂肪水平对大口黑鲈生长的影响
16.EFFECTS OF DIETARY PROTEIN,FIBRE AND FAT ON THE GROWTH OF LARGEMOUTH BASS(MICROPTERUS SALMOIDES)饵料中不同蛋白质、纤维素、脂肪水平对加州鲈鱼生长的影响
17.A Preliminary Study on the Saving Effect of Protein by Fat in the Artificial Diets to Culture Epinephelus awoara青石斑鱼配合饵料中脂肪节约蛋白质效应的初步研究
18.Evaluation of protein and fatty acid nutritive value in 3 preys and artificial feed of juvenile Acipenser sinensis中华鲟幼鱼饵料生物及人工饲料的蛋白质和脂肪酸营养价值评价
相关短句/例句
decoy[英]['di:k??][美]['dik??]蛋白质诱饵
3)termite baits白蚁诱饵
1.Comparison of antiseptic effects of mould inhibitors of termite baits.;几种白蚁诱饵防霉剂的防霉效果比较
4)termite bait白蚁诱饵剂
1.The termite baits were maded by the handmade molding instrument,and the crackle degree of termite baits was regarded as the evaluation index.笔者在多次试生产白蚁诱饵剂的经验基础上 ,通过模拟电动成型机制作出手工成型器 ,并用它来制作白蚁诱饵剂 ,分别对甘蔗粉诱饵剂和松木屑诱饵剂的成型工艺进行了研究 ,研究结果为土栖堤坝白蚁诱饵剂在电动成型机中大批量生产提供成型工艺方面的参考。
5)protein[英]['pr??ti:n][美]['protin]饵料蛋白质
1.Effects of dietary protein levels on growth,survival,body composition and hepatopancreas histological structure of the white shrimp,Litopenaeus vannamei,at different ambient salinities;不同盐度下饵料蛋白质含量对凡纳滨对虾生长、体成份和肝胰腺组织结构的影响
6)protein-rich diet高蛋白饵料
延伸阅读
普通免疫球蛋白 ,人血丙种球蛋白,丙种球蛋白药物名称：丙种球蛋白英文名：γ-Globulin别名： 免疫血清球蛋白;普通免疫球蛋白 ,人血丙种球蛋白,丙种球蛋白外文名：γ－Globulin 适应症： 含有健康人群血清所具有的各种抗体，因而有增强机体抵抗力以预防感染的作用。主要用于免疫缺陷病以及传染性肝炎、麻疹、水痘、腮腺炎、带状皰疹等病毒感染和细菌感染的防治，也可用于哮喘、过敏性鼻炎、湿疹等内源性过敏性疾病。 用量用法： 肌注：每次２～５ｍｌ，３周１次，用于内源性过敏性疾病，每次１０ｍｌ（含量１０％者），３周内注射２次。胎盘球蛋白每次６～９ｍｌ。 注意事项： 1.按球蛋白来源可分为两种，一为健康人静脉血来源的丙种球蛋白制剂，按蛋白质含量有１０％、１６％、１６．５％等数种（国内制品浓度在１０％以上），其中丙种球蛋白占９５％以上。另一种为胎盘血来源的丙种球蛋白（人胎盘血丙种球蛋白），即胎盘球蛋白，含蛋白质５％，其中丙种球蛋白占９０％以上。胎盘球蛋白因丙种球蛋白含量以及纯度均较低，其用量应相应增大。 2.除专供静注用的制剂外，一般制剂不可静注。 3.注射大量时，可见局部疼痛和暂时性体温升高。 注：参见：“丙种球蛋白注射液”. 类别：免疫增强剂（免疫反应调节剂）
