1)Taq DNA polymeraseTaqDNA聚合酶
1.coli DH5α was transformed with p Taq expressing plasmid within which the Taq DNA polymerase gene was inserted.用含有TaqDNA聚合酶基因的 pTaq表达质粒转化E 。
2)recombinant Taq DNA polymerase重组TaqDNA聚合酶
1.coli BL21 (DE3), and the recombinant Taq DNA polymerase was expressed by IPTG induction.coliBL21(DE3),经IPTG诱导后,重组TaqDNA聚合酶在大肠杆菌中实现了大量表达。
3)Commercial Taq DNA polymerase商品TaqDNA聚合酶
4)Enzymatic polymerization酶促聚合
1.Novel Functional Block Copolymer Combining Enzymatic Polymerization and ATRP and Its Self-Assembly Behavior Study;酶促聚合方法和ATRP方法结合制备新型功能嵌段共聚物及其自组装行为研究
英文短句/例句
1.Novel Functional Block Copolymer Combining Enzymatic Polymerization and ATRP and Its Self-Assembly Behavior Study;酶促聚合方法和ATRP方法结合制备新型功能嵌段共聚物及其自组装行为研究
2.Studies of Novel Functional Polymers Combination of Enzymatic Polymerization and ATRP;酶促与ATRP方法结合制备新型功能聚合物的研究
3.Studies on Radical Polymerization of Vinyl Monomers Mediated by Horseradish Peoxidase Redox System;辣根过氧化物酶酶促体系引发乙烯基单体聚合研究
4.Functional Polycarbonate: Enzymatic Synthesis and Modification to Polylactide;功能化聚碳酸酯的酶促合成及其对聚乳酸的改性
5.Synthesis and Enzymatic Degradation of Degradable Polyurethanes with Shape-memory Effect;可降解形状记忆聚氨酯的合成及其酶促降解研究
6.Biodegradable Polycarbonates with High Molecular Weight; Enzymatic Synthesis and Properties Improvement;高分子量生物可降解聚碳酸酯的酶促合成及改性
7.The Enzymatic Synthesis of the Dehydrodimer of Resveratrol--cis-δ-viniferin and the Study of Its Function白藜芦醇二聚体——cis-δ-viniferin的酶促合成及其功能研究
8.LIPASE-CATALYZED SYNTHESIS AND PROPERTIES OF THIOL END-FUNCTIONALIZED POLYCAPROLACTONE AND POLY(ETHYLENE GLYCOL)-b-POLYCAPROLACTONE末端巯基化聚己内酯和聚乙二醇-b-聚己内酯的酶促合成及其性质研究
9.dna dependent rna polymerase依赖dna的rna聚合酶
10.RNA-dependent DNA polymerase依赖于ＲＮＡ的ＤＮＡ聚合酶
11.deoxyribonucleic acid-dependent DNA依赖于ＤＮＡ的ＤＮＡ聚合酶
12.Studies on Enzymic Synthesis of Oligopeptides II. Thermolysin-Catalyzed Synthesis of Peptides Containing a Side Chain Free Carboxyl Group酶促合成肽的研究 Ⅱ.含羧基侧链氨基酸的肽的酶促合成
13.Extraction of glucan from the cell wall of waste beer yeast by autolysis coupled with enzymes addition啤酒废酵母酶促自溶胞壁残渣中葡聚糖的提取
14.Study on enzyme catalysis conditions of recombinant S-adenosylmethionine synthetase重组腺苷蛋氨酸合成酶酶促反应条件的研究
15.Study on the Enzymatic Synthesis of Biodiesel from Rapeseed Oil;油菜生物柴油的酶促合成新技术研究
16.Synthesis and Analysis Carnosine and the Simulacrum by Enzyme in Micro-aqueous Phase;微水相酶促肌肽类似物的合成与分析
17.Synthesis of 3-Palmityloxy Shikimic Acid Catalyzed by Lipase in Non-Aqueous Media;3-棕榈酰莽草酸的非水相酶促合成
18.Lipase-Catalyzed Synthesis and Bioactivity of Capsinoids天然辣椒素酯的酶促合成与生物活性
相关短句/例句
recombinant Taq DNA polymerase重组TaqDNA聚合酶
1.coli BL21 (DE3), and the recombinant Taq DNA polymerase was expressed by IPTG induction.coliBL21(DE3),经IPTG诱导后,重组TaqDNA聚合酶在大肠杆菌中实现了大量表达。
3)Commercial Taq DNA polymerase商品TaqDNA聚合酶
4)Enzymatic polymerization酶促聚合
1.Novel Functional Block Copolymer Combining Enzymatic Polymerization and ATRP and Its Self-Assembly Behavior Study;酶促聚合方法和ATRP方法结合制备新型功能嵌段共聚物及其自组装行为研究
5)Polyketide synthase聚酮合酶
1.Gene deletion vector pXL05 was used to disrupt oligomycin polyketide synthase (PKS) encoding genes(olmA) in Streptomyces a.本研究以产阿维菌素B和寡霉素的阿维链霉菌CZ8-73为出发菌株，构建了基因缺失载体pXL05，并将其转入CZ8-73中，通过缺失载体和染色体之间的同源双交换，对染色体上长达90kb的寡霉素聚酮合酶(PKS)基因簇(olmA)进行了缺失。
2.The recombinant Sorangium cellulosum in which the epoK gene has been inactivated by random mutagenesis and so produces only epothilone D and C,does not produce epothilone A or B,duo to an alteration in the genes coding for the epothilone polyketide synthase(PKS).重组纤维堆囊菌的epoK基因因随机诱变而失活,由于埃博霉素聚酮合酶(PKS)基因编码的改变,生产菌株只产生埃博霉素D和C,而不产生埃博霉素A和B。
3.OBJECTIVE To clone and characterize type Ⅲ polyketide synthases (PKSs) gene from Fallopia multiflora.目的克隆并表征中药材何首乌(Fallopia multiflora)中的Ⅲ型聚酮合酶(polyketide synthases,PKS)基因。
6)RNA polymeraseRNA聚合酶
1.Subcloning and expression of influenza virus RNA polymerase PB1-1 fragment;流感病毒RNA聚合酶PB1-1亚基片段的克隆及表达
2.Isolation and purification of RNA polymerase from influenza virus;流感病毒RNA聚合酶的分离与纯化(英文)
3.Stringent RNA polymerase of E. coli and its in vivo transcriptional activity;大肠杆菌严谨型RNA聚合酶的筛选及体内转录活性测定
延伸阅读
Taqdna聚合酶taq dna聚合酶是从一种水生栖热菌(thermusaquaticus)yt1株分离提取的.yt是 一种嗜热真菌，能在70～75℃生长.该菌是1969年从美国黄石国家森林公园火山温泉中分离的。该酶基因全长2496个碱基，编码832个氨基酸，酶蛋白分子为 94kda.其比活性为200000单位/mg.75～80℃时每个酶分子每秒钟可延伸约150个核苷 酸，70℃延伸率大于60个核苷酸/秒，55℃时为24个核苷酸/秒.温度过高(90℃以上) 或过低(22℃)都可影响taq dna聚合酶的活性，该酶虽然在90℃以上几乎无dna合成， 但确有良好的热稳定性，在pcr循环的高温条件下仍能保持较高的活性.在92.5℃、95℃ 、97.5℃时，pcr混合物中的taq dna聚合酶分别经130min，40min和5～6min后，仍可 保持50%的活性，实验表明.pcr反应时变性温度为95℃～20sec，50个循环后，taq dna聚合酶仍有65%的活性.taq dna聚合酶的热稳定性是该酶用于pcr反应的前提条 件，也是pcr反应能迅速发展和广泛应用的原因.taq dna聚合酶还具有逆转录活性， 其作用于类似逆转录酶.此活性温度一般为65～68℃，有mn2+存在时，其逆转录活性 更高.taqdna聚合酶是mg2+依赖性酶，该酶的催化活性对mg2+浓度非常敏感.以活性程度 很低的鲑鱼精子dna为模板，dntp的浓度为0.7～0.8mmol/l时，用不同浓度mg2+进行 pcr反应10min，测定结果为mgcl2浓度在2.0mmol/l时该酶催化活性最高，此浓度能最 大限度地激活taqdna聚合酶的活性，mg2+过高就抑制酶活性，当mgcl2浓度在 10mmol/l时可抑制40～50%的酶活性.由于mg2+能与dntp结合而影响pcr反应液中游离 的mg2+浓度，因而mgcl2的浓度在不同的反应体系中应适当调整，优化浓度.一般反应 中mg2+浓度至少应比dntp总浓度高0.5～1.0mmol/l.适当浓度的kcl能使taq dna聚合 酶的催化活性提高50～60%，其最适浓度为50mmol/l，高于75mmol/l时明显抑制该酶 的活性.
