Real-time PCR,Real-time RT-PCR
1)Real-time RT-PCRReal-time PCR
1.In real-time RT-PCR assay,Mce4E protein stimulated alveolar macrophage for 48 hours,which significantly(P<0.bovis,MbPPD)、卡介苗(Bacille Calmette Guerin,BCG)和刀豆蛋白A(con-canavalin A,ConA)分别刺激肺泡巨噬细胞48h后,real-time PCR方法检测显示,Mce4E蛋白能够促使牛肺泡巨噬细胞TNF-α和IL-6 mRNA的表达上调(P<0。
2.In real-time RT-PCR assay, Mb mce4E protein stimulated alveolar macrophage for 48 hours, which induced the expression of TNF-α and IL-6 mRNA enhanced at p<0.越来越多的研究证实mce家族蛋白与结核杆菌的毒力有关,为了探讨mce4E蛋白在牛分支杆菌致病机理中的作用,我们用重组表达的mce4E蛋白、MtbPPD、MbPPD、BCG和 ConA刺激肺泡巨噬细胞48小时后,用real-time PCR检测牛肺泡巨噬细胞TNF-α、iNO、 IL-6、IL-12细胞因子mRNA的表达量的变化,与空白对照组相比,mce4E、MtbPPD、MbPPD、 BCG、ConA均能够使巨噬细胞的TNF-α mRNA表达上调(P<0。
英文短句/例句

1.Rapid detection of canine parvovirus by Real-time PCR and PCR methodsReal-time PCR和PCR方法快速检测犬细小病毒
2.Research Progress in the Application of Real-time PCRReal-time PCR技术的应用研究进展
3.Establishment of Real-time PCR Assay for Quantitative Analysis of AlHAK1獐茅AlHAK1 Real-time PCR定量方法的建立
4.Establishment and Application of Real-Time PCR for Detection of HCMV;Real-time PCR检测HCMV的方法学建立及初步应用
5.Quantitative Assay Rhizoctonia Solani AG1-IA in Soil Via Real-time PCR;Real-time PCR用于土壤中稻纹枯病原菌的定量监测
6.Development and Clinical Trial of Fluorescence Real Time PCR to Detect Rubella Virus;Real-time PCR检测风疹病毒方法学的建立及初步应用
7.Development and Application of Real-Time PCR for Detection of Toxoplasma Gondii;Real-time PCR检测弓形虫方法学的建立及初步应用
8.Application of Real-time PCR Assay in the Early Diagnosis of Tuberculosis;Real-time PCR技术在结核病早期诊断中的应用
9.The Establishment and Applification of Multiplex Real-Time PCR for Detection of Vibrio Parahaemolyticus and Vibrio Vulnificus;副溶血弧菌和创伤弧菌双重Real-time PCR检测方法的建立及其应用
10.Development of Real-time PCR Assay for Detecting RVFV and Expression of G2 Partial Protein by E.coli;裂谷热病毒Real-time PCR检测方法的建立及其G2部分蛋白的原核表达
11.Study on the replication of porcine parvovirus virus in PK-15 cells using Real-time PCR technology利用Real-time PCR技术检测猪细小病毒在PK-15细胞增殖动态研究
12.Detection of influenza virus by Real-Time RT-PCR in Jinhua City in 2008金华市2008年流感病毒Real-Time RT-PCR检测
13.Development and Application of a Real Time PCR Approach for Quantification of Rumen Microorganisms;瘤胃微生物Real Time PCR定量方法的建立及其应用
14.Development and Application of Real Time PCR Approach for Quantification of Six Plant Viruses;六种植物病毒Real Time PCR定量方法的建立及其应用
15.Detection Establishment and Application of SYBR Green Real-Time Polymerase Chain Reaction for Porcine Parvovirus猪细小病毒SYBR Green real-time PCR检测方法的建立及应用
16.REAL-TIME RT-PCR DETECTION AND CLINICAL ANALYSIS FOR YOUNG CHILDREN A/H1N1幼儿甲型H1N1流感的Real-time RT-PCR检测及临床分析
17.Development of one step Real-time RT-PCR method for detection of Hendra virusHendra病毒一步法Real-time RT-PCR检测方法的建立
18.Development of a real-time PCR assay based on SYBR GreenⅠ for detection of porcine encephalomyocarditis virus猪脑心肌炎病毒SYBR GreenⅠ real-time PCR检测方法的建立
相关短句/例句

nested-real-time PCRnested-real-time-PCR
3)Rreal-time fluorogenic PCRReal-time荧光PCR
4)Multiplex Real-Time PCR双重Real-time PCR
1.The Establishment and Applification of Multiplex Real-Time PCR for Detection of Vibrio Parahaemolyticus and Vibrio Vulnificus;副溶血弧菌和创伤弧菌双重Real-time PCR检测方法的建立及其应用
5)TaqMan real-time RT-PCRTaqMan real-time RT-PCR方法
6)Real-time RT-PCRReal-timeRT-PCR
延伸阅读

ageing time分子式:CAS号:性质:塑料或其他高分子材料经过天然曝露试验、人工耐候试验、热老化试验后,某些性能降到规定程度所需要的时间。通常以经过的年、月、日或小时表示。在户外曝露试验中也有用试样表面受到大于或等于3.44J/(cm2·min)辐射强度太阳照射的时间—太阳小时来表示。