1)allele specific amplification等位基因特异性扩增
1.[Methods] A total of 100 healthy Liaoning Hans were studied by the use of PCR and allele specific amplification (ASA) techniques.方法用聚合酶链反应及等位基因特异性扩增技术对100名辽宁地区汉族人的DRD4基因启动子区3个多态性进行了检测,并与其他人群做了比较。
英文短句/例句
1.Multiplex SNP typing using monotube allele-specific amplification单管等位基因特异性扩增法同时测定多重单核苷酸多态性
2.Adapter-ligation Mediated Allele-specific Amplification (ALM-ASA) for Multiplex SNP Genotyping of LRRK2应用基于适配器连接介导的等位基因特异性扩增法检测LRRK2基因的单核苷酸多态性
3.Microchip electrophoresis coupled with multiplex allele-specific amplification for typing multiple single nucleotide polymorphisms(SNPs) simultaneously多重等位基因特异性扩增——微流控芯片电泳法同时测定多个单核苷酸多态性位点
4.Study on polymorphism of human eighth complement component(C8A)by using allele specific amplification method;用等位基因特异扩增技术检测人类补体第八成份C8A DNA多态性的研究
5.A technique for ABO genotyping by consumed allele-specific primer analysis;ABO基因分型的等位基因特异性引物消耗法
6.Preliminary Research on Gold Nanoparticles Enhancing the Specificity of PCR Amplification of Low Copy Gene from the Complicated System纳米金增强复杂体系中PCR扩增低拷贝基因的反应特异性初步研究
7.Development of PCR for detection of Mycobacterium bovis specific gene牛分枝杆菌特异性基因的PCR扩增及二联PCR反应的建立
8.Analysis of MDR1 gene-3435C/T polymorphisms by allele-specific polymerase chain reaction等位基因特异性聚合酶链反应检验多药耐药基因的多态性
9.A Simple and Rapid Modified -New Method for SNP Typing by Fragment Length Discrepant Allele Specific PCR;片段长度差异等位基因特异性PCR—一种改良的SNP分型新方法
10.Rapid Detection of Isoniazid Resistant Mycobacterium Tuberculosisby Allele Specific Multiplex PCR等位基因特异多重PCR快速检测结核分枝杆菌异烟肼耐药性研究
11.Detection of the CETP gene mutation by AS-PCR应用等位基因特异性PCR检测胆固醇酯转运蛋白基因突变
12.allele specific oligonucleotide等位基因特异的寡核苷酸
13.A New Method for SNP Typing Based on Allele Specific PCR;基于等位基因特异性PCR原理建立的SNP分型新方法
14.Optimizing the conditions for in vitro expansion of HBV protein epitope-specific CTLs乙肝病毒蛋白抗原表位特异性CTL体外扩增条件优化
15.A Convenient Method for Condon Adjustment by Using High-fidelity DNA Polymerase Amplification Property利用高保真酶扩增特性调整基因读码框的方法
16.Analysis of different resistant materials with this marker proved its specificity for the M4 gene.对不同抗源材料的扩增分析表明该标记是M4基因的特异标记。
17.Cloning and Sequence Analysis of Genome-P Specific DNA Fragments in Agropyron cristatum Using ISSR冰草P基因组ISSR扩增特异DNA片段的克隆及序列分析
18.Assaying Single Nucleotide Polymorphism of High Pigment Genes hp1 and hp2 Based on Allele-specific PCR in Tomato利用等位基因特异性PCR技术检测番茄高色素基因hp1和hp2的单核苷酸多态性
相关短句/例句
allele-specific amplification等位基因特异性扩增
1.Multiplex SNP typing using monotube allele-specific amplification单管等位基因特异性扩增法同时测定多重单核苷酸多态性
2.Microchip electrophoresis coupled with multiplex allele-specific amplification for typing multiple single nucleotide polymorphisms(SNPs) simultaneously多重等位基因特异性扩增——微流控芯片电泳法同时测定多个单核苷酸多态性位点
3.An accurate and effective method was developed for rapidly genotyping in a single tube, which was based on di-allele-specific amplification with artificially modified primers.该方法在等位基因特异性扩增法的基础上,在内引物3′端引入了一个人工错配 碱基并在5′端附加一段“尾巴”作为引物的锚定部分,使扩增反应的特异性得到了提高。
3)Allele-specific amplification等位基因特异扩增法
1.Objective:To establish Allele-specific amplification(ASA-PCR)for study the distribution of MDR1 polymorphism in transplant patients.目的:建立等位基因特异扩增法(Allele-Specific Amplification,ASA-PCR)研究移植患者多药耐药基因(multi-drug re- sistance gene,MDR1)多态性。
4)Allele specific amplification等位基因特异扩增
1.Allele specific amplification (ASA) is a low-cost, robust technique that can be utilized to discriminate between the all.本试验通过等位基因特异扩增(ASA)方法对9个香稻和3个非香稻品种(品系),以及3个香稻/非香稻组合的F1的香味基因,进行快速检测。
5)allele specific amplification等位基因特异扩增法
1.An allele specific amplification based PCR method was built up for the detection of CYP2D6T allele which is a 1 795 T deletion on CYP2D6 gene and results in a function deficient enzyme.利用等位基因特异扩增法的基本原理,建立了测定CYP2D6T的方法。
6)allele specific amplification (ASA PCR)等位基因特异扩增法(ASAPCR)
延伸阅读
基因扩增分子式:CAS号:性质:又称基因放大。是指在一定条件下,把特定的基因(群)加以大量复制的现象。(1)高等生物为了表达在特定发育期中的某一特定性状,决定该种性状的基因数便会大幅度增加。如非洲爪蟾的卵母细胞中的rRNA基因,比体细胞的数量约大1500倍。(2)在动物的培养细胞中,对双氢叶酸还原酶(DHFR)的抑制剂甲氨蝶呤具有耐药性的细胞,已知其DHFR基因增加达1000个拷贝,已使用于对基因扩增机制的分析等。(3)在细菌中,已知有质粒数(拷贝数)增加的现象。基因扩增的这一形式,可被用来增加目的基因的产量等(基因剂量效应)。
