1)IFN-γ geneIFN-γ基因
1.Cloning,phylogenetic evolution and expression of rabbit IFN-γ gene;兔IFN-γ基因的克隆、遗传进化分析及其表达
2.One pair of primers was designed according to duck interferon-gamma(IFN-γ)gene sequences published in GenBank,which was used to amplify the IFN-γ gene from total RNA extracted directly from Gushi duck splenocyte without induction of PHA by reverse transcription-polymerase chain reaction(RT-PCR).序列比较分析发现,克隆的固始鸭IFN-γ基因序列与GenBank中4条鸭IFN-γ基因序列中的3条序列(AF087134,AF100929,AJ012254)完全一致,而与另一条北京鸭核苷酸同源性为99。
3.From the total RNA,an IFN-γ gene of 435bp was amplified by RT-PCR.以此总RNA为模板,采用RT-PCR技术扩增到了435 bp的IFN-γ基因。
英文短句/例句
1.Establishment of a real-time fluorescent quantitative RT-PCR assay for detection of chicken IFN-α,IFN-β and IFN-γ genes鸡IFN-α和IFN-β及IFN-γ基因实时荧光定量RT-PCR检测方法的建立
2.Cloning and Prokaryotic Expression of Chicken s IFN-γ Gene;鸡IFN-γ基因的克隆及原核表达
3.Research of Gene Therapy for Colon Cancer with Interferon-gamma (IFN-γ);IFN-γ基因治疗大肠癌的实验研究
4.Cloning and Prokaryotic Expression of Chicken IFN-γ Gene;鸡IFN-γ基因的克隆与原核表达
5.Expression of Ailuropoda melanoleuca qinlingensis interferon-gamma and bioactivity analysis of the expressed product秦岭大熊猫IFN-γ基因的表达及其生物活性分析
6.Chicken IFN-γ Gene Cloning and Expression and the Study Progress of Anti-coccidia Effect of IFN-γ鸡IFN-γ基因克隆表达及其抗球虫研究进展
7.Anti-tumor Effects of Exosomes Derived from Tumor Cells Modified by IFN-γ GeneIFN-γ基因修饰的肿瘤细胞来源exosomes的抗肿瘤效应
8.Molecular Cloning of Chicken IL-2、IL-15、IL-18 and IFN-γ and the Construction of Eukaruotic Expression Plamids and Their Adjuvant Effects on Vaccines;四川山地乌骨鸡IL-2、15、18、IFN-γ基因克隆测序及分子佐剂效应研究
9.Relationship of IFN-γ Gene Polymorphism with Susceptibility to Intrauterine HBV Infection;IFN-γ基因多态性与宫内HBV感染易感性关系的研究
10.Relationship of IFN-γ Polymorphism with Susceptibility of Aplastic Anemia;IFN-γ基因多态性与再生障碍性贫血易感性关系的研究
11.Association of Interferon-γ Gene Haplotype in the Chinese Population with Hepatitis B Virus Infection;中国汉族人群IFN-γ基因单倍型与HBV感染关联性研究
12.Study on the Single Nucleotide Polymorphism of Interferon-gamma Gene +874 Site Associated with Breast Cancer;IFN-γ基因+874位点单核苷酸多态性与乳腺癌的相关性研究
13.Experimental Study on Antitumoral Efficiency of IFN-γ Gene Transfection by Ultrasound Mediated SonoVue Microbubble Destruction in Vitro and in Vivo;超声介导SonoVue微泡转IFN-γ基因抗肿瘤作用的实验研究
14.Gene Cloning、Expression and Bioactivation Detection of Ailuropoda Melanoleuca Qinlingensis IFN-γ秦岭大熊猫IFN-γ基因的克隆、表达及免疫活性研究
15.Cloning, Prokaryotic Expression of Ovine Interferon Gamma Gene and Study on Antiviral Activity of Its Recombinant Protein;绵羊γ干扰素(IFN-γ)基因的克隆、表达与抗病毒活性初步研究
16.Up-regulation of Fas Is Related to Apoptosis of HeLa Cells Induced by Co-immobilized TNF-α/ IFN-γFas基因在共固定化细胞因子TNF-α/IFN-γ诱导HeLa细胞凋亡中的表达
17.Cloning, Expression of BoIFN-α/γ Genes and Application of Recombinant Proteins;牛IFN-α/γ基因的克隆、表达及重组蛋白的应用研究
18.Construction, Expression and Identification of sflk1-IFN-γ Fusion Protein Encoding Recombinant Plasmid;sflk1-IFN-γ融合蛋白基因重组质粒的构建、表达及生物学活性鉴定
相关短句/例句
IFN-γgeneIFN-γ基因
1.After the total RNA was isolated from the lymphocytes, the porcine IFN-γgene was amplified by reverse transcription polymerase chain reaction (RT-PCR).为研究猪γ-干扰素在抗御传染性疾病和肿瘤生物治疗中的免疫增强作用,本实验以ConA刺激成华猪外周血淋巴细胞提取激活淋细胞的mRNA,设计合成特异的引物序列,应用反转录-聚合酶链式反应(RT-PCR)扩增和克隆成华猪IFN-γ基因并进行序列测定。
2.The result indicates that the cloned gene was acomplete PoIFN-γgene.将目的片断克隆到pMD18-T载体上,酶切鉴定及序列测定结果表明为IFN-γ基因。
3)goat IFN-γ gene山羊IFN-γ基因
1.We constructed the recombinant transfer vector pTK-VP-IFNγ by inserting the goat IFN-γ gene into pTK-VP1 which contains the FMDV vp1 gene,The pTK-VP-IFNγ vector was purified and transfected by lipofection into bovine testicle cells that was infected with rAV41-LacZ expressing Escherichia coli β-Galactosidase (LacZ).将山羊IFN-γ基因插入到含有口蹄疫病毒(FMDV)vp1基因的山羊痘病毒(GPV)转移载体PTK-vp1中,获得含有FMDVvp1基因和山羊IFN-γ基因的重组转移载体pTK-vp-IFNγ,以本实验室构建的表达β-半乳糖苷酶基因(LacZ)的山羊痘重组病毒rAV41-LacZ为亲本毒,与转移载体pTK-vp-IFNγ共同转染犊牛睾丸细胞进行同源重组,通过7轮反向蓝白斑筛选,产生重组山羊痘病毒rAV41-VP-IFNγ,通过PCR和间接免疫荧光实验证实获得了能够稳定表达FMDVvp1和山羊IFN-γ的重组山羊痘病毒。
4)IFN-α2b geneIFN-α2b基因
5)IFN-a geneIFN-a基因
1.Construction of recombinant adenoviral vector co-expressing G gene of RV and IFN-a gene;狂犬病毒CVS株G基因和IFN-a基因共表达重组腺病毒载体的构建
6)Interferon-tau geneIFN-τ基因
延伸阅读
IFN-γ分子式:CAS号:性质:又称免疫干扰素。由人体T淋巴细胞在受到病毒感染的刺激时产生。或在体外的实验系统中,用各种诱生剂,包括来自葡萄球菌的蛋白A(外毒素)诱导产生。分子量20 000~25 000,为糖蛋白,具有多种生物活性。pH值5以下不稳定,热稳定性差,免疫功能强。已可用基因操作法大量生产。
