1)enzyme digestion酶消化法
1.METHODS:Freshly extracted human permanent teeth were collected and sectioned under cooling ECS solution and then the odontoblasts were isolated through enzyme digestion.方法:选取新鲜拔除的健康恒牙,利用牙科专用慢速切锯制备牙齿切片,采用酶消化法分离出完整的人成牙本质细胞,利用RT-PCR技术直接从人成牙本质细胞中克隆L型钙离子通道α1亚基D亚型特异性基因片段,将其亚克隆入pGM-T载体进行序列测定。
2.Methods The sertoli cells in rats testicular were obtained through multiple enzyme digestion and these cells were cultivated in vitro to maintain their favorable activities.方法利用多重酶消化法,获取睾丸Sertoli细胞,进行体外原代培养以维持其良好的活性。
3.Results:The HOBs purified by enzyme digestion in vitro had a normal function showing synthesized alkaline phosphatase(ALP)and forming calcified nodules as observed in vivo,and significant difference in optical density(OD490) was found between morphine group with different concentrations and the control group(P<0.方法:用胰酶-胶原酶消化法从正常女性松质骨中分离培养成骨细胞,用茜素红染色法、改良Gomonri钙钴法鉴定成骨细胞;制备不同浓度的吗啡及其拮抗剂纳洛酮,作用于体外培养的成人正常成骨细胞上,通过四甲基偶氮唑盐(MTT)比色法检测吗啡对其的影响;通过RT-PCR法半定量分析不同浓度的吗啡及其拮抗剂纳洛酮对成骨细胞骨钙素(BGP mRNA)表达的影响。
英文短句/例句
1.Comparative study of strong acid digestion method and trypsin digestion method for extracting diatom;强酸消化法和胰蛋白酶消化法检验腐败脏器硅藻的比较研究
2.Methods The esophageal carcinoma cell line CSEC was established by collagenase digestion methods.方法采用胶原酶消化法建立人食管癌细胞系CSEC。
3.Mammalian skeleton specimen preparation using proteolytic enzyme(alcalase)detergent兽类骨骼标本制作新方法:碱性蛋白酶消化法
4.Isolation and culture of pulmonary artery smooth muscle cells of broiler by the enzymatic digestion method酶消化法分离培养肉鸡肺动脉平滑肌细胞
5.Repeated Pancreatic Enzyme-degisting Method for Culturing Muller Cells of SD Rats反复胰蛋白酶消化法培养SD大鼠视网膜Muller细胞
6.Conclusion The enzyme digestion procedure is a stable and reliable method to obtain bovine retinal pericytes.结论:酶消化法原代培养牛视网膜周细胞是一种稳定、可靠的方法。
7.Evaluation of the Utilizing Effect of Protein in Glucose-treated Soybean Meal by Nylon Bag Technique and Enzymatic Technique用尼龙袋法及酶消化法对葡萄糖处理豆粕蛋白利用效果评价研究
8.Culture and identification of mouse osteoblasts by modified enzymatic digestion in vitro优化酶消化法分离培养及鉴定小鼠前体成骨细胞
9.Islets were isolated by collagenase digestion and were transplanted under the left kidney capsule.采用胶原酶消化法分离胰岛并移植到左肾被膜下。
10.Explant combined with trypsin-digestion for culture of endothelial cells from infantile hemangiomas组织块结合酶消化法培养婴幼儿血管瘤内皮细胞
11.enzymatic removal酶催[化] 消除[法]
12.DIRECT ENZYMATIC SEQUENCING OF RNA核糖核酸的酶消化方法直接顺序分析
13.Study on the Digestibility of Protein of Feeds with Enzyme by a Pepsin-Pancreatin in Vitro Method;胃蛋白酶—胰酶两步法体外评定加酶饲料蛋白质消化率的研究
14.Optimization of technology for production of maize slowly digestible starch by enzymatic method酶法制备玉米缓慢消化淀粉的工艺条件优化
15.Digestive Enzyme in Turbot Scophthalmus Maximus L.;大菱鲆主要消化酶—蛋白酶、脂肪酶、淀粉酶的研究
16.Estimation of the Digestible Energy Values of Soybean Protein Supplement with Chemical and Enzymic Method;用化学法和酶法估测大豆蛋白类饲料消化能的研究
17.Digestibility of Protein Ingredients by Commercial Enzyme and Chinese Drum Miichthys miiuy Digestive Enzyme in vitro鱼免消化酶与商品酶离体消化蛋白原料的研究
18.Study on the Histology and Enzyme in the Digestive Tract of Ophicephalus Argus;乌鳢消化系统组织学及消化酶的研究
相关短句/例句
One enzyme digestion method单酶消化法
3)Cold enzyme digestion冷酶消化法
4)Disaggregating by double enzymes双酶消化法
5)Trypsin-digestion method胰酶消化法
6)Pepsin digestion method胃蛋白酶消化法
延伸阅读
胨[番木瓜蛋白酶消化大豆粉制备]CAS: 91079-46-8 中文名称: 胨[番木瓜蛋白酶消化大豆粉制备] 英文名称: Peptone from soya bean flour papain-digested
