葡聚糖内切酶,endoglucanase
1)endoglucanase葡聚糖内切酶
1.The cDNA genes encoding endoglucanases Ⅰ and Ⅲ were isolated by PCR,and then they were cloned into a yeast vector to construct the cellulases-producing Saccharomyces cerevisiae engineering strains.PCR扩增葡聚糖内切酶(EG)和葡聚糖内切酶(EG)的cDNA基因,将其克隆到酵母载体中,构建产生纤维素酶的酿酒酵母工程菌。
2.β-1,4-endoglucanase encoding gene was cloned from Bacillus pumilus H9 producing alkaline cellulase,with its sequence and domain being also analyzed.从一株产碱性纤维素酶的短小芽孢杆菌菌株H9中,克隆到了编码葡聚糖内切酶的基因,对其基因序列及酶的结构域进行了分析预测,同时将该酶的基因构建于大肠杆菌表达载体pET20b中,获得重组表达载体pET20b-EglA,转化至大肠杆菌菌株BL21(DE3)中进行表达,并进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳检测。
3.Most of filamentous fungi produces cellulase,especially the Trichoderma spp which produces endoglucanases and cellobiohydrolases in high level,and Aspergillus which produces 1,4-β-D-glucosidases in high level.葡聚糖内切酶(endo-1,4-,β-D-glucanases,简称EG);(3)。
英文短句/例句

1.Gene Cloning and Expression of Endoglucanase from Trichoderma Viride;绿色木霉葡聚糖内切酶基因的克隆和表达研究
2.Expression and Molecule Evolution of the Gene of Endoglucanase Ⅰ in Escherichia Coli;绿色木霉葡聚糖内切酶Ⅰ基因在大肠杆菌中的表达与分子改造
3.The Studies on the Endo-β-1,4-glucanases from the Mollusca, Ampullaria Crosseans;福寿螺(Ampullaria crossean)内切-β-1,4葡聚糖酶的研究
4.Study on Endoglucanase of Marine Bacterium Cellulophaga sp. QY201海洋细菌Cellulophaga sp. QY201的内切葡聚糖酶研究
5.Research Progress on Plant Endo-β-1,4-Glucanase Genes植物内切β-1,4-葡聚糖酶基因研究进展
6.Cloning and Expression of Genes Encoding β-Mannanase and Endo-β-1,3-Glucananse;β-甘露聚糖酶和内切β-1,3-葡聚糖酶的基因克隆与表达
7.Study of Molecular Directional Evolution of Endoglucanase of Trichoderma. Spp.;木霉内切葡聚糖苷酶(EG)分子定向进化的研究
8.Research on Cloning and Expression of Endoglucanase Gene from Trichoderma Longibrachiatum长梗木霉内切葡聚糖酶基因的克隆与表达研究
9.Over-expression of Endoglucanase Gene in Pichia pastoris内切葡聚糖酶基因在毕赤酵母中高效表达研究
10.Studies on Liquid Fermentation of Cellulases and Purification of an Endocellulase from Chaetomium Thermophile;嗜热毛壳菌纤维素酶液体发酵条件和内切β-葡聚糖酶的分离纯化
11.Cloning of Chitinase and Endoglucanase Gene from Bacillus spp. and Construction of Recombinant Bacillus Isolates;几丁质酶和内切葡聚糖酶编码基因的克隆及重组芽孢杆菌的构建
12.Purification of β-endoglucanase in T. reesei QM9414 and its enzymological study瑞氏木霉β-内切葡聚糖酶的纯化及其部分酶学性质研究
13.Production and Characterization of Thermotolerant Endoglucanase from Streptomyces xylophagus KX6蚀木链霉菌KX6耐热内切葡聚糖酶的产生及酶学性质研究
14.Study on the Properties and Purification of Endo-β-1, 4-Glucanase from Odontotermes Formosanus(Isoptera:Termitidae);黑翅土白蚁内切-β-1,4-葡聚糖酶分离纯化及其特性研究
15.Genetic Transformation of Cotton with Endo-1,4-β-glucanase Gene and Regeneration of Transgenic Plants;内切-1,4-β-葡聚糖酶基因转化棉花及转基因植株的再生研究
16.Cloning and Sequence Analysis of the Membrane-anchored Endo -1, 4-β-Glucanase Gene from Gossypium Hirsutum L.;棉花与膜结合的内切-1,4-β-葡聚糖酶基因的克隆及序列特征分析
17.Cloning and Expression of an Endoglucanase Gene from Bacillus Thermoliquefaciens;嗜热液化芽孢杆菌内切葡聚糖酶基因的克隆和表达
18.Expression of Endoglucanase Gene from Bacillus Subtilis C-36 in Escherichia Coli;枯草芽孢杆菌C-36内切葡聚糖酶基因在大肠杆菌中的表达
相关短句/例句

endo-β-1,3-glucanse内切-β1,3-葡聚糖酶
3)endo β glucanase Ⅲ葡聚糖内切酶Ⅲ
1.To study the construction of yeast bioengineering strain which can degrade cellulosic waste, an endo β glucanase Ⅲ(EGⅢ) cDNA gene of Trichoderma viride AS3 3711 was isolated with RT PCR protocol.为研究构建可降解纤维类固体废弃物的工程菌 ,采用RT PCR方法克隆到绿色木霉 (Trichodermaviride)AS3 371 1的葡聚糖内切酶Ⅲ (EGⅢ )的cDNA基因 ,测序后构建到酿酒酵母 (Saccharomycescerevisiae)诱导型表达载体pYES2上 ,用正交实验对超声波辅助酵母转化系统进行了优化 。
4)endoglucanase内切葡聚糖酶
1.Expression of endoglucanase gene in Bacillus Megaterium and characterization of recombinant enzyme;内切葡聚糖酶基因在巨大芽孢杆菌中的表达及其酶学性质研究
2.Role of Tryptophan Residue in Endoglucanase from Trichoderma pseudokoningii S 38;色氨酸残基在内切葡聚糖酶分子中的作用
3.The Effect of Amphopathics for Endoglucanase against the inhibitionof Ethanol;两亲分子解除乙醇对内切葡聚糖酶的抑制作用
5)Endoglucanase内切葡聚糖苷酶
1.Endoglucanase CelG gene and its signal peptide sequences of Clostridium thermocellum were cloned and expressed in this paper.对热纤维素梭菌Clostridium thermocellum内切葡聚糖苷酶基因CelG及其信号肽序列进行了克隆及表达研究。
2.Purification and Intrinsic Fluorescence Properties of Endoglucanases from Trichoderma pseudokiningii S 38;利用离子交换和分子筛层析技术从拟康氏木霉S-38固态发酵液中提纯了两个内切葡聚糖苷酶组分。
6)β-endoglucanaseβ-内切葡聚糖酶
1.Expression of β-endoglucanase Gene from Vibrio fischeri in Escherichia coli and Its Enzymological Characterization;费氏弧菌β-内切葡聚糖酶基因在大肠杆菌中的表达及酶学分析
2.Purification of β-endoglucanase in T. reesei QM9414 and its enzymological study瑞氏木霉β-内切葡聚糖酶的纯化及其部分酶学性质研究
延伸阅读

内型-1,3(4)-β葡聚糖酶CAS:62213-14-3 中文名称: 内型-1,3(4)-β葡聚糖酶 英文名称: endo-1,3(4)-.beta.-Glucanase;laminarinase from mollusk