改良消减杂交技术,modified subtractive hybridization英语短句,例句大全

改良消减杂交技术,modified subtractive hybridization
1)modified subtractive hybridization改良消减杂交技术
1.Method:By modified subtractive hybridization technique, total RNA sample of Peripheral venous blood extracted from the patient of congenital hypoplasia of teeth roots and normal brother were subtracted to screen the differentially expressed cDNA of candidate virulence gene related with this disease.方法 :采用改良消减杂交技术 ,以先天性牙根发育不全疾病患儿与其正常兄弟外周静脉血的总mRNA为对比材料 ,筛选出候选相关致病基因差异表达的cDNA ,将其与 pGEM -T载体进行T/A连接构建文库 ,然后转化高效感受态大肠杆菌进行文库扩增 ,随机挑取 10 0个白色克隆进行酶切鉴定。
2.Method: By modified subtractive hybridization technique, we collected one boy with congenital hypoplasia of teeth roots and his healthy brother to obtain cDNA library of blood, then we constructed subtractive cDNA library.方法:采用改良消减杂交技术,以先天性牙根发育不良患儿及其正常兄弟为研究对象,制备血液基因表达cDNA文库,构建两者的差异表达文库,采用反向点杂交技术剔除假性克隆,挑取部分克隆进行定性测序。
2)improved subtractive hybridization改良消减杂交法
1.In order to study the mechanism of hyperplasy and invasiveness of fibroblast\|like synovial cells(FLS)of rheumatoid arthritis(RA)patients,an improved subtractive hybridization was used to screen for the genes which expression was increased in RA FLS compared with FLS of osteoarthritis (OA) patients.为了研究类风湿性关节炎 (rheumatoid arthritis,RA)滑膜细胞 (fibroblast- like synovialcells,FLS)过度增殖和破坏软骨的分子机理 ,利用改良消减杂交法以骨性关节炎 (osteoarthritis,OA)病人滑膜细胞为对照 ,筛选 RA滑膜细胞中的高表达基因。
3)Suppression subtractive hybridization抑制消减杂交技术
1.Suppression subtractive hybridization in the cloning of gene fragments in relation to lung cancer metastasis;抑制消减杂交技术克隆肺癌转移调控基因
2.Research on cloning gene associated with lung cancer metastasis using suppression subtractive hybridization;抑制消减杂交技术在肿瘤转移调控研究中的应用
3.Suppression subtractive hybridization(SSH) is a good and highly effective method for gene cloning,which posses some character,such as convenient operation,high specificity,low background,good replication etc,and it can separate lower specific fragment.抑制消减杂交技术(suppressionsubtractivehybridization,SSH)是基因克隆的新方法,具有简便易行、特异性高、背景低、重复性好、能分离出丰度较低的特异性片段等特点。

英文短句/例句

1.Isolate the Genes Related to the Colorectal Carcinoma with Suppression Subtractive Hybridization;应用抑制消减杂交技术筛选大肠癌相关基因
2.Isolation of the Genes Related to Breast Cancer by Suppression Subtractive Hybridization;利用抑制消减杂交技术筛选乳腺癌相关基因
3.Application of Suppression Subtractive Hybridization in Cucurbits and Vegetables抑制消减杂交技术在瓜菜作物研究中的应用
4.An Experimental Study on the Screening of Lung Cancer Metastatic-related Genes by Suppression Subtractive Hybridization;抑制消减杂交技术筛选肺癌转移相关基因的实验研究
5.Screening and Cloning Tail-regeneration Related Genes of the Lizerd (Eremias Multiocellata) by Suppression Subtractive Hybridization;应用抑制消减杂交技术筛选与密点麻晰断尾再生相关的基因
6.Isolation of Fertility Restoration-related ESTs in Pepper Cytoplasmic Male Sterility Lines Using SSH利用抑制消减杂交技术分离辣椒细胞质雄性不育育性恢复相关EST
7.Comparison of Gene Expression Profiling between Retinal Ganglion-like Cells with Bone Mesenchymal Stem Cells by Suppression Subtractive Hybridization Analysis;抑制消减杂交技术筛选骨髓间充质干细胞诱导分化前后差异表达基因
8.Identification of Differently Expressed Genes in Eosinophils of Asthma Suing Suppression Subtractive Hybridization;应用抑制消减杂交技术研究外周血嗜酸细胞支气管哮喘相关基因的差异表达
9.Screening and Identification of Genes Transactived by p7TP3 Spliced Variant 1 by Suppression Subtractive Hybridization;应用抑制性消减杂交技术筛选p7TP3剪切体1反式激活基因
10.Study on Differential Expression Genes of Caski Cell Lines Induced by Exposing to the Space Environment by Suppression Subtractive Hybridization;利用抑制性消减杂交技术筛选太空诱变宫颈癌细胞差异表达基因
11.Study of Salt Tolerance Mechanism of Puccinellia Tenuiflora Using Suppression Subtractive Hybridzation and cDNA Microarray;抑制性消减杂交和cDNA微阵列技术研究星星草耐盐机理
12.Seperation of cDNA Fragments Related to Waterlogging Tolerance in Maize Roots Using Suppression Subtractive Hybridization and cDNA Microarray;利用抑制消减杂交和cDNA芯片技术筛选玉米耐渍相关的cDNA片段
13.Cloning of Metastasis-related Genes Expressed in Renal Cell Carcinoma by Suppression Subtractive Hybridization;抑制性消减杂交技术克隆肾细胞癌转移相关基因
14.Screening the Metastasis Functional Genes by Combining Suppression Subtractive Hybridization with cDNA Microarray;联合抑制消减杂交及基因芯片技术筛选肿瘤转移效应基因
15.Screening and cloning of the PS1TP5 transactivation related genes by suppression subtractive hybridization应用抑制性消减杂交技术克隆和筛选PS1TP5反式激活相关基因
16.Cloning and screening of transregulated genes of NS5ABP37 by SSH应用抑制性消减杂交技术筛选NS5ABP37的反式调节基因
17.Screening of Environment Factors Responsive Genes Via Suppressive Subtractive Hybridization Technique from Alexandrium catenella应用抑制性消减杂交技术筛选链状亚历山大藻环境条件响应基因
18.Identification of differentially expressed genes in rat asthma model by suppression subtractive hybridization technology利用抑制性消减杂交技术筛选大鼠哮喘相关基因

相关短句/例句

improved subtractive hybridization改良消减杂交法
1.In order to study the mechanism of hyperplasy and invasiveness of fibroblast\|like synovial cells(FLS)of rheumatoid arthritis(RA)patients,an improved subtractive hybridization was used to screen for the genes which expression was increased in RA FLS compared with FLS of osteoarthritis (OA) patients.为了研究类风湿性关节炎 (rheumatoid arthritis,RA)滑膜细胞 (fibroblast- like synovialcells,FLS)过度增殖和破坏软骨的分子机理 ,利用改良消减杂交法以骨性关节炎 (osteoarthritis,OA)病人滑膜细胞为对照 ,筛选 RA滑膜细胞中的高表达基因。
3)Suppression subtractive hybridization抑制消减杂交技术
1.Suppression subtractive hybridization in the cloning of gene fragments in relation to lung cancer metastasis;抑制消减杂交技术克隆肺癌转移调控基因
2.Research on cloning gene associated with lung cancer metastasis using suppression subtractive hybridization;抑制消减杂交技术在肿瘤转移调控研究中的应用
3.Suppression subtractive hybridization(SSH) is a good and highly effective method for gene cloning,which posses some character,such as convenient operation,high specificity,low background,good replication etc,and it can separate lower specific fragment.抑制消减杂交技术(suppressionsubtractivehybridization,SSH)是基因克隆的新方法,具有简便易行、特异性高、背景低、重复性好、能分离出丰度较低的特异性片段等特点。
4)suppression subtractive hybridization抑制性消减杂交技术
1.Identification of genes associated with human osteosarcoma metastasis suppression using suppression subtractive hybridization;应用抑制性消减杂交技术鉴定肿瘤转移抑制相关基因
2.Objective To screen the genes differentially expressed in human renal cell carcinoma (RCC) cell lines using suppression subtractive hybridization (SSH).目的应用抑制性消减杂交技术构建人肾癌差异表达基因文库并进行差异表达基因筛选。
3.1(-) transfected cells as the control to perform suppression subtractive hybridization (SSH).1(-)空载体分别转染肝母细胞瘤HepG2细胞系,用抑制性消减杂交技术筛选pcDNA3。
5)Suppression subtractive hybridization(SSH)抑制消减杂交技术(SSH)
6)Suppression subtracted hybridization抑制消减杂交术