pGL3-Basic荧光素酶报告基因载体,pGL3-Basic luciferase reporter vector
1)pGL3-Basic luciferase reporter vectorpGL3-Basic荧光素酶报告基因载体
2)Firefly luciferase reporter gene(fluc) vector荧光素酶报告基因载体
英文短句/例句

1.Construction of luciferase reporter gene plasmid containing hBD-2 and mutant correlated with Crohn's diseaseβ-防御素-2荧光素酶报告基因载体及克罗恩突变体的构建
2.Construction and analysis of the luciferase reporter vectors of human SMYD3 gene promoter人SMYD3基因启动子荧光素酶报告载体的构建与分析
3.The Construction of Luciferase Reporter Gene Vectors Containing Different Haplotypes DNA of Human PLUNC Gene Promoter RegionPLUNC基因启动子区荧光素酶报告载体的构建与鉴定
4.Construction of Recombinant Retroviral Vector Containing Firefly Luciferase Reporter Gene萤火虫荧光素酶报告基因重组逆转录病毒载体的构建及鉴定
5.Construction of Dual-Luciferase reporter plasmid assay system for human NOX1 geneNOX1基因荧光素酶报告基因系统的建立
6.Construction and identification of luciferase reporter gene vector containing HMGB1 promoterHMGB1启动子荧光素酶报告基因的构建及鉴定
7.Creation and Identification of Luciferase Reporter Gene Containing Human Bax Promotor人Bax启动子荧光素酶报告基因的构建和鉴定
8.Construction and identification of human Puma promotor luciferase report gene vector人Puma启动子荧光素酶报告基因的构建和鉴定
9.Molecular cloning and constructing luciferase report gene vector for the gene promoter of CCDC72,a novel modulator for hair follicule developement调控毛囊生长CCDC72基因启动子克隆及萤光素酶报告基因载体的构建
10.Construction and expression of red fluorescent protein reporter gene vector for human SOD1人铜锌超氧化物歧化酶基因启动子驱动的红色荧光蛋白报告基因载体的构建及表达
11.Construction and identification of a luciferase reporter vector containing human CXCR4 promoter人CXCR4基因启动子萤光素酶报告载体的构建及鉴定
12.Construction and expression of red fluorescent protein reporter gene vector containing human eNOS promoter人内皮细胞一氧化氮合酶红色荧光蛋白报告基因载体的构建与表达
13.Construction and functional identification of red fluorescent protein reporter plasmid for human catalase gene人CAT启动子红色荧光蛋白报告基因载体的构建及功能鉴定
14.Construction of a luciferase reporter vector with resistance of neomycin带新霉素抗性的萤光素酶报告载体的构建
15.Construction of red fluorescent protein reporter gene vector containing HMGB1 promotor and its activity under mechanical stretchHMGB1启动子红色荧光蛋白报告基因载体的构建及在机械牵张诱导下的活性检测
16.Viviperception of Transgenic Human Embryonic Stem Cells Labeled by Luciferase荧光素酶标记转基因人胚胎干细胞的活体观察
17.Establishment of a cellular assay based on reporter gene for identification of strogenic compounds建立基于荧光素报告基因系统检测雌激素样物质方法的研究
18.An efficient method for screening effective siRNAs using dual-luciferase reporter assay system利用双萤光素酶报告基因系统筛选有效的siRNA
相关短句/例句

Firefly luciferase reporter gene(fluc) vector荧光素酶报告基因载体
3)luciferase reporter vector萤光素酶报告载体pGL3
1.Aim:To construct a luciferase reporter vector with neomycin resistance.方法:设计扩增新霉素抗性基因neo,克隆入萤光素酶报告载体pGL3,得到pGL3-neo。
4)recombined luciferase report vector重组荧光素酶报告基因载体
5)pGL3-Basic vectorpGL3-Basic载体
1.Then P4 promoter gene fragment was inserted into the KpnI-HindⅢsite of pGL3-Basic vector,to construct pGL3-Basic eukaryotic expression vector bearing P4 promoter gene,pGL3-P4,which was further confirmed by double enzyme digestion and DNA sequencing.成功克隆IGF-ⅡP4启动子基因,并构建P4启动子的pGL3-Basic载体。
2.After three promoter segments with different lengths were amplified by PCR amplification,and then the products were identified by DNA sequencing,four promotor segments were inserted into pGL3-Basic vectors.方法运用在线软件Cister对CCDC72基因5’端2000bp进行启动子特征分析;以人毛乳头细胞基因组DNA为模板进行PCR扩增目并进行测序鉴定,再以扩增产物为模板PCR得到不同长度的启动子片段,并测序,将获得启动子片段插入pGL3-Basic载体。
6)luciferase reporter gene荧光素酶报告基因
1.These DNA fragments were inserted into the pGL3-Basic which was the vector of luciferase reporter gene.方法提取人内皮细胞基因组DNA,设计引物克隆EOLA1基因上游不同长度的基因片段,插入到含荧光素酶报告基因的载体pGL3-Basic中,经限制性内切酶酶切鉴定、测序。
2.[Method] Clonging the promoters of CYP3A4 and CYP2B6 which contained the elements that hPXR, a kind of nuclear receptor, can recognize and bind, and inserting the trans-element to the upstream of firefly luciferase reporter gene of the pGL4.方法利用双荧光素酶报告基因系统,将包含hPXR蛋白识别和结合调控元件的CYP3A4和2B6启动子序列插入报告基因上游,将表达载体和报告载体共转染HepG2细胞,用含有利福平或DMSO培养基培养48h后裂解进行双荧光素酶活性检测。
3.Amplified apolipoprotein A-I(apoA-I) promoter gene(376 bp) containing essential transcription regulatory elements from human genomic DNA was fused upstream to the luciferase reporter gene and neomycin resistance gene in the constructed pGL3B-neo vector.PCR扩增含有apoA-I基因的转录调控序列的启动子片断(376 bp),克隆入含有荧光素酶报告基因和新霉素抗性基因的pGL3B-neo载体中,构成受apoA-I启动子调控的重组报告基因质粒pGL3B-neo::apo,稳定转染人肝癌细胞株HepG2。
延伸阅读

PG 又称五倍子酸丙酯、倍酸丙酯。白色至淡褐色结晶性粉末,或为乳白色针状结晶。分子量212.21。无嗅,稍有苦味。水溶液无味。由水或含水乙醇得到一个分子结晶水的盐,在105℃则完全为无水物。易溶于乙醇、丙酮、乙醚。 难溶于氯仿、脂肪、水。溶解度为水中0.35%(20℃),花生油中0.5%(20℃),棉籽油中1.2%(30℃),乙醇中103%(25℃)。水溶液pH值在5.5左右。对热稳定。无水物熔点146-150℃。易与铜、铁离子反应呈紫色或暗绿色。有吸湿性。光线能促进分解。 生产方法:采用酯化法。以正丙醇与没食子酸为原料,浓硫酸为脱水剂,加热进行酯化反应,再将多余的正丙醇蒸馏回收,残留物用活性炭脱包,用蒸馏水或乙醇重结晶而得。 用途:化妆品的主要抗氧剂。在100g化妆品中的最大添加量为0.01g。与BHA和BHT并用有良好的增效作用。与有整合作用的柠檬酸或酒石酸等并用,不仅有增效作用,而且可以防止由金属离子引起的呈色作用。在单独使用时,应避免铁、铜容器。