ISSR-PCR体系,ISSR-PCR system
1)ISSR-PCR systemISSR-PCR体系
1.This paper analyzed the influence of the 6 factors on the reaction results,the factors included:the concentration of Mg2+,dNTP and BSA in ISSR-PCR system,as well as the dosage of the template DNA,primer and Taq DNA polymerase.分析了ISSR-PCR体系中Mg2+、dNTP和BSA浓度,以及模板DNA、引物和TaqDNA聚合酶用量等6个因素对反应结果的影响,建立了适合于台湾水青冈ISSR-PCR扩增的反应体系:10μL PCR反应液中,含10 ng DNA,1。
英文短句/例句

1.Optimization of ISSR-PCR system in Epimedium acuminatum Franch.粗毛淫羊藿ISSR-PCR体系的正交优化
2.Optimization of ISSR-PCR System of Fagus hayatae台湾水青冈ISSR-PCR体系的优化
3.Establishment of Cephalotaxus Oliveri ISSR-PCR Optimal Conditions篦子三尖杉ISSR-PCR体系优化
4.Establishment and Optimization of ISSR-PCR System of Eucalyptus Teretticornis细叶桉ISSR-PCR体系的建立与优化
5.Establishment of ISSR-PCR Reaction System for Swida wilsoniana光皮树无性系ISSR-PCR反应体系的建立
6.Optimization for ISSR Reaction System of jujube tree by Orthogonal Design正交设计优化枣树ISSR-PCR反应体系
7.Optimization of ISSR-PCR Reaction System in Wampee黄皮ISSR-PCR反应体系的优化
8.Optimization for ISSR-PCR Reaction Conditions of Ornamental Peach观赏桃ISSR-PCR反应体系的优化
9.Establishment of ISSR-PCR Reaction System in the Ottelia acuminata(Gagnep.) Dand海菜花ISSR-PCR反应体系的优化
10.Establishment and optimization of ISSR-PCR reaction system of Nelumbo nucifera Gaertn荷花ISSR-PCR反应体系的建立及优化
11.Optimization of ISSR-PCR System of Sweet Potato甘薯ISSR-PCR反应体系的优化
12.Optimization of ISSR-PCR Reaction System in Yane geese雁鹅ISSR-PCR反应体系的优化
13.Optimization of ISSR-PCR Reaction System for Medicinal Plant Gentiana Crassicaulis Duthie Ex Burk粗茎秦艽ISSR-PCR反应体系的优化
14.Optimization of ISSR-PCR Reaction System for Miscanthus floridulus五节芒ISSR-PCR反应体系的优化
15.Optimization for ISSR Reaction System of Monopterus albus with Orthogonal Design黄鳝ISSR-PCR反应体系的建立及条件优化
16.Establishment and Optimization of ISSR-PCR Reaction System for Picea crassifolia kom.青海云杉ISSR-PCR反应体系的建立与优化
17.Establishment and Optimization of ISSR-PCR Reaction System for Earthworm地龙ISSR-PCR反应体系的建立与优化
18.Establishment and Optimization of ISSR-PCR Reaction System of Houttuynia cordata Thunb蕺菜ISSR-PCR反应体系的建立及条件优化
相关短句/例句

ISSR-PCR reaction systemISSR-PCR反应体系
1.In order to establish a stable ISSR-PCR reaction system of Scrophularian ningpoensis Hemsl,some compatible PCR systems were screened by orthogonal design on 5 factors(dNTPs,Taq DNA polymerase,MgCl2,primer and DNA template) and 5 levels,meanwhile the factors and reaction procedure(annealing temperature,elongate time and cyclic numbers) were optimized by single factor gradient.为了建立稳定的玄参ISSR-PCR反应体系,本试验采用5因素(dNTPs、Taq DNA聚合酶、MgCl2、引物及模板)5水平正交设计筛选PCR体系,并利用单因子梯度进一步优化反应体系各因素浓度和扩增程序(退火温度、延伸时间和循环次数)。
2.[Objective] The study aimed to establish the effective and stable ISSR-PCR reaction system for Manglietia grandis Hu et Cheng.[目的]建立高效稳定的大果木莲ISSR-PCR反应体系。
3)ISSRISSR反应体系
1.To optimize ISSR amplification system on Zantedeschia,the effect of four factors, Mg~(2+),Taq DNA Polymerase,primer and DNA,were studied.通过不同浓度Mg~(2+)与TaqDNA聚合酶;模板DNA与引物的组合试验,确定马蹄莲属植物最佳ISSR反应体系为:每20μl中含1×PCR buffer、2。
4)ISSR Rreaction System OptimizationISSR体系优化
5)ISSR reaction systemISSR反应体系
1.The results showed that optimum ISSR reaction system of calla lily was every 20 μL reaction system containing 1×PCR Buffer, 1.选取白花马蹄莲和10个颜色有代表性的彩色马蹄莲栽培品种为材料,采用改进的SDS法提取叶片基因组DNA,通过正交设计研究Mg2+、Taq DNA聚合酶和模板DNA、引物的影响,确定彩色马蹄莲最佳ISSR反应体系为:每20μL中含1×PCR Buffer、1。
2.Results A suitable ISSR reaction system was constructed with the 20 μL reaction system containing 1.所建立的药用菊花ISSR反应体系具有标记位点清晰、反应系统稳定、检测多态性能力较强、重复性好等特点,可用于药用菊花的遗传多样性研究。
6)ISSR-PCR reactionISSR-PCR反应
1.The most suitable ISSR-PCR reaction system and amplified procedure for C.以改良的CTAB法提取的寒兰(Cymbidium kanran Makino)基因组DNA为模板,通过单因子试验建立最适的寒兰的ISSR-PCR反应体系。
延伸阅读

immune PCR分子式:CAS号:性质: 通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上,从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上,进而证明有抗原存在。目前最为敏感的检测方法,理论上可测得一个抗原分子的存在。