发夹RNA,hpRNA英语短句,例句大全

发夹RNA,hpRNA
1)hpRNA发夹RNA
1.The two fragments were ligated in opposite directions into plant expression vector 2300sn,and respectively to produce hpRNAi vector 2300PG.将两个PG5基因片段反向插入到植物表达载体2300 sn的35S启动子和nos终止子之间,构建出可转录表达出发夹RNA结构的组成型油菜RNA干扰载体,为今后油菜利用RNA干扰抗菌核病的基因工程研究奠定了基础。

英文短句/例句

1.Construction of the shRNA expression vectors targeting survivinSurvivin靶向的短发夹RNA表达载体的构建
2.Inhibition of HCV 5'NCR and Core Expression by shRNA and Application of New Gene Carrier HPhA;古菌组蛋白（HphA）介导小发夹RNA抑制HCV 5'NCR和C区表达的研究
3.The Construction of Eukaryotic Expression Vector of shRNA Specific for Survivin Gene and the Effect of Survivin Gene Silencing on HL-60 Cells;Survivin特异性小发夹RNA真核表达载体的构建及其对HL-60细胞的影响
4.Small Hairpin RNA Synthesis and Its Silence Effect on Telomerase Gene Expression in Hela Cells;小片段发夹RNA的制备及其对Hela细胞端粒酶基因沉寂作用研究
5.The Studies on Depressant Effect of Short-Hairpin RNA of Bcl-xL Gene Expression in HepG-2 Cells;短发夹RNA抑制人肝癌HepG-2细胞Bcl-xL基因表达的探讨
6.The Studies on Interference Effect of Short Hairpin RNA on Expression of TGF-β1 Gene in Human Lung Fibroblast;短发夹RNA抑制人肺成纤维细胞TGF-β1基因表达的研究
7.Eukaryotic Vector of the bmi-1 Gene shRNA Construction and Its Effect on LoVo Cellsbmi-1小发夹RNA真核表达载体的构建及对LoVo细胞的影响
8.RNA interfering effects of CCL20 shRNA expressing vectors on human embryonic kidney cellsCCL20基因短发夹RNA表达载体转染人胚胎肾细胞的干扰效应
9.Inhibitory effect of β-catenin short hairpin RNA on the growth of human colon cancer xenografts in nude mice靶向β-catenin短发夹RNA抑制人结肠癌裸鼠移植瘤的实验研究
10.Silence Effect of Short Hairpin RNA on Expression of PIM-1 in Prostate Cancer Cell Lines短发夹RNA对前列腺癌细胞中PIM-1基因沉默效应的研究
11.Construction of LRIG2 Specific Short-hairpin RNA Expressing Vector and Screening of Stably Transfected Cell CloneLRIG2基因短发夹RNA表达载体的构建、鉴定和稳定株的筛选
12.Inhibitory effects of small hairpin RNA on hepatitis B virus X protein in vitro小发夹RNA体外抑制乙型肝炎病毒X蛋白表达的实验研究
13.Inhibitory effect of short hairpin RNA targeting IRAK-M gene for endotoxin tolerance in RAW264.7 cellsIRAK-M短发夹RNA对RAW264.7细胞内毒素耐受性的抑制作用
14.Effect of Suppressing COX-2 Expression by shRNA on Adhesion and Invasion of Human Hepatocellular Carcinoma Cell;短发夹状RNA抑制COX-2表达对人肝癌细胞株黏附侵袭力的影响
15.The Research about Interference of Nucleostemin-Specific Short Hairpin RNA in Vitro and in Vivo;核干细胞因子特异性短发夹状RNA的体内外干扰作用研究
16.Construction and Identification of Multidrug Resistance Gene MDR1 Short Hairpin Expression Plasmids;多药耐药基因MDR1短发夹状RNA表达质粒的构建与鉴定
17.Mechanism of Short Hairpin RNA Silence NF-κB Promotion Apoptosis of Hepatic Stellate Cell;应用短发夹状RNA沉默NF-κB基因促进肝星状细胞凋亡机制研究
18.The Effect of Short Hairpin RNA of Nuclear Factor Kappa B on the Apoptosis of Hepatic Stellate Cell;核因子-κB亚单位p65短发夹状干扰RNA对肝星状细胞凋亡的影响

相关短句/例句

Short hairpin RNA短发夹RNA
1.Construction and identification of a vector expressing short hairpin RNA targeting annexinⅡgene;膜联蛋白Ⅱ基因短发夹RNA表达载体的构建和鉴定
2.The effect of short hairpin RNA on hTERT expression;短发夹RNA对人端粒酶催化亚基表达的影响
3.Objective To clone shRNA (short hairpin RNA) recombinant plasmid targeting on COX-2 gene and analyze the nucleic acid sequence of the recombinant plasmid.目的利用RNA干扰技术,以COX-2为靶基因,构建靶向COX-2基因的短发夹RNA(shRNA)重组表达质粒并进行鉴定分析。
3)shRNA短发夹RNA
1.Inhibition of ICAM-1 gene expression in liver cancer cells by shRNA;短发夹RNA对肝癌细胞细胞间粘附分子-1基因表达的影响
2.Objectives To investigate nano-carrier G9 PAMAM mediated plasmid containing CTGF-shRNA (pCTGF-shRNA) transfection on connective tissue growth factor (CTGF) expression in mouse peritoneal mesothelial cells (MPMCs).目的研究G9PAMAM纳米载体介导的结缔组织生长因子(CTGF)短发夹RNA(shRNA)质粒(pCTGF-shRNA)对小鼠腹膜间皮细胞CTGF表达的影响。
3.The annealed oligonucleotides were inserted into the linearized pSIREN-RetroQ vector to construct the recombinant shRNA plasmid(pSIREN-VEGF-C) and then transformed into E.方法:根据GenBank中VEGF-C序列,设计、合成靶向VEGF-C基因、编码短发夹RNA的两条寡核苷酸序列,退火后用T4连接酶与线性化的pSIREN载体连接,转化感受态大肠杆菌,筛选阳性克隆,抽提质粒,获得重组pSIREN-VEGF-C质粒;BglⅡ和EcoRⅠ双酶切、测序鉴定。
4)small hairpin RNA小发夹RNA
1.Now there are four main kinds of RNA interference libraries with different construction approaches and molecular form,chemically synthesized small interference RNA(siRNA) libraries,chemically synthesized small hairpin RNA(shRNA) libraries,enzymatically prepared siRNA libraries and enzymatically prepared shRNA libraries.不同种类的RNA干扰库已经被用于基因功能的研究之中,根据其构建方式和分子形式的不同,可将RNA干扰库分为4种类型,即化学合成的小干扰RNA(siRNA)库、化学合成的小发夹RNA(shRNA)表达库、酶切法构建的siRNA库和酶切法构建的shRNA表达库。
2.[Methods] A shRNA expressed vector that expresses the specific small hairpin RNA targeting TRAP mRNA was constructed and transfected into SGC-7901 cells, and the stably expressing shRNA cells were selected by G418 and continuously cultured in half the antibiotic concentration for 3 months.目的探讨稳定转染靶向端粒酶调节相关基因TRAP的小发夹RNA对胃癌细胞株SGC-7901的长效影响。
3.[Methods] Plasmid vectors expressing small hairpin RNA (shRNA) targeting at cyclin D1 gene were constructed and transfected into K562 cells mediated by chitosan.[方法]体外构建靶向cyclin D1基因的小发夹RNA(shRNA)表达质粒,通过壳聚糖介导转染K562细胞,Western blot分析检测转染前后cyclin D1蛋白表达变化;集落形成实验检测细胞增殖能力;流式细胞仪检测细胞周期分布及凋亡情况。
5)short hairpin RNA(shRNA)短发夹RNA
1.Objective: To transfect a recombinant short hairpin RNA(shRNA)expression vector targeting Livin gene isoform(BIRC71,BIRC72)into the cervical cancer cell line(Hela cell),in an attempt to observe RNAi-mediated silen- cing on Livin gene and the induction of Hela apoptosis.目的:应用构建成功的Livin异构体(BIRC71,BIRC72)短发夹RNA(short hairpin HNA,shRNA)真核表达载体转染Hela细胞,探讨其对Livin基因的沉默效应及诱导Hela细胞凋亡的作用。
6)shRNA小发夹RNA
1.Construction Expressing Vector of shRNA Inhibiting Cyclin D1 Gene Expression by RNA Interfering;抑制cyclin D1基因小发夹RNA(shRNA)表达质粒的构建
2.[Methods] The 60nt nucleiotide sequence encoded targeting EBNA2 shRNA was cloned into a retroviral vector pSUPER.[方法]用DNA重组技术将60nt能转录产生靶向EBNA2小发夹RNA(smallhairpin RNA,shRNA)的寡核苷酸序列定向插入逆转录病毒载体pSUPER。
3.Objective:To construct and identify the small hairpin RNA(shRNA) expression vector for Smad 4/DPC 4 gene.目的:构建Smad4基因小发夹RNA(shRNA)表达载体。