1)PCR-DGGE聚合酶链式反应-变性梯度凝胶电泳
1.Methods:In 23 Sporadic Alzheimer disease (SAD) patients and 30 cases of normal control,exon3 of CHRNA4 was screened by PCR-DGGE and sequencing.方法用聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)和DNA测序技术分析23例SAD患者及30例正常人的CHRNA4基因外显子3基因序列和16例SAD患者及16例正常人CHRNA7基因全部外显子及其两侧的部分内含子基因序列。
2.The contents of EPS and SMP were determined,and microbial biodiversity was analyzed by PCR-DGGE technology and cloning-sequencing.为了考察活性污泥在营养缺乏的条件下,胞外聚合物(EPS)、溶解性微生物产物(SMP)和微生物种群结构自身的变化情况,为优化MBR系统运行、延缓膜污染等提供理论依据,对天津大学游泳馆MBR中的污泥混合液进行贫营养实验,测定了污泥混合液中EPS和SMP的含量,通过聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)技术和克隆测序技术对微生物多样性进行分析,根据序列数据进行同源性分析并构建系统进化树。
2)polymerase chain reaction-denatur-ing gradient gel electrophoresis(PCR-DGGE)聚合酶链反应-变性梯度胶电泳
3)denaturing/temprature gradient gel electrophoresis变性温度梯度凝胶电泳
4)denaturing gradient gel electrophoresis(DGGE)变性梯度凝胶电泳(DGGE)
英文短句/例句
1.Study of microbial ecosystem diversity by denaturing gradient gel electrophoresis变性梯度凝胶电泳(DGGE)在微生物多样性中的研究
2.DNA Preparation for Analyzing Rhizosphere Soil Microorganism of Strawberry by Denaturing Gradient Gel Electrophoresis(DGGE)适于变性梯度凝胶电泳(DGGE)分析的草莓根际土壤微生物的DNA模板制备
3.Haplotype and Heteroplasmy in the HVⅡ Region of mtDNA in Human Blood Detected by PCR-DGGE;PCR结合变性梯度凝胶电泳法检测人血mtDNA HVⅡ
4.Detection of denaturing gradient gel electrophoresis in pathogenic bacteria of periodontitis牙周炎致病菌的变性梯度凝胶电泳检测
5.Detection of single nucleotide polymorphrism of SHV gene in Acinetobacter baumannii using Denaturing Gradient Gel-Electrophoresis变性梯度凝胶电泳检测鲍曼不动杆菌中SHV基因单核苷酸多态性
6.Evaluation of oral microbial diversity with denaturing gradient gel electrophoresis变性梯度凝胶电泳分析口腔微生物多样性的研究进展
7.Analysis of Fungal Communities Present in Morchella Growth Soil Using Denaturing Gradient Gel Electrophoresis采用变性梯度凝胶电泳研究羊肚菌土壤真菌群落结构
8.Review on Denaturing Gradient Gel Electrophoresis and Its Application in Food Microbiology变性梯度凝胶电泳技术及其在食品微生物研究中的应用
9.Application and Analysis of Denaturing Gradient Gel Electrophoresis in Microbial Molecular Ecology变性梯度凝胶电泳技术在微生物分子生态学研究中的应用
10.Application and Development of Denaturing Gradient Gel Electrophoresis (DGGE) Techniques in Marine Molecular Microbial Ecology变性梯度凝胶电泳技术在海洋微生物分子生态学中的应用
11.Genetic diagnosis for female carriers of glucose-6-phosphate dehydrogenase deficiency by RT-PCR-DGGE逆转录-PCR-变性梯度凝胶电泳法检测葡萄糖-6-磷酸脱氢酶基因缺陷女性携带者
12.Lipoprorein patterns were determined using non-denaturing polyacrylamide gradient gelelectrophoresis to separate LDL particles by size and shape.脂蛋白的形式用非变性聚丙烯梯度凝胶电泳法,根据LDL大小和形状不同来分离LDL粒子。
13.Application of Denaturing Gradient Gel Electrophoresis in the Study of Microalgae Identification and Phytoplankton Genetic Diversity变性梯度凝胶电泳技术在微藻鉴定及浮游植物群落结构研究中的应用
14.Bacterial communities in the phaseⅡ of Agaricus bisporus compost by denaturing gradient gel electrophoresis采用变性梯度凝胶电泳研究双孢蘑菇培养料后发酵过程中的细菌群落结构
15.Denature gradient gel electrophoresis of stratal bacteria activation in oilfield under different pressure conditions不同压力条件下油藏内源细菌群落激活过程中变性梯度凝胶电泳分析
16.STUDIES ON THE ISOLATION NUCLEIC COMPONENT OF BROME MOSAIC VIRUS WITH GRADIENTS GEL ELECTROPHORESIS用梯度凝胶电泳分离雀麦花叶病毒核酸组分
17.pulse alternative field gel electrophoresis脉冲交变电场凝胶电泳
18.denaturing gradient polyacrylamide gel变性梯度聚丙烯酰胺凝胶
相关短句/例句
polymerase chain reaction-denatur-ing gradient gel electrophoresis(PCR-DGGE)聚合酶链反应-变性梯度胶电泳
3)denaturing/temprature gradient gel electrophoresis变性温度梯度凝胶电泳
4)denaturing gradient gel electrophoresis(DGGE)变性梯度凝胶电泳(DGGE)
5)denaturing gradient gel electrophoresis变性梯度凝胶电泳
1.Analysis of mutant frequency of PCR cloning by denaturing gradient gel electrophoresis;用变性梯度凝胶电泳分析PCR克隆的突变率
2.Detection of denaturing gradient gel electrophoresis in pathogenic bacteria of periodontitis牙周炎致病菌的变性梯度凝胶电泳检测
3.Evaluation of oral microbial diversity with denaturing gradient gel electrophoresis变性梯度凝胶电泳分析口腔微生物多样性的研究进展
6)denaturing gradient gel electrophoresis (DGGE)变性梯度凝胶电泳
1.Adopting 16S rDNA of denaturing gradient gel electrophoresis (DGGE) technology to reveal nitrifying bacteria community (ammonium oxidizing bacteria and nitrite oxidizing bacteria) denitrogenation action in constructed rapid infiltrition system (CRI).采用16SrDNA的变性梯度凝胶电泳(DGGE)技术研究人工快速渗滤系统(CRI)中硝化菌群(氨氧化菌和亚硝酸氧化菌)的空间分布规律。
2.After purifying the genomic DNA, 16S rRNA genes were amplified, and the amplified DNA fragments were separated by denaturing gradient gel electrophoresis (DGGE).为了研究生物造粒流化床污水处理反应器颗粒污泥的微生物种群多样性,分别从生物造粒流化床10、60和110cm处取颗粒污泥,通过细胞裂解直接提取颗粒污泥细菌基因组DNA,PCR扩增后经变性梯度凝胶电泳(DGGE)分离,获得微生物群落的DNA特征指纹图谱,对特征条带进行序列测定及序列同源性分析。
延伸阅读
链式反应分子式:CAS号:性质:又称链锁(聚合)反应。在聚合反应过程中,有活性中心(自由基或离子)形成,且可以在很短的时间内,使许多单体聚合在一起,形成分子量很大的大分子的反应。是聚合反应的一大种类。主要包括三个基元反应,即链引发、链增长和链终止。有时还伴随有链转移反应发生。按链活性中心的不同,可细分为自由基型聚合、阳离子型聚合、阴离子型聚合和配位聚合四种类型。