1)Dihydrofolate reductase二氢叶酸还原酶
1.A novel screening method for dihydrofolate reductase inhibitors(DHFRI) using high performance capillary electrophoresis(HPCE) was developed.建立了一种采用毛细管电泳法(CE)测定二氢叶酸还原酶(DHFR)反应动力学参数的新方法。
2.His-tagged Chinese hamster dihydrofolate reductase (DHFR) expression in pET vector system has been reported very low.报道了带有His tag的仓鼠二氢叶酸还原酶基因的克隆和在DB序列增强下T7启动子调控该基因在大肠杆菌中的可溶性高效表达 ,SDS PAGE分析表明 ,带有His tag的仓鼠二氢叶酸还原酶的含量可占大肠杆菌细胞总蛋白质含量的 4 6 % 。
3.49kb DNA fragment of dihydrofolate reductase(DHFR)gene(folA)was ampli-fied from the Escherichia coli DH5αcell lysate.利用PCR技术从大肠杆菌DH5α中获取二氢叶酸还原酶(DHFR)基因folA。
英文短句/例句
1.Prediction of Dihydrofolate Reductase Inhibitors Activity Using Machine Learning Methods;机器学习方法用于二氢叶酸还原酶抑制剂的活性预测
2.Establishment of Methotrexate Enantiomers Resistant A549 Cell Lines of Human NSCCL and Purification of Dihydrofolate Reductase氨甲蝶呤对映体耐药A549细胞株的建立及二氢叶酸还原酶的提取
3.Study of dihydrofolate reductase inhibitors from effective components in chinese herbal medicine by capillary electrophoresis毛细管电泳法研究中草药有效成分对二氢叶酸还原酶的抑制作用
4.The Role of Folate Receptor and Dihydrofolate Reductase in Multidrug Resistance of the Human Breast Cancer Cell Line MCF-7/ADR叶酸受体及二氢叶酸还原酶在人乳腺癌细胞MCF-7/ADR多药耐药中的作用
5.Research of Human Double-Mutant Dihydrofolate Reductase and Cytidine Deaminase Gene Transfected to Murine Bone Marrow Cell;人双突变的二氢叶酸还原酶与胞苷脱氨基酶基因转染小鼠骨髓细胞的实验研究
6.Cloning, Expression, Purification and Protein Cross-linking of Escherichia Coli Dihydrofolate Reductase;大肠杆菌二氢叶酸还原酶的基因克隆、重组蛋白纯化及蛋白质分子间的交联
7.Affinity Capillary Electrophoresis Analyze Dihydrofolate Reductase Activity and Dynamic Parameters of Interaction between Methotrexate Enantiomer and Dihydrofolate Reductase;亲和毛细管电泳法测定二氢叶酸还原酶的活性及与氨甲蝶呤对映体反应动力学参数
8.Association between Methylene Tetrahydrofolate Reductase (MTHFR) Gene C677T Polymorphism and Hyperuricemia;亚甲基四氢叶酸还原酶基因C677T多态性与高尿酸血症的相关性研究
9.Preparation of(R)-1-Phenyl-1,2-ethanediol by Biotransformation with a Coupled System of(R)-Specific Carbonyl Reductase and Formate Dehydrogenase羰基还原酶与甲酸脱氢酶偶联催化制备(R)-苯基乙二醇
10.Study on the Correlation between Hypertensive Disorder Complicating Pregnancy and the MTHFR Gene Polymorphism;亚甲基四氢叶酸还原酶基因多态性与妊娠期高血压疾病的关系
11.Study on Relationship between MTHFR Gene Polymorphism and Ischemic Cerebral Vascular Diseases;四氢叶酸还原酶基因多态性与缺血性脑卒中相关性的研究
12.A Rapid Detection System for C677T Polymorphism of MTHFR Gene;亚甲基四氢叶酸还原酶基因C677T多肽性快速检测系统的建立
13.Gastric Cancer Risk and Methylenetetrahydrofolate Reductase Polymorphisms;亚甲基四氢叶酸还原酶基因多态性与胃癌关系的研究
14.Methylenetetrahydrofolate reductase gene polymorphism and congenital heart disease亚甲基四氢叶酸还原酶基因多态性与先天性心脏病
15.Study on the polymorphysm of MTHFR gene in Hui patient with coronary heart disease in Ningxia宁夏回族冠心病患者亚甲基四氢叶酸还原酶基因多态性的研究
16.Association between Polymorphism of Methylenetetrahydrofolate Reductase C677T and Coronary Heart Disease亚甲基四氢叶酸还原酶基因C677T多态性与冠心病的关联性研究
17.The Relationship between the Polymorphism of MTHFR Gene and Preeclampsia四氢叶酸还原酶基因多态性与子痫前期相关性的研究
18.Polymorphisms of MTHFR among Han population in Henan area河南汉族人群亚甲基四氢叶酸还原酶基因多态性检测
相关短句/例句
DHFR二氢叶酸还原酶
1.Application of dhfr gene negative Chinese hamster ovary cell line to express hepatitis B virus surface antigen;二氢叶酸还原酶基因缺陷细胞株在HBsAg表达中的应用
2.A novel method based on three-dimensional holographic vector of atomic interaction field (3D-HoVAIF) was employed to express 3D structures of 68 dihydrofolate reductase(DHFR) inhibitors and 48 bitter tasting thresholds(BTT).将三维原子场作用全息矢量用于表征68个二氢叶酸还原酶抑制剂和48个苦味二肽结构,分别以多元线性回归和偏最小二乘建模,取得优良结果。
3.A method for fluorophotometric assay of dihydrofolate reductase(DHFR) activity by the use of fluorescence properties of dihydrofolate(DHFA) and NADPH was developed.本文应用二氢叶酸(DHFA)和辅酶Ⅱ(NADPH)的荧光性质,建立了二氢叶酸还原酶(DHFR)活力测定的反应体系,并对酸度等反应条件进行了优化。
3)Dihydrofolate reductase inhibitor二氢叶酸还原酶抑制剂
4)dihydrofolate reductase二氢叶酸还原酶基因
1.METHODS: Double-mutant dihydrofolate reductase-cytidine deaminase gene was transferred into mouse bone marrow cells by retroviral vector.方法:以逆转录病毒为载体,将双突变的二氢叶酸还原酶基因(DH-FR)和胞苷脱氨基酶基因(CD)转染小鼠骨髓细胞,观察该细胞耐MTX及Ara-C的CFU-GM生成情况;RT-PCR检测转基因的表达情况。
2.Objective:To construct complete antibody expression bicistronic vectors via impairing selective marker gene dihydrofolate reductase(dhfr),and to express complete antibody in CHO-dhfr-cells at high level.目的:通过弱化筛选基因二氢叶酸还原酶基因(dhfr),构建双顺反子全抗体表达载体,实现全抗体在CHO细胞中的高效表达。
5)Digestive vacuole transmembrane protein gene(pfcrt)二氢叶酸还原酶基因(pfdhfr)
6)mutated dihydrofolate reductase变异体二氢叶酸还原酶
延伸阅读
二氢叶酸还原酶分子式：CAS号：性质：是催化叶酸还原成四氢叶酸(H4FA)的酶。H4FA形成过程分两步进行，由同一个酶催化，H4FA作为一碳单位载体，为嘌呤核苷酸从头合成和胸嘧啶核苷酸合成等提供一碳单位。氨基蝶呤和氨甲蝶呤的结构类似叶酸，与二氢叶酸还原酶结合的平衡常数为10-10mol/L，比叶酸与该酶的结合能力强1000倍，故是一种极强的竞争性抑制剂。肿瘤细胞经用氨甲蝶呤处理，增殖停止，最终细胞死亡，正是因为核苷酸合成受阻，进而阻断核酸合成的结果。
