1)semi-quantitative RT-PCR半定量RT-PCR
1.Expression of miR393 in Leaves and Panicles at the Booting Stage of Rice under Drought Stress with Semi-Quantitative RT-PCR;半定量RT-PCR方法研究microRNA393在旱处理后的孕穗期水稻叶片和穗部的表达情况
2.Establishment of the semi-quantitative RT-PCR system of nonexpressor of pathogenesis-related genes 1(NPR1) gene in sweet potato(Ipomoea batatas Lam.);甘薯NPR1基因半定量RT-PCR检测方法的建立
3.Aplication of Semi-Quantitative RT-PCR in Analysis Gene Expression Level;半定量RT-PCR法在检测基因表达水平中的应用
英文短句/例句
1.Semi-quantitative RT-PCR and Bioinformatics Analysis of Sheep BTG1 Gene绵羊BTG1基因的半定量RT-PCR及生物信息学分析
2.The Factors Affecting Analysis of Gene Expression Level by Semi-QRT-PCR Technique用半定量RT-PCR法分析基因表达水平的影响因素
3.A Method to Determine Nicotine Biosynthesis with Semi-quantitative RT-PCR利用半定量RT-PCR检测烟碱生物合成的方法
4.A study on the expression of TLR4 in humen colorental cancer by RT-PCR半定量RT-PCR法检测TLR4在人大肠癌中的表达
5.Semi-quantitative RT-PCR and SNPs Techniques to Assess Expression Level and Mutation of TCF3 Gene in Two Chicken Breeds;半定量RT-PCR和SNPs技术分析两个鸡品种TCF3基因的表达水平及多态性
6.Cloning of TLR7 Gene Fragment and Semi-quantitation of mRNA by RT-PCR in Chicken Vaccinated with Infections Bursa Disease Virus;鸡TLR7基因片段的克隆及在鸡IBDV感染下的半定量RT-PCR检测
7.Expression of plasma membrane H~+-ATPase in soybean root by semi RT-PCR systems under low phosphorus stress半定量RT-PCR法检测低磷胁迫下大豆根系质膜H~+-ATPase基因的表达
8.Research on Exogenous Gene mRNA Levels in Zymomonas mobilis by Semi-quantitative RT-PCR半定量RT-PCR检测运动发酵单胞菌中外源基因转录水平的研究
9.Detection of mRNA Gene Expression of BⅢB4 in Fine-wool Sheep Skin by Semi-quantitative RT-PCR Method用半定量RT-PCR法检测BⅢB4基因在绵羊皮肤组织中的mRNA表达
10.Expression Analysis of Bacterial Blight Resistance-Related Gene ME196 in Oryza granulata Baill by Semi-QRT-PCR疣粒野生稻抗白叶枯病相关基因ME196半定量RT-PCR分析
11.Cloning and establishment of the semi-quantitative RT-PCR system of LHR partial gene of Goat's Uterus山羊子宫中LHR基因片段的克隆及半定量RT-PCR检测方法的建立
12.To Detect the Expression of hOCT1 by Semi-quantity RT-PCR in Chronic Myelogeneous Leukemia Patients Administered by Imatinib Mesylate;半定量RT-PCR检测服用甲磺酸伊马替尼慢性粒细胞性白血病患者hOCT1的表达
13.Cloning and Expression Analysis of CHI of Kyoho Grape by Semi-quantity RT-PCR巨峰葡萄查尔酮异构酶(CHI)基因的克隆及表达特性的半定量RT-PCR分析
14.Establishment of the Semi-Quantitative Multiple RT-PCR System of CFL2 GeneCFL2基因半定量多重RT-PCR检测方法的建立
15.Diagnosis Establishment Formulation of RT-PCR、Fluorescence Quantitative PCR and Mutiplex RT-PCR Detection for Japanese Encephalitis Virus;猪乙脑病毒RT-PCR、荧光定量PCR及多重RT-PCR诊断方法建立
16.Quantitative Determination of IFN-γ mRNA by the Competitive RT-PCR Method竞争RT-PCR定量测定IFN-γmrna方法的建立
17.Establishment of Rapid Diagnostic Methods for Detection of West Nile Virus by Nest RT-PCR and TaqMan Real-time RT-PCR;西尼罗病毒巢式RT-PCR和荧光定量RT-PCR两种检测方法的建立
18.Research and Development of Real-time Quantitative RT-PCR for Measurement of Bcr-abl mRNA Transcripts;实时定量RT-PCR检测bcr-abl mRNA的研究
相关短句/例句
Semiquantitative RT-PCR半定量RT-PCR
1.The results analysed by semiquantitative RT-PCR showed that MxFRO was induced by iron-deficiency stress in roots and leaves.半定量RT-PCR结果表明MxFRO在小金海棠的根和叶中均受缺铁胁迫诱导。
2.The Establishment of A Methodology of Semiquantitative RT-PCR with Time-sharing Synchronized Circulation for Detection of E-cadherin Levels in Gastric Cancer Cells;目的:探索建立分时同步循环法分管半定量RT-PCR体系,并检测不同分化程度胃癌细胞中E-钙粘附素的相对表达量。
3.Semiquantitative RT-PCR analysis of apoptotic protease activating factor 1 in oral squamous cell carcinoma;方法:用半定量RT-PCR的方法检测18例正常口腔黏膜组织和32例OSCC组织中APAF1的表达情况。
3)semi-quantity RT-PCR半定量RT-PCR
1.In the present study,a semi-quantity RT-PCR for detection of abundance of liver PEPCK-C mRNA in peripartum dairy cows was established.建立了奶牛肝PEPCK-C mRNA丰度的半定量RT-PCR检测方法,其优化的PCR反应条件(25μL)为:57℃退火、2。
2.Material and Methods By RT-PCR(semi-quantity RT-PCR,SQ-PCR), hOCT1 gene mRNA levels were detected from 9 couples of (18) samples.对象及方法:采用半定量RT-PCR(semi-quantity RT-PCR,SQ-PCR)方法对服用伊马替尼慢性粒细胞白血病患者缓解或耐药前后的2个不同时点的9对(18份)标本进行了hOCT 1基因mRNA水平检测,并对SQ-PCR结果结合临床表现、实验室检查和细胞遗传学结果,分析其水平变化和与疾病缓解或伊马替尼耐药相关性。
3.Expression analysis of CHI of Kyoho grape was carried out by semi-quantity RT-PCR, using grape actin gene as an internal control.试验从一年生巨峰扦插苗中克隆了CHI基因,并采用半定量RT-PCR技术,以actin基因为内参,分析了CHI基因在巨峰葡萄果实不同发育阶段及组织的表达。
4)semi-quantitive RT-PCR半定量RT-PCR
1.The housefly larvae was induced by 30% H2O2 for 90 minutes,and the expression of antibacterial peptide genes of cecropin,defensin,diptericin,attacin,and lysozyme in transcription level was carried out on semi-quantitive RT-PCR.选择30%H2O2诱导家蝇幼虫90 min,应用半定量RT-PCR的方法,以组成型表达的β-actin基因作为内参照,对抗菌活性物质基因cecropin,defensin,dipteri-cin,attacin和溶菌酶基因lysozyme在诱导后的表达情况进行了初步探索。
2.13 differential expression fragments were confirmed by semi-quantitive RT-PCR at different inoculating times.对13个TDFs基因进行了半定量RT-PCR分析,结果表明13个差异片段在对照与处理,或是处理的不同时段存在着表达量的差异。
5)semi-quantitative fluorescent RT-PCR荧光半定量RT-PCR
1.Detection of deltamethrin resistance in Culex pipiens pallen with semi-quantitative fluorescent RT-PCR assay;用荧光半定量RT-PCR检测蚊溴氰菊酯抗性
6)RT-PCR semi-quantity methodRT-PCR半定量法
延伸阅读
半定量分析 准确性比定量分析稍差的分析方法,特点是简单、迅速、费用低。常用于以下几种情况:①希望得知成分的大致含量,以便进一步选择合适的精确定量分析方法;②只要求分析快,不太追求成分的准确含量,例如某种合金型号的确定、工业生产中的中间控制、法医学中对毒物是否超过致死量的鉴定、试剂中杂质是否超过了法定标准的分析;③试样较少,没有理想的定量方法可采用。