VP5基因,VP5 gene英语短句,例句大全

VP5基因,VP5 gene
1)VP5 geneVP5基因
1.According to the published sequence of VP5 gene of IBDV, a pair of specific primers were designed and synthesized.根据GenBank已登录的传染性法氏囊病病毒(IBDV)VP5基因序列,设计合成了一对VP5基因特异性引物,应用RT-PCR技术从IBDV标准毒株中扩增得到VP5基因,并将其克隆到原核表达载体pGEX-6P-1中,构建了重组原核表达质粒pGEX-6P-1-VP5,对重组表达质粒鉴定正确后,转化大肠埃希菌BL21进行诱导表达,SDS-PAGE和Western blot检测结果表明,IBDV VP5基因在大肠埃希菌BL21中得到了正确表达,所表达的融合蛋白与IBDV阳性血清具有特异性抗原抗体反应。
2.Infectious bursal disease virus (IBDV) VP5 gene was amplified and cloned into an N-terminal GST-Tag fusion expression vector, pGEX-4T-2, which was controlled by T7 promoter.利用RT-PCR技术从传染性法氏囊病病毒(IBDV)TL2004株感染鸡胚尿囊液中扩增到VP5基因,进而构建了T7启动子控制下的N端GST-Tag融合表达质粒pGEX-VP5。
3.VP5 gene from MABV genome was amplified by RT-PCR,and was cloned into the 6×His fusion expression vector pET-28a(+),then expressed in Escherichia coli.从海洋双RNA病毒(MABV)基因组中克隆到VP5基因,将其连接到6×His融合表达载体pET-28a(+)上,在大肠杆菌中表达,并对其表达条件进行了优化。

英文短句/例句

1.Cloning and Expression of VP5 Gene from Marine Birnavirus (MABV) in Escherichia coli海洋双RNA病毒(MABV)VP5基因的克隆与表达
2.Construction of Mutant Strains of Infectious Bursal Disease Virus and Functional Research on VP5 Protein鸡传染性法氏囊病病毒基因突变株的构建及VP5蛋白功能研究
3.Construction of Infectious Bursal Disease Virus Chimerics with VP5 Knock-out and Attenuated;传染性法氏囊病病毒VP5缺失减毒株的构建
4.Preparation of monoclonal antibody against VP5 protein of serotype Ⅱ infectious bursal disease virus expressed in E.coliIBDV血清Ⅱ型VP5蛋白原核表达及型特异性单克隆抗体的制备
5.extinguisher loci消失基因座，绝灭基因座
6.Of, relating to, produced by, or being genes or a gene.基因的基因的，与基因有关的，基因产生的
7.Genes are described as DOMINANT or RECESSIVE.基因分为显性基因和隐性基因。
8.hypomorphic allele次等位因素对偶基因
9.Studies on Polymorphism of Prolactin Receptor Gene and Follicle-Stimulating Hormone Beta Subunit Gene in Swine;猪PRLR基因和FSHβ亚基基因多态性研究
10.Research on Hte Microarray Based Gene Mining Algorithm;基于基因表达谱的基因挖掘算法研究
11.Human Genome Project,Post-genome Research and Gene Medicine --New Century of Diagnosis,Pharmaceutics and Therapy with Gene Technique;人类基因组计划、后基因组研究与基因医学——基因诊断、基因制药与基因治疗的新世纪
12.Genetic material produced by gene-splicing.重组基因材料基因分割而产生的基因材料
13.Gene distribution was out of balance. Dominant genes were more than recessive ones.基因的分布是不对称的,显性基因多于隐性基因。
14.Association of apoE Gene, A2M Gene and ACE Gene Polymorphism with Alzheimer s Disease;apoE基因、A2M基因、ACE基因与汉人Alzheimer病的相关性研究
15.Study on Inheritance of the Quality Transgenes Over-expressed in Transgenic Wheat;转基因小麦外源基因品质基因表达的遗传研究
16.Legal Research on Gene Science and Technology--Does Gene Belong to Invention or Discovery;基因科技的法律问题研究——“发明”基因?“发现”基因?
17.Mapping of Genetic Modifiers of Eyal~(bor/bor) Mice定位Eyal~(bor/bor)基因型小鼠Eyal基因的遗传修饰基因
18.Analyses of Genome-wide Disease Resistance Genes and Their Expansion in Medicago truncatula蒺藜苜蓿全基因组抗病基因和基因扩张分析

相关短句/例句

VP2e,VP3 and VP5 geneVP2e、VP3和VP5基因
3)very virulent infectious bursal disease virus VP5 gene鸡传染性法氏囊病超强毒VP5基因
4)VP5 proteinVP5蛋白
1.To study the function of VP5 in vivo and in vitro, two mutant viruses deficient in expressing VP5 protein were generated using attenuated virus (rGt strain) and cell-cultured virulent virus (rGx-F9VP2 strain) as parental viruses by RNA polymerase II system.将带有核酶序列的IBDV基因组A节段插入真核表达载体pCAGGs的β肌动蛋白启动子下游,构建了缺失表达VP5蛋白的IBDV感染性克隆pCGtA△VP5HRT和pCGx-F9VP2A△VP5HRT,将A节段感染性克隆分别与相应B节段感染性克隆pCmGtBHRT或pCmGxBHRT共转染DF I细胞。
5)gene[英][d?i:n][美][d?in]基因
1.Study of the Preparation and the Characteristics of pRc/CMV-BChE Gene-chitosan nanoparticles ①;丁酰胆碱酯酶基因-壳聚糖纳米粒初步研究
2.Correlation between traditional Chinese medicine syndromes in primary immunoglobulin A nephropathy and A267G in 5'-untranslated region within exonal of megsin gene;Megsin基因E1-5’UTR区A267G与免疫球蛋白A型肾病阴虚证的相关性
3.Study on gene chip of leiomyoma of uterus;子宫肌瘤基因芯片的研究
6)Genes基因
1.Study on Apoptosis of Human Leukemia Cells and Its Related Genes Regulation Induced by ~(235) U;浓缩铀诱发细胞凋亡的形态及基因调控
2.Screening of lymphatic metastasis-associated genes in esophageal squamous cell carcinoma;食管癌淋巴结转移相关基因筛选的研究
3.Study on the Genotyping of Aminoglycoside Modifying Enzymes Genes from Pan-drug Resistant Acinetobacter Baumannii.;泛耐药鲍曼不动杆菌氨基糖苷类修饰酶基因研究