1)restriction endounclease sites限制性酶切位点
英文短句/例句
1.The distribution of polymorphism at restriction enzyme site-48 A/ G was analyzed with the method of polymerase chain reaction-restriction fragment length polymorphism( PCR-RFLP).用聚合酶链反应-限制性片段长度多态性方法,分析限制性酶切位点-48A/的多态分布。
2.It enables a specific gene to be located on a particular restriction enzyme fragment.它就能使专一的基因被定位于特定的限制性内切酶切成的片段上。
3.Studies on the Active Groups of Pst I Restriction Endonuclease限制性核酸内切酶PstⅠ活性基团的研究
4.Methods The restriction endonuclease and T4 DNA ligase were used to construct the vector plasmid.方法载体的构建采用限制性内切酶酶切、4DNA连接酶连接等方法。
5.Secondly, special enzymes called extinction enzymes are used to cut the DNA at specific places.第二,利用被叫做限制性内切酶的特殊的酶在特定地方切断DNA。
6.circular restriction map环形限制(酶切)图(谱)
7.Research of Reaction Mechanism and Kinetics Analysis for the FokI Restriction Endonuclease;限制性内切酶FokI作用机制研究及动力学分析
8.Analysis of polymorphism of CYP1A1-MspⅠ gene in patients with breast cancer乳腺癌患者CYP1A1基因MspⅠ酶切位点多态性的分析
9.Application of created restriction site PCR-RFLP to identify alcohol dehydrogenase 2 gene polymorphism应用创造酶切位点PCR-RFLP检测乙醇脱氢酶2基因多态性
10.Large DNA molecules are first dissected with restriction enzymes to produce specific fragments.首先用限制性内切酶将大的DNA分子切断,产生出特殊的片段。
11.Development and Clinical Application of SARS Virus cDNA Restriction Endoenzyme Analysis Kit;SARS病毒cDNA限制性内切酶检测试剂盒的研制和临床应用研究
12.A Novel Fluorescence Assay for the Activity of Restriction Endonuclease Based on Molecular Beacon分子信标检测限制性内切酶活性的新型荧光分析方法
13.Inhibition of Hepatitis B virus expression by malti-target ribozyme in vivo;多位点自剪切核酶以乙型肝炎病毒C基因表达抑制作用的研究
14.Construction of Protoplast-Based Transformation System of Fungal Biocontrol Agent Paecilomyces Fumosoroseus with Restriction Enzyme-Mediated Integration (REMI);限制性内切酶介导的玫烟色拟青霉原生质转化体系构建
15.The Restriction Endonuclease Analysis and Elimination of pR_(st98) in Salmonella typhi;伤寒杆菌质粒pR_(st98)的DNA限制性内切酶分析和药物的体外消除研究
16.Inserter Mutation by Restriction Enzyme-mediated Integration and Agrobacterium tumefaciens-mediated in Trichoderma koningii and Isolation of the Mutant限制性内切酶和农杆菌介导的康宁木霉插入突变及突变体的筛选
17.Choice of restriction endonuclease and establishment of silver-staining method in cDNA-AFLP System for Carthamus tinctorius红花cDNA-AFLP限制性内切酶的选择及银染体系的建立
18.Identification of Thermus to the Species Level using 16S rDNA Gene Restriction Fragment Patterns利用针对16S rDNA序列的限制性酶切分析鉴定栖热菌属
相关短句/例句
restriction site限制酶切位点
3)amplication-created restriction site扩增引进限制性酶切位点
4)CRS-PCR创造限制酶切位点PCR法
1.Created restriction site PCR(CRS-PCR) uses mismatches in one of the two PCR primers flanking the single nucleotide polymorphism to create or remove a restriction endonuclease recognition site in order to identify SNP genotypes.创造限制酶切位点PCR法是应用引物错配技术结合单核苷酸多态性(SNP)而配合成一个酶切位点,使SNP可用于PCR-RFLP分析的一种方法。
5)restriction enzyme digestion限制性酶切
1.To establish the technique of RT nested PCR for diagnosis of hemorrhagic fever with renal syndrome(HFRS), and typing of the amplified product was carried out by restriction enzyme digestion.为建立检测肾综合征出血热 (HFRS)患者血清中汉坦病毒 (HV)基因的RT nestedPCR方法 ,并进一步进行限制性酶切分型 ,设计并合成互补于HVS基因片段的引物 ,对 78例HFRS患者血清进行RT nestedPCR检测 ,并对PCR产物进行酶切分析。
6)restriction digestion限制性酶切
延伸阅读
限制分子式:CAS号:性质:一种保护细菌细胞不受外源DNA侵入的作用,通过限制性内切酶实现。该酶对双链DNA进行定点剪切。通常合成限制性内切酶的细胞由于还能合成识别同样位点的修饰酶而使细胞本身的DNA结构进行修饰,如甲基化而受到保护。当细菌受到噬菌体感染,而噬菌体DNA事先未经修饰,则这未经修饰的外来噬菌体DNA被切成小片段,再进一步为外切核酸酶所降解。未经修饰的噬菌体DNA引起感染的可能性比修饰过的噬菌体DNA要小几个数量级。