轻链可变区,VL
1)VL轻链可变区
1.The　VL　gene　fragment　of　antihuman　C1INH　McAb　was　amplified　by　RTPCR　using　a　pair　of　oligonucleotide　primers　which　were　designed　according　to　FR1　and　FR4　gene　sequences　of　mouse　immunoglobulin　variable　region　of　light　chain　(VL).获得抗体的可变区基因是由鼠源性ＭｃＡｂ制备重组抗体的前提，本实验合成与轻链可变区（ＶＬ）ＦＲ１和ＦＲ４互补的通用引物，由分泌抗人Ｃ１－ＩＮＨＭｃＡｂ的杂交瘤Ｆ７细胞株提取总ＲＮＡ，经ＲＴ－ＰＣＲ扩增出Ｆ７ＶＬ的ｃＤＮＡ片段，并将其克隆入ｐＵＣ１８／１９测序载体，从两端进行双脱氧核苷酸随机终止法的ＤＮＡ序列测定。
英文短句/例句

1.Ig VL cDNA sequence and diversity analysis of Hucho taimen (Pallas)哲罗鱼免疫球蛋白轻链可变区序列及其多样性
2.Amplification, Cloning and Sequence Analysis of the Genes of V_H and V_K of an Anti-human CD3 Monoclonal Antibody;抗人CD3单抗重、轻链可变区基因的体外扩增、克隆和序列分析
3.Cloning and Nucleotide Sequencing of Light Chain Variable Genes from Species-specific Monoclonal Antibodies against E.acervulina鸡堆型艾美耳球虫特异性单抗轻链可变区基因的克隆与序列测定
4.THE PREPARATION OF GENE LIBRARIES OF LIGHT AND HEAVY CHAIN VARIABLE REGIONS OF MOUSE S ANTIBODIES TO HUMAN TRANSFERRIN;鼠抗人转铁蛋白抗体轻链与重链可变区基因库的制备
5.Effect of domain order on the activity of single chain antibody derived from the monoclonal antibody against H antigen on human erythrocyte抗红细胞H抗原单链抗体中可变区重链和轻链顺序对其活性的影响
6.Amplification, Cloning and Expression of Variable Gene from Anti-human Hepatocarcinoma Monoclonal Antibody and Identification of the Expressed Recombinant Phage Antibody.;抗人肝癌单抗轻、重链可变区基因的体外扩增、克隆、表达及初步鉴定
7.Amplified and sequence analysis of V_H and V_L genes of monoclonal antibody against Cysticercus cellulosae抗猪囊尾蚴单克隆抗体轻重链可变区基因的扩增及序列分析
8.Cloning and nucleotide sequencing of heavy and light chain variable genes from species-specific monoclonal antibodies against E.acervulina鸡堆型艾美耳球虫特异性单抗轻、重链可变区基因的克隆与序列测定
9.Screening and identification of antibody single chain variable fragment against HIV gp120HIV-gp120人源单链可变区抗体筛选与鉴定
10.pageable link pack area可分页的链接装配区
11.Single Chain Fv Antibody for HIV-1 Gene Therapy and Its Optimized Production;单链可变区抗体在HIV-1感染基因治疗中的应用及其标准化制备
12.Construction, Expression and Identification of Anti-AFP V_H Single-domain Antibody Fusion Protein Gene;抗AFP重链可变区单域抗体融合蛋白的构建、表达及初步鉴定
13.The Construction of Variable Regions of scFv Library Against the Merozoite of Eimeria Acervulian;鸡堆型艾美耳球虫裂殖子单链抗体可变区基因文库的构建
14.Analysis of the Immunoglobulin Heavy Chain Variable Region Gene Fingerprinting of Human Neonates;新生儿免疫球蛋白重链可变区基因指纹图谱分析
15.Spatial-Temporal Dynamics of Fujian Regional Convergence with Spatial Markov Chains Approach;福建省区域趋同时空演变的空间马尔可夫链分析
16.Preparation of Monoclonal Antibodies Against Subtype H1 of Swine Influenza Virus and Cloning Variable Region of Heavy Chain Gene抗H1亚型猪流感病毒单克隆抗体的制备及重链可变区基因的克隆
17.Development of a high-efficient assay for amplifying Vβ-encoding genes of human T-cell receptor一种高效扩增人T细胞受体β链可变区基因的方法建立
18.A high-performance method for amplifying human T-cell receptor Vα-encoding genes一种高效扩增人T细胞受体α链可变区基因方法的建立
相关短句/例句

VL gene轻链可变区基因
1.We amplified VL gene of mouse McAb anti-stomach cancer by PCR from genomic DNA of3G, hybridoma cells and clolled it into pBluescript vectors.本文报导了用ＰＣＲ方法（ＰｏｌｙｍｅｒａｓｅＣｈａｉｎＲｅａｃｔｉｏｎ）从抗胃癌单抗３Ｇ９杂交瘤细胞的基因组ＤＮＡ中，扩增出３３３ｂｐ的轻链可变区基因，克隆至ｐＢｌｕｅｓｃｒｉｐｔⅡｋｓ十／一载体上，筛选到阳性克隆。
3)variable region of heavy chain (VH)抗体轻链可变区
4)variable region gene of heavy chain andlight chain可变区重链和轻链基因
5)Vk gene clone轻链可变区基因克隆
6)VH and VL genes轻重链可变区基因
1.The VH and VL genes were amplified from the hybridoma cell secreting monoclonal antibody against Cysticercus cellulosae by RT-PCR.从分泌抗猪囊尾蚴单克隆抗体杂交瘤细胞提取总RNA,以此为摸板,RT-PCR扩增出抗猪囊尾蚴单克隆抗体轻重链可变区基因,琼脂糖凝胶电泳检测显示约350 bp,符合鼠抗体特征。
延伸阅读

链可变菌素分子式：CAS号：性质：又名曲张链菌素；链可变菌素。其复合物从乙酸乙酯/己烷中重结晶后，得黄色结晶。用稀碱处理后，水解为中性或弱酸性物质。在室温pH值2.0～6.0条件下稳定3～4天，在pH值7.8时2～4天后逐渐失去抗菌活性。链霉菌Streptomyces spectabilis产生的安莎类抗生素。分离出A～G与J八个组分，均为黄橙色结晶或粉末。熔点依次为200～201℃，187～189℃，189～199℃，172～175℃，198～202℃，222～224℃，190～192℃，与177～180℃；比旋度-31(氯仿)各为+160°(c=0.118)，+576°(c=0.191)，+602°(c=0.14)，+590.3°(c=0.155)，+412.3°(c=0.123)，-，+473°(c=0.074)与+436°(c=0.094)。复合物具有抗革兰氏阳性菌包括结核杆菌活性，并有抑制痘苗病毒和小鼠Raucher白血病病毒依赖RNA的DNA聚合酶作用。