丁肝病毒核酶,HdvRz英语短句,例句大全

丁肝病毒核酶,HdvRz
1)HdvRz丁肝病毒核酶
1.According to the gene sequences that had published in GenBank of Newcastle disease virus NA-1 from goose(GPMV),a pair of specific primers were designed for amplifying the two fragments of leader and trailer that were ligased with T7 promoter,HdvRz and T7 terminator with overlap PCR,then cloned them to the pVAX-1 which had been reconstituted.根据GenBank上已发表的NA-1株GPMV全序列设计并合成2对特异性引物,克隆得到GPMV的Leader及Tralier序列,与T7启动子、丁肝病毒核酶序列及T7终止子重叠PCR连接后,将连接产物克隆至改造的pVAX-1载体中,并用增强型绿色荧光蛋白基因代替GPMV的整个编码区,只保留与病毒复制、转录和病毒包装相关的调控序列,酶切、测序及荧光鉴定后,与pCI-NP、pCI-P及pCI-L等3个辅助质粒共转染可表达T7RNA聚合酶的VT7细胞系,结果绿色荧光蛋白得到表达,表明成功构建了"拯救"病毒的微型基因组。
2)Hepatitis delta virus ribozyme丁型肝炎病毒核酶
3)virus ribozymes病毒核酶
1.In vitro self-cleavage activity of hepatitis delta virus ribozymes with different length and its significance;不同长度丁型肝炎病毒核酶的体外自剪切活性及其意义
4)HDV丁肝病毒(HDV)
5)hepatitis delta virus丁型肝炎病毒
1.Aim: To obtain recombinant hepatitis delta virus antigen and investigate its application to diagnosis to serum antibody to HDV.目的 :获得能够应用于丁型肝炎病毒 (HDV)酶免试剂的重组丁型肝炎病毒抗原。
2.in this rescarch,with PSVLD3 as template,a 139bp cDNA containing the region related tohepatitis delta virus (HDV)genomic RNA ribozyme,which is able to undergo autocatalytic cleavage,wasamplified by PCR.以质粒（ｓＳＶＬＤ３）为模板，通过聚合酶链反应（ＰＣＲ）扩增得到一条１３９ｂｐ的片段，它含有丁型肝炎病毒（ＨＤＶ）基因组ＲＮＡ中核酶（ｒｉｂｏｚｙｍｅ）区的ｃＤＮＡ，该核酶具有自身裂解功能，将上述片段插入到ｐＧＥＭ－３Ｚ中，经筛选、鉴定，得到一重组质粒（ｐＨＤＶ１０８），经测序发现有２个碱基变异，以该质粒为模板，通过Ｔ７ＲＮＡ聚合酶，转录出核酶的前体，并观察到其自身裂解产物，自裂率达７１％。

英文短句/例句

1.Study on the Inhibition of Hepatitis B Virus (HBV) by Hepatitis Delta Virus (HDV) Ribozyme in Cells;丁型肝炎病毒（HDV）核酶对乙型肝炎病毒（HBV）抑制作用的研究
2.Expression of the hepatitis Delta antigen in prokaryotic cell and evaluation of its application as an EIA diagnostic reagent丁型肝炎病毒抗原的原核表达及抗原性分析
3.Seroepidemiological Survey of Hepatitis D Virus Infection in New Military Recruits北京军区新兵人群丁型肝炎病毒感染血清流行病学调查
4.HIGH- LEVEL EXPRESSION AND SECRETION OF CHINESE HENAN HEPATITIS DELTA ANTIGEN IN PROKARYOTIC CELLS我国河南株丁型肝炎病毒抗原在原核细胞中的高效表达及分泌
5.non A non B hepatitis virus非甲非乙型肝炎病毒
6.The influence of viral characteristics on the pathogenesis of severe hepatitis B乙型肝炎病毒特性对重型肝炎发病机制的影响
7.Relationship between the Genotype of Hepatitis B Virus and Hepatitis B Virus Related Hepatocellular Carcinoma;乙型肝炎病毒基因型与乙型肝炎病毒相关的肝癌的关系
8.The infection rate of hepatitis C virus in Chinese patients with type 2 diabetes mellitus2型糖尿病患者丙型肝炎病毒感染率
9.Study on pathogenicity of subtype 4a swine HEV in rhesus monkey4a亚型猪戊型肝炎病毒对猕猴致病性
10.Hepatitis B virus X gene mutation and hepatocellular carcinoma乙型肝炎病毒X基因突变与肝细胞癌
11.Advancements in hepatitis B virus associated glomerulonephritis乙型肝炎病毒相关性肾炎的研究进展
12.Evidence-based treatment for a patient with hepatitis B virus associated glomerulonephritis presenting as nephrotic syndrome乙型肝炎病毒相关性肾炎的循证治疗
13.Hepatitis B Virus Genotyping and the Influence on Chronic Hepatitis B乙型肝炎病毒基因分型及对肝病的影响
14.Infection of Hepatitis E Virus in Dali大理地区戊型肝炎病毒感染病例分析
15.Dynamic Study on Pathology of Experimental New Duck Viral Hepatitis;新型鸭病毒性肝炎病理学的动态研究
16.Impact of Hepatitis B and Hepatitis C virus Infections on Patients and Allograft Outcomes in Renal Transplant Recipients乙型肝炎病毒和丙型肝炎病毒感染对肾移植受者长期预后影响
17.Study on the Traditional Chinese Medicine Types of Syndrome of Chronic Hepatitis B;慢性病毒性乙型肝炎中医证型的研究
18.Establishment of hepatitis B virus-transfected HepG2 cell model乙型肝炎病毒感染HepG2细胞模型的构建

相关短句/例句

Hepatitis delta virus ribozyme丁型肝炎病毒核酶
3)virus ribozymes病毒核酶
1.In vitro self-cleavage activity of hepatitis delta virus ribozymes with different length and its significance;不同长度丁型肝炎病毒核酶的体外自剪切活性及其意义
4)HDV丁肝病毒(HDV)
5)hepatitis delta virus丁型肝炎病毒
1.Aim: To obtain recombinant hepatitis delta virus antigen and investigate its application to diagnosis to serum antibody to HDV.目的 :获得能够应用于丁型肝炎病毒 (HDV)酶免试剂的重组丁型肝炎病毒抗原。
2.in this rescarch,with PSVLD3 as template,a 139bp cDNA containing the region related tohepatitis delta virus (HDV)genomic RNA ribozyme,which is able to undergo autocatalytic cleavage,wasamplified by PCR.以质粒（ｓＳＶＬＤ３）为模板，通过聚合酶链反应（ＰＣＲ）扩增得到一条１３９ｂｐ的片段，它含有丁型肝炎病毒（ＨＤＶ）基因组ＲＮＡ中核酶（ｒｉｂｏｚｙｍｅ）区的ｃＤＮＡ，该核酶具有自身裂解功能，将上述片段插入到ｐＧＥＭ－３Ｚ中，经筛选、鉴定，得到一重组质粒（ｐＨＤＶ１０８），经测序发现有２个碱基变异，以该质粒为模板，通过Ｔ７ＲＮＡ聚合酶，转录出核酶的前体，并观察到其自身裂解产物，自裂率达７１％。
6)Hepatitis D virus丁型肝炎病毒
1.Methods The serum hepatitis D virus markers(HDVM:HDAg and anti-HD)and hepatitis B virus markers(HBVM:HBsAg,anti-HBs,HBeAg,anti-HBe and anti-HBc)were detected with enzyme linked immunosorbent assay(ELISA)and the parameters of liver function were measured with OLYMPUS AU 400 automatic biochemistry analyzer in 312 HBsAg carriers.目的探讨乙型肝炎病毒(HBV)与丁型肝炎病毒(HDV)重叠感染后血清病毒性肝炎标志物和肝功能变化。