1)Tet-On expression system四环素基因表达调控系统
1.Methods A single plasmid vector containing the regulatory element and TAg expression element of Tet-On expression system driven by rat neuron-specific enolase(NSE) promoter was constructed and micro-injected into the male pronuclei of fertilized ova from F1 hybrid mice(C57BL/6J×CBA).方法构建由大鼠神经元特异性烯醇化酶基因(rat neuron-specific enolase,NSE)启动子驱动四环素基因表达调控系统的载体,从而控制SV40 TAg基因的表达;受精卵雄原核显微注射该线性化载体制备转基因小鼠;PCR方法鉴定首建鼠;RT-PCR方法检测小鼠组织中四环素反式激活子(rtTA)及SV40 TAg基因的表达情况。
英文短句/例句
1.Expression of β-galactosidase gene mediated by tetracycline-regulated gene expression system in HepG2 cells四环素基因表达调控系统调控β-半乳糖苷酶基因在肝癌细胞中的表达
2.Preparation and Expression Analysis of TRE-C-imp13 Transgenic Mice That Based on Tet System基于四环素调控系统的TRE-C-imp13转基因小鼠的构建及表达分析
3.Studies on the Plant Gene Expression System Controlled by Heat Shock and Tetracycline;热激与四环素双重诱导调控的植物基因表达系统研究
4.Study of Liver-specific Double Transgenic Mice of Tight Tet-on Expression System严密型四环素调控系统的肝脏特异表达的双转基因小鼠模型的研究
5.The Study of HSV-tk Gene Expression and Antitumor Activity Mediated by Tetracycline-regulatable System in Vitro四环素调控HSV-tk基因表达及体外杀伤肿瘤细胞的研究
6.Tetracycline-regulated Systems Based on the Viral Vector for Gene Therapy Applications基于四环素调控系统的病毒载体在基因治疗中的应用
7.A Study on Transformation of a Genetic Engineering Male Sterile System Conditioned by Tetracycline in Rice;受四环素调控的基因工程雄性不育系统导入水稻的研究
8.Construction and Identification Inducible Transgenic Expression System of Plasmodium Falciparum Based on Tetracycline;恶性疟原虫四环素诱导性转基因表达系统的构建与检测
9.Basic studies of tetracycline-controlled system on expression of DT/VEGF chimeric gene on gastric cancer therapy四环素调控系统控制白喉毒素-血管内皮生长因子融合基因治疗胃癌的基因研究
10.Expression of Interleukin-2 Gene Regulated by RU486 SystemRU486系统调控的IL-2基因的表达
11.Comparative Transcriptome Analysis of Yersinia Pestis in Response to Various Environmental Stresses;环境因素调控鼠疫耶尔森氏菌基因表达的比较转录谱学研究
12.Human β-defensin-1 Gene Expression and Transcription Regulation人β-防御素-1基因表达与转录调控
13.Establishment of Tetracycline-controlled A30P Mutation α-synuclein Transgenic Mice四环素调控A30P突变α-synuclein转基因小鼠的构建
14.Regulating A20 Gene Expression in Hepatic Metastic Subline of Nasopharyngeal Carcinoma by Tet-on SystemTet-on系统调控A20基因在鼻咽癌肝转移亚系中的表达
15.Cell Adhesion, Influence Factors and Adhesion Molecule Gene Modulation细胞粘附、影响因素及粘附分子基因表达的调控
16.Preliminary Study on Regulable DNA Vaccines Against Plasmodium Falciparum;应用四环素调控系统建立可调控抗恶性疟原虫DNA疫苗的初步研究
17.Studies on Expression and Regulated Mechanism of Four Tumor Suppressor Candidate Genes in NPC;四个候选抑瘤基因在鼻咽癌中的表达及表达调控机制的研究
18.MEF2C regulats DOK5 gene expressionMEF2C调控DOK5基因的表达
相关短句/例句
Tetracycline controlled gene expression system四环素调控基因表达系统
3)Tet-On regulatory system四环素调控基因表达
4)Tetracycline-regulated gene expression system四环素调控表达系统
5)Gene expression regulation system基因表达调控系统
6)Tc-controlled expression SystemTet调控的基因表达系统
延伸阅读
基因表达系列分析基因表达系列分析（sage）是通过快速和详细分析成千上万个est（express sequenced tags）来寻找出表达丰富度不同的sage标签序列。再次访法中，通过限制性酶切可以产生非常短的cdna（10-14bp）标签，并通过pcr扩增和连接，随后对连接题进行测序。sage大大简化和加快了3’端表达序列标签的收集和测序。同dd一样，sage是一个“开放”的系统，可以发现新的未知的序列。在进行标本的比较之前，sage在cdna的产生和处理上需要较多个步骤。由于sage是一个依赖dna测序的基因计量方法，它对基因表达的测定比dd更加量化。由于需要进行大量的测序反应，所以费用因素使大多数研究机构对其广泛应用的主要限制。
