1)RT-PCR逆转录聚合酶链式反应
1.Detection of nervous necrosis virus from five cultured groupers (Epinephelus spp.) using RT-PCR;逆转录聚合酶链式反应(RT-PCR)检测5种养殖石斑鱼的神经坏死病毒
2.Isolate and clone SYT-SSX cDNA into PUCm-T vector using RT-PCR and then sequence it on American automatic capillary electrophoresis genetic analyzer.利用逆转录聚合酶链式反应(RT-PCR)方法获得SYT-SSX的cDNA。
3.Methods:Reverse transcriptase polymerase chain reaction(RT-PCR) was performed on endometriosis tissues obtained from controls (n=20) and on eutopic/ectopic endometriosis tissues from endometriosis patients (n=30) to evaluate eNOS and iNOS concentrations in these endometriosis tissues.方法:采用逆转录聚合酶链式反应(RT-PCR)方法对20例正常子宫内膜和30例子宫内膜异位症患者在位和异位内膜进行内皮型NOS(eNOS)和诱导型NOS(iNOS)表达的半定量检测。
英文短句/例句
1.Detection of Rice Stripe Virus by Reverse Transcription and Polymerase Chain Reaction逆转录聚合酶链式反应快速检测水稻条叶枯病毒
2.DETECTION OF ENTEROVIRUS RNA IN HUMAN BLOOD SPECIMENS BY REVERSETRANSCRIPION POLYMERASE CHAIN REACTION逆转录聚合酶链式反应检测人血标本中肠道病毒核糖核酸
3.Dection of enterovirus infection by reverse transcription and polymerase chain reaction应用逆转录聚合酶链反应检测肠道病毒感染
4.Detection of mRNA in kidney of dead rat using fluorescence semi-quantitative RT-PCR;荧光半定量逆转录-聚合酶链式反应检测大鼠死后肾mRNA
5.Establishment of reverse transcription semi-nested polymerase chain reaction to the pathogenic diagnosis of JE patients逆转录-聚合酶链反应在乙型脑炎诊断中的建立
6.Multiplex Reverse Transcription-nPCR: Application in the Study of Causative Organisms of Viral Encephalitis in Children;多重逆转录套式聚合酶链式反应在儿童病毒性脑炎病原学研究中的应用
7.Rapid identification of subtypes of respiratory syncytial virus by real-time reverse transcription PCR实时逆转录聚合酶链反应快速鉴别呼吸道合胞病毒亚型
8.Research of Real-time Quantitative Reverse-transcriptase Polymerase Chain Reaction (RQ-PCR) to Detect bcr/abl Fusion Gene in Chronic Myeloid Leukemia;实时定量逆转录聚合酶链反应检测慢粒bcr/abl融合基因的研究
9.Expression of the multidrug resistance in human bladder tumors by reverse transcription polymerase chain reaction应用逆转录聚合酶链反应技术检测膀胱癌多项耐药基因的表达
10.The detection of circulating hepatocellular carcinoma cells in peripheral venous blood by reverse transcription polymerase chain reaction and its clinical significance巢式逆转录多聚酶链反应检测循环性肝癌细胞及其临床意义
11.Reverse transcription multiplex-nPCR:application in the study on causative organisms of viral encephalitis in children逆转录多重套式聚合酶链反应在儿童病毒性脑炎病原学研究中的应用
12.DETECTION OF FISH VIRAL HEMORRHAGIC SEPTICEMIA VIRUS (VHSV) BY NESTED REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION (RT-PCR)应用巢式逆转录聚合酶链反应检测鱼类病毒性出血性败血症病毒(VHSV)
13.Analysis of reserved conditions of blood sample for detection of HCV RNA with RT-PCR逆转录聚合酶链反应检测HCV RNA血标本保存条件分析
14.Comparison Study of Conventional Method and Reverse Transcription Polymerase Chain Reaction on Detection of Flavivirus in Mosquitoes逆转录聚合酶链反应与常规法检测蚊体内黄病毒的比较研究
15.Development of a SYBR Green Real-time RT-PCR for Detection of Mumps Virus GeneSYBR Green荧光逆转录-聚合酶链反应检测流行性腮腺炎病毒基因
16.Studies on the Detection of Bovine Viral Diarrhea-mucosal Disease Virus Using Reverse Transcription-nested Polymerase Chain Reaction;反转录—套式聚合酶链式反应检测牛病毒性腹泻/粘膜病病毒的研究
17.Studies on the Detection and Differentiation of Virulent and Avirulent Strains of Newcastle Disease Virus in Chickens Using Reverse Transcription-Nested Polymerase Chain Reaction;反转录—套式聚合酶链反应检测、鉴别鸡新城疫病毒强、弱毒株的研究
18.Rapid Detection of Tobacco Mosaic Virus from Crude Samples on Oscillatory-flow Reverse Transcription Polymerase Chain Reaction Microfluidics微流控振荡流反转录聚合酶链式反应快速检测烟草花叶病毒
相关短句/例句
Reverse transcriptase polymerase chain reaction逆转录聚合酶链式反应
1.Immunohistochemistry and reverse transcriptase polymerase chain reaction(RT-PCR) were used to identify the expression of erythropoietin, respectively.采用线栓法制作大鼠局灶性缺血再灌注模型 ,以免疫组织化学和逆转录聚合酶链式反应(RT PCR)技术 ,检测缺血再灌注不同时间脑内Epo的表达变化。
2.Methods:The degree of drug resistance was detected by MTT method ;the expression of TopoⅡ gene in cell lines BIU 87/ADM and BIU 87 was detected with reverse transcriptase polymerase chain reaction.方法 :应用MTT法检测人膀胱癌耐药细胞系 (BIU 87 ADM)的耐药性 ;应用逆转录聚合酶链式反应 (RT PCR)分别对BIU 87 ADM及敏感细胞系 (BIU 87)中TopoⅡ基因进行检测。
3.Methods The expression and the products of Topo Ⅱ gene in cell line BIU 87/ADM and BIU 87 were detected with reverse transcriptase polymerase chain reaction and immunohistochemistry.方法 应用逆转录聚合酶链式反应 (RT PCR)和免疫细胞化学技术 ,分别对人膀胱癌耐药细胞株 (BIU 87 ADM)及敏感细胞株 (BIU 87)中TopoⅡ基因及其产物的表达进行研究。
3)Reverse transcription polymerase chain reaction逆转录聚合酶链式反应
1.The effect of cerium nitrate on expression of CaMⅠ, PMCA1b and mRNA in rat hepatocyte was studied by means of reverse transcription polymerase chain reaction.运用逆转录聚合酶链式反应研究了硝酸铈对大白鼠肝脏CaMⅠ ,PMCA1b基因表达的影响。
2.Reverse transcription polymerase chain reaction(RT-PCR) was used to detect Alpha-fetoprotein mRNA(AFP mRNA) in peripheral blood of 78 patients with primary hepatocellular carcinoma(HCC).采用逆转录聚合酶链式反应(RT-PCR)检测78例原发性肝细胞癌(HCC)患者及109例其它疾病组患者外周血AFP mRNA的表达,同时用微粒子酶免疫法检测血清AFP水平。
4)reverse transcription-polymerase chain reaction逆转录聚合酶链式反应
1.Expression of the mRNA for intrarenal transforming growth factor-β 1 (TGF-β 1) and renin was measured by reverse transcription-polymerase chain reaction (RT-PCR).方法 :大鼠灌服CsA及低盐饮食 2 8天 ,用逆转录聚合酶链式反应 (RT PCR)的方法观察肾内转化生长因子 β1(TGF β1)、肾素的mRNA表达及TGF β1、Ⅳ型胶原 (CollagenⅣ )免疫组化的改变 ,并观察复方丹参注射液对上述改变的影响。
5)RT PCR逆转录聚合酶链式反应
6)RT-PCR逆转录-聚合酶链式反应
1.Detection of Several RNA Viruses in Fruit Trees by RT-PCR;逆转录-聚合酶链式反应检测果树RNA病毒
2.A First Attempt to Apply RT-PCR Technique to Laboratory Diagnosis of Measles;逆转录-聚合酶链式反应用于麻疹实验室诊断初探
3.METHODS The leukomonocyte was separated from renal transplant recipient peripheral blood,RNA was isolated before RT-PCR reaction.目的利用半定量逆转录-聚合酶链式反应(RT-PCR)技术,测定细胞因子白细胞介素-2(IL-2)、白细胞介素-4(IL-4)、干扰素-γ(IFN-γ)mRNA表达水平,研究其在肾移植患者术后,钙调磷酸酶抑制剂治疗的疗效评价中的应用。
延伸阅读
聚合酶链式反应分子式:CAS号:性质:又称基因体外扩增技术或核酸体外扩增技术。利用两种与相反链杂交并利用附着于目标DNA两端的寡核苷酸引物在高温(95℃)使含目标DNA片段的DNA双链变性,分解为单链。在较低温度(37~63℃)与引物退火:然后变温到72℃左右,在耐高温DNA聚合物酶作用下引物被沿样板DNA单链延伸合成互补链,然后又变性→退火→延伸反复进行。引物大大过量,dNTP过量,酶在高温中是较稳定的,这样经过几十个循环,目标DNA片段就按2n数递增。用此法可从mRNA中,cDNA库中扩增出目标基因。过去一般合成500~1000bp较好,现已发展出大片段(75kb)的PCR,且差错甚少。PCR技术的发展还可用于定点突变,质粒重组及FLP等方面。这一技术的作用范围日益扩大,已成为分子生物学工作者基本技术之一。这项专利技术1985年由美国塞特斯(Cetus)公司开发。是20世纪80年代分子生物学领域中的一项革命性突破,已在分子生物学、医学、法学等领域发挥了极大的作用。