1)quantitative polymerase chain reaction定量聚合酶链反应
1.Methods CK19 mRNA in breast cancer cell lines MDA-MB453 and 280 blood specimens (120 patients with breast cancer, 80 with other cancers and 80 normal controls) were detected by real-time quantitative polymerase chain reaction (FQ-PCR), and the detection of GAPDH mRNA was used as control.方法应用逆转录结合荧光定量聚合酶链反应(FQ-PCR)检测乳腺癌MDA-MB453细胞株血液标本模型和280例临床标本的CK19表达,同时检测三磷酸甘油醛脱氢酶(GAPDH)表达作为PCR方法的监控指标。
2.Methods Fluorescence quantitative polymerase chain reaction(PCR) was used to detect the HBV DNA content and enzyme linked immunosorbent assay(ELISA)was used to detect the antigen-antibody in 180 CHB patients.方法采用荧光标记定量聚合酶链反应和酶联免疫吸附实验法检测180例慢性乙型肝炎患者血清HBV DNA含量和抗原抗体,统计分析不同抗原抗体模式HBV DNA含量。
3.Methods The concentration of HCV RNA was detected in 104 patients with HCV infection by means of quantitative polymerase chain reaction technique which was based on a Amplisensor assay.方法 采用荧光定量聚合酶链反应 (PCR)技术检测了 10 4例HCV感染者血清HCVRNA含量。
英文短句/例句
1.The accidence exploration of estimation of curative effect in hepatitis B by fluorescence quantitative PCR荧光定量聚合酶链反应对乙型肝炎患者个体用药疗效的评定
2.The Preparation of Satb2 Gene Standard Plasmids for Real-time Quantitative PCR and Its Application;Satb2基因实时荧光定量聚合酶链反应标准品的制备及应用研究
3.Research on the Application in Diagnosis of Fluorescent Quantitation Polymerase Chain Reaction of Paratyphoid A荧光定量聚合酶链反应诊断甲型副伤寒的应用研究
4.Fluorogenic quantitative PCR method for Plasmodium vivax间日疟原虫荧光定量聚合酶链反应检测方法的建立
5.Monitoring of Minimal Residual Disease (MRD) by Real-time Quantitative Reverse Transcription PCR in Acute Promyelocytic Leukemia;实时荧光定量聚合酶链反应对人急性早幼粒细胞白血病的MRD监测
6.Detection of mutans streptococci in children saliva by real-time fluorescence quantitative polymerase chain reaction methods;实时荧光定量聚合酶链反应检测儿童口腔致龋菌的实验研究
7.Detection of mtDNA 4 977 bp deletion in peripheral whole-blood of healthy adult健康人周围全血线粒体DNA4977bp缺失的实时定量聚合酶链反应检测
8.Comparision of KLK6 expression in breast cancer tissue by two types of fluorescence quantitative PCR modes两种定量聚合酶链反应模式检测乳腺癌组织中缓激肽释放酶表达的比较
9.The assay method used included the Roche Amplicor assay (version 2.0) and real-time PCR.检测方法采用实时荧光定量聚合酶链反应方法和罗氏公司的pcr内标定量方法。
10.Detection of the trachoma chlamydia by fluoroqenic quantitative PCR and super microscope荧光定量聚合酶链反应和超高倍显微诊断系统镜检检测沙眼衣原体
11.Detection of Yersinia enterocolitica in diarrhea stool by real-time PCR实时定量聚合酶链反应检测腹泻粪便中小肠结肠炎耶尔森菌的研究与评价
12.Monitoring of P16 Abnormal Methylation by Real-time Fluorescent Quantitative PCR in NSCLC;实时荧光定量聚合酶链反应检测人肺癌P16抑癌基因启动子异常甲基化
13.Clinical evaluation of FQ-PCR assay for early diagnosis of EBV infection in children by testing EBV-DNA in throat washing荧光定量聚合酶链反应法检测咽拭子EBV-DNA在早期诊断EBV感染中的作用
14.EXPERIMENTAL STUDY ON CALORIMETRY OF POLYMERASE CHAIN REACTION聚合酶链式反应(PCR)量热学的实验研究
15.Research of Real-time Quantitative Reverse-transcriptase Polymerase Chain Reaction (RQ-PCR) to Detect bcr/abl Fusion Gene in Chronic Myeloid Leukemia;实时定量逆转录聚合酶链反应检测慢粒bcr/abl融合基因的研究
16.Detection of mRNA in kidney of dead rat using fluorescence semi-quantitative RT-PCR;荧光半定量逆转录-聚合酶链式反应检测大鼠死后肾mRNA
17.Species identification of animal cells by polymerase chain reaction应用聚合酶链式反应鉴定实验细胞的来源种属
18.Rapid Identification of Acinetobacter baumannii by Multiplex PCR多重聚合酶链反应技术快速鉴定鲍曼不动杆菌
相关短句/例句
quantitative PCR定量聚合酶链反应
1.9% by the quantitative PCR assay,55.目的探讨荧光定量聚合酶链反应(PCR)与普通定性PCR检测血清HBV DNA结果的差异。
3)FQ-PCR荧光定量聚合酶链反应
1.EXPERIMENTAL STUDY ON DETECTION OF HSV-Ⅱ IN PATIENTS WITH STD FROM OUTPATIENT DEPARTMENT WITH FQ-PCR;荧光定量聚合酶链反应检测性病患者感染单纯疱疹病毒Ⅱ的实验研究
2.EXPERIMENTAL STUDY ON DETECTION OF HUMAN PAPILLOMA VIRUSE WITH FLUORESCENT QUANTIVATIVE PCR(FQ-PCR).;荧光定量聚合酶链反应检测人乳头瘤病毒的实验研究
3.Objective To investigate the fluorescence quantitative polymerase chain reaction(FQ-PCR)in the giant cell viral hepatitis in value.目的探讨荧光定量聚合酶链反应(FQ-PCR)在巨细胞病毒性肝炎中的应用价值。
4)Fluorescence quantitative polymerase chain reaction荧光定量聚合酶链反应
1.Detection of mutans streptococci in children saliva by real-time fluorescence quantitative polymerase chain reaction methods;实时荧光定量聚合酶链反应检测儿童口腔致龋菌的实验研究
2.Methods 130 patients urogenital tract secretions and prostatic fluid were collected for Uu detection by both broth method and fluorescence quantitative polymerase chain reaction (FQ-PCR), and bacterial culture was conducted in the Uu positive and polluted liquid medium.方法采集130例患者的泌尿生殖道分泌物﹑前列腺液,同时进行液体培养法和荧光定量聚合酶链反应(FQ-PCR)检测Uu,并对Uu阳性和判为污染的液体培养基进行细菌培养。
3.To investigate the HBV DNA, Fluorescence quantitative polymerase chain reaction (FQ-PCR) was performed on 626 samples.方法用酶联免疫吸附试验(ELISA)对51746例临床标本常规HBV检测,对常见模式组用荧光定量聚合酶链反应(FQ—PCR)检测HBVDNA,对罕见模式组用ELISA进行复检。
5)Real-Time Quantitative polymerase chain reaction实时定量聚合酶链反应
1.The Establishment of Real-time Quantitative Polymerase Chain Reaction Method for Detecting Cardiovascular Bioactive Polypeptide Apelin;实时定量聚合酶链反应检测心血管活性多肽apelin方法的建立
6)Fluorescence quantitative PCR荧光定量聚合酶链反应
1.Methods:NG -DNA in 121 cases of specimens,CT - DNA and UU - DNA in 149 cases of specimens were examined by Fluorescence Quantitative PCR( FQ - PCR).方法:用荧光定量聚合酶链反应(FQ—PCR)检测了121份标本的NG—DNA和149份标本的CT—DNA与UU—DNA。
延伸阅读
链反应链反应chainreaction通过在反应过程中交替和重复产生的活性中间体（自由基或自由原子）而使反应持续进行的一类化学反应。如反应H2＋Cl2→2HCl的机理如下：Cl2＋M→2Cl＋M（1）Cl＋H2→HCl＋H（2）H＋Cl2→HCl＋Cl（3）……………2Cl＋M→Cl2＋M（4）在反应（1）中，靠热、光、电或化学作用产生活性组分——氯原子，随之在反应（2）、（3）中活性组分与反应物分子作用而交替重复产生新的活性组分——氯原子和氢原子，使反应能持续不断地循环进行下去，直到活性组分消失，此即链反应。反应中的活性组分称为链载体。链反应的机理一般包括3个步骤：①链引发。是依靠热、光、电、化学等作用在反应系统中产生第一个链载体的反应，一般为稳定分子分解为自由基的反应，如反应（1）。②链的传递。由链载体与饱和分子作用产生新的链载体和新的饱和分子的反应，如反应（2）、（3）。③链终止。链载体的消亡过程，如反应（4）。式中M为接受链终止所释放出能量的第三体（其他分子或反应器壁等）。在链传递阶段，若一个旧的链载体消失只导致产生一个新的链载体，称为直链反应；若一个旧的链载体消失而导致产生两个或两个以上的新的链载体，则称为支链反应。
