1)duplex PCR二重PCR
1.Development of duplex PCR for detecting Escherichia coli O157:H7 in foods of animal origin;动物性食品中大肠埃希氏菌O157∶H7特异性二重PCR检测方法的建立
2.Establishment of duplex PCR assay for quick differentiating capripoxvirus and orf viruses;羊痘病毒和羊口疮病毒二重PCR鉴别检测方法的建立
3.Developmemt of duplex PCR assay for detection of duck plague virus and goose parvovirus;二重PCR检测鸭瘟病毒和鹅细小病毒的初步研究
英文短句/例句
1.Diagnosing X-linked Ichthyosis by Monoplast Single-round Duplex PCR单细胞单轮二重PCR诊断X-连锁鱼鳞病
2.ABO Genotyping by Duplex Amplification and Oligonucleotide Arrays Assay;运用二重PCR和DNA芯片技术检测ABO基因型
3.Establishment and Application of Double PCR Detection Method for Salmonella and Shigella沙门菌和志贺菌二重PCR检测方法的建立及应用
4.Study on Duplex PCR Diagnosis Method for Escherichia Coli Causing Edema Disease of Swine and Trivalent Inactivated Bacterin;致猪水肿病大肠杆菌二重PCR检测方法及三价灭活菌苗研究
5.Establishment of a duplex PCR for the distinction of porcine circovirus type 1 and type 2鉴别猪圆环病毒1型和2型的二重PCR方法的建立
6.Establishment and application of Duplex PCR Assay for Detection of Streptococcus suis species and Serotypes 9猪链球菌种与9型猪链球菌二重PCR检测方法的建立及应用
7.Studies on specific detection of Listeria monocytogenes in foods by duplex PCR食品中单核细胞增生李斯特菌特异性二重PCR检测方法的研究
8.Isolation and Identification of the Campylobacter Jejuni and Rapid Detection by Mutiplex PCR in Animal Food动物性食品源空肠弯曲杆菌的分离鉴定及其二重PCR检测方法的建立
9.Establishment and application of multiplex PCR on detecting porcine reproductive and respiratory syndrome virus VR2332 and NSP2 strain猪繁殖与呼吸综合征病毒美洲株与NSP2变异株二重PCR诊断方法的建立及应用
10.Application of double orthogonal design to optimize multiplex PCR conditions;二重正交设计用于多重PCR条件优化
11.DEVELOPMENT OF A MULTIPLEX REAL-TIME PCR ASSAY FOR DETECTION OF WSSV AND IHHNVWSSV和IHHNV二重实时荧光PCR检测方法的建立
12.Detection of Escherichia Coli O157 by Duplex Real-Time PCR and Molecular Polymorphic Analysis of O157 Isolates;大肠杆菌O157二重荧光定量PCR检测及分离菌株的分子多态性分析
13.Isolation, Identification of the Highly and Lowly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Establishment a Duplex RT-PCR Technology to Differentiate the Highly and Lowly PRRSV高、低致病性PRRSV的分离鉴定和二重RT-PCR鉴别诊断方法的建立及应用
14.Development and Application of A Duplex RT-PCR Method to Differentiate the PRRSV Variant and Common StrainsPRRSV变异株与普通株二重RT-PCR鉴别方法的建立与应用
15.Development of a multiplex PCR/RT-PCR assay for detection of porcine reproductive and respiratory syndrome virus,porcine parvovirus,pseudorabies virus and porcine circovirus type 2多重PCR/RT-PCR技术检测PRRSV、PPV、PRV和PCV-2
16.Developmemt of a duplex RT-PCR assay for detection of encephalomyocarditis virus and porcine reproductive and respiratory syndrome virus猪脑心肌炎病毒和猪繁殖与呼吸综合征病毒二重RT-PCR检测方法的建立
17.Diagnosis Establishment Formulation of RT-PCR、Fluorescence Quantitative PCR and Mutiplex RT-PCR Detection for Japanese Encephalitis Virus;猪乙脑病毒RT-PCR、荧光定量PCR及多重RT-PCR诊断方法建立
18.Rapid Detection of Avian Influenza Virus by Diplex RT-PCR and Real-Time RT-PCR;禽流感病毒双重RT-PCR及荧光RT-PCR快速检测
相关短句/例句
Double PCR二重PCR
1.The Establishment of a Double PCR Assay for Diagnosis of E.wenyoni and T.evansi Infection;奶牛附红细胞体和伊氏锥虫二重PCR诊断方法的建立
3)duplex RT-PCR二重RT-PCR
1.Development and Application of A Duplex RT-PCR Method to Differentiate the PRRSV Variant and Common StrainsPRRSV变异株与普通株二重RT-PCR鉴别方法的建立与应用
2.Developmemt of a duplex RT-PCR assay for detection of encephalomyocarditis virus and porcine reproductive and respiratory syndrome virus猪脑心肌炎病毒和猪繁殖与呼吸综合征病毒二重RT-PCR检测方法的建立
4)Multiplex(Duplex) PCR多重(二重)PCR
5)two-time duplex PCR二重二次PCR
1.Cytogenetic diagnosis on single lymphocyte of DMD patient with exon 50 deletion by two-time duplex PCR;二重二次PCR对外显子缺失型DMD患者单淋巴细胞遗传学诊断的研究
6)duplex fluorescent PCR二重荧光PCR
延伸阅读
4,4'-二脒基重氮氨基苯二乙尿酸分子式：C22H29N9O6分子量：515.54CAS号：908-54-3性质：黄色结晶性粉末。熔点217℃（分解）。溶于14份20℃的水，微溶于乙醇，难溶于氯仿、乙醚、丙酮。无臭，味苦。制备方法：以对硝基苯甲酸为原料，在高温下与对甲苯磺酰胺发生氰化反应，生成对硝基苯腈（C7H4N2O2[619-72-7]。然后由对硝基苯腈加成、胺化、还原、重氮化、偶合、成盐而得二脒那秦。1.加成、胺化将对硝基苯腈、甲醇、甲醇钠加入反应锅，搅拌溶解，在25-30℃反应3h。加入氯化铵，在10-15℃搅拌反应5h。回收甲醇至干，加入稀盐酸煮沸，过滤，得对硝基苯脒盐酸盐溶液。2.还原将上述溶液加热至50℃，加入冰醋酸，于60℃慢慢加入铁粉。加毕，升温至105℃反应5h。过滤，滤液用氢氧化钠溶液碱化至pH为12。过滤，用盐酸将滤液重新酸化至pH为1-2。浓缩，冷却结晶，过滤，得对氨基苯脒盐酸盐。3.重氮化、偶合先将一半量的对氨基苯脒盐酸盐加入水中，加盐酸溶解，于-5～0℃滴加10%的亚硝酸钠溶液，用碘化钾淀粉试纸检查终点，将另一半量的对氨基苯脒盐酸盐加少量水混匀后加入，再加饱和乙酸钠，析出黄色沉淀。过滤，滤饼溶于水中，用10%氢氧化钠溶液调节pH至9-10，析出结晶。过滤，滤饼用冷水和甲醇洗涤即得1,3-双（对脒基苯）-三氮烯。4.成盐 将上述三氮烯、乙酰甘氨酸、甲醇搅拌0.5h后，用氨水调节至pH为7，升温回渡溶解。加活性炭，过滤，滤液冷却结晶。过滤、干燥，得二脒那秦。用途：兽药。用于治疗大家畜焦虫和锥虫病。常用量，马：3-4mg/kg（体重），牛：4-7mg/kg（体重），羊：3-5mg/kg（体重）。用5%的水溶液，从肌肉或皮下注射。
