1)Enterotoxin gene肠毒素基因
1.Therefore,the enterotoxin genes proved present extensively in Bt strains,suggesting that the pote.采用PCR方法对引自美国俄亥俄州立大学芽孢杆菌遗传保存中心(BGSC)的45个苏云金芽孢杆菌(B t)进行了hblA、bceT及entS 3种蜡状芽孢杆菌(Bc)肠毒素基因的检测。
2.Using boiling and thawing to prepare PCR substrates,the distribution of enterotoxin gene among the 15 Bacillus thuringiensis serovars were detected by specific primers for enterotoxin genes from B.以煮沸冻融法制备PCR扩增模板 ,应用肠毒素 (enterotoxin)基因特异引物EntA和EntB对 15个苏云金杆菌 (Bacillusthuringiensis Bt)血清变种进行PCR扩增分析 ,其中 13个含有肠毒素基因 ,为从分子水平探讨Bt与蜡质芽孢杆菌 (Bacilluscereus,Bc)的肠毒素致病性差异奠定基
3.Screening and identification of the enterotoxin positive strain of Clostridium perfringens A specific pairs of primers were designed and synthesized according to the Clostridium perfringens enterotoxin genes (cpe) from reference report, 34 Clostridium perfringens isolates from Guizhou province were amplified by PCR using the primer.产气荚膜梭菌肠毒素阳性菌株的筛选与鉴定参考相关资料,针对产气荚膜梭菌肠毒素基因设计合成1对特异性引物,对34株贵州分离株进行PCR扩增,并将扩增产物连接到pMD18-T载体,转化至大肠杆菌DH5a感受态细胞,提取质粒进行PCR和双酶切鉴定后测序和分析。
英文短句/例句
1.Cloning, Modification and Expression of Heat-labile Enterotoxin Gene from E.coli大肠杆菌不耐热肠毒素基因的克隆、改造及表达
2.Detection of Enterotoxin Genes of Escherichia coli from Piglets by Multiplex-PCR Assay猪大肠埃希菌肠毒素基因的多重PCR检测
3.Multiplex PCR for Detection of the Three Enterotoxigenic Genes in Enterotoxigenic Escherichia Coli and the Cloning and Expression of Recombinant LTB、LT、LTK63、LTR72;ETEC肠毒素基因多重PCR检测和热敏肠毒素的克隆与表达
4.Entertoxin Genotype Analysis of Staphylococcus Isolates and Expression, Purification of Two Entertoxins葡萄球菌分离株肠毒素基因型分析及两种毒素的表达纯化
5.The Study of Distribution and Enterotoxin Genes of Staphylococcus Aureus;金黄色葡萄球菌的分布及其肠毒素基因的研究
6.Construction of Prokaryotic Expression Vector of the Enterotoxin Gene of Clostridium perfringens Type C产气荚膜梭菌肠毒素基因原核表达载体的构建
7.Study on Detection of Staphylococcus aureus Enterotoxin A Gene by PCRPCR技术检测金黄葡萄球菌肠毒素A基因
8.Detection of Gene Encoding Staphylococcal Enterotoxin A,E by PCRPCR检测金黄色葡萄球菌肠毒素A、E基因
9.Detection of Staphylococcus aureus enterotoxin A gene by RT-PCRRT-PCR检测金黄色葡萄球菌肠毒素A基因
10.Construction and Expression of Fusion Gene of Heat-Labile Enterotoxin B Subunit and K99 Fimbrial of Escherichia Coli;产肠毒素大肠杆菌LTB-K99融合基因的构建与表达
11.Cloning of E. colt Heat-labile Toxin Subunits Genes and Preparation of Their Probes大肠杆菌不耐热肠毒素亚基基因的克隆及其基因探针的制备
12.Cloning and identification of the gene encoding the superantigen staphylococcus enterotoxin A超抗原金黄色葡萄球菌肠毒素A基因克隆与鉴定
13.The Experimental Study on Engineering Antibody of Anti-EHEC O157:H7 Shiga Like Toxin Ⅱ;抗肠出血性大肠杆菌O157:H7志贺样毒素Ⅱ基因工程抗体的实验研究
14.Expression of Fusion Gene of Enterotoxigenic E.coli K88-STII-LTA2/LTB in Transgenic Tobacco;产肠毒素大肠杆菌K88-STII-LTA2/LTB融合基因在烟草中的表达
15.Cloning and Protocaryotic Expression of ETEC K88ac-STⅡ Fused Gene;产肠毒素性大肠杆菌融合基因K88ac-STⅡ的克隆与原核表达
16.Study on Double-gene Co-expression of Fimbrial Subunit Gene K88/K99 and 987P/F41 of Enterotoxigenic Escherichiacoli;猪源产肠毒素性大肠杆菌K88、K99，987P、F41双基因串联表达的研究
17.Prokaryotic Expression of the Escherichia coli Heat-labile Enterotoxin B Subunit Gene大肠杆菌不耐热肠毒素B亚单位基因的原核表达
18.Expression of Fusion Gene of Enterotoxigenic E.coli K88ac-STⅡ-LTA2/LTB in Transgenic Alfalfa产肠毒素大肠杆菌K88ac-STⅡ-LTA2/LTB融合基因在苜蓿中的表达
相关短句/例句
Enterotoxin A gene肠毒素A基因
3)cholera enterotoxin AB gene(ctxAB)霍乱肠毒素基因
4)Enterotoxins C gene肠毒素C基因
5)ST gene耐热肠毒素基因
6)enterotoxin D geneSED肠毒素基因
延伸阅读
肠毒素分子式：CAS号：性质：又称内毒素。能被肠道吸收，直接或间接影响肠黏膜功能的任何外毒素。如霍乱肠毒素、肠道致病性大肠埃希氏菌热敏感毒素、葡萄球菌肠毒素(A, B, Cl，C2,D和E)链球菌外毒素等，它们都是蛋白质或肽。分子量一般在28000～34000，由葡萄球菌产生，作用于内脏，引起痉挛、恶心、腹泻和呕吐。通常感染这类细菌的原因是奶制品或肉类导致的食物中毒。
