免疫捕获RT-PCR,IC-RT-PCR
1)IC-RT-PCR免疫捕获RT-PCR
1.In this study,based on the previously reported viral coat protein(CP)gene sequences,specific primers for ORSV and CyMV were designed,and four methods,including ELISA,RT-PCR,nested RT-PCR and IC-RT-PCR were performed for molecular detection of the two viruses.本研究根据病毒外壳蛋白基因设计特异性引物,应用ELISA、普通RT-PCR、巢式RT-PCR和免疫捕获RT-PCR4种方法进行了检测研究与比较。
2)Immuno-capture nested RT-PCR免疫捕获巢式RT-PCR
3)Multiplex Immuno-capture RT-PCR多联免疫捕获RT-PCR
4)one-step ic-rt-pcr免疫捕获一步RT-PCR
英文短句/例句

1.Detection of Bean Pod Mottle Virus by One-step IC-RT-PCR菜豆荚斑驳病毒免疫捕获一步RT-PCR检测
2.Detection of Salmonella in milk by Combination of Immunomagnetic Separation and PCR免疫磁珠捕获-PCR检测牛乳中沙门氏菌的研究
3.Detection of Food-and-Mouth Disease by RT-PCR and Immune Antibody Surveillance;口蹄疫的RT-PCR检测及免疫抗体监测
4.Development of an Antigen Capture Reverse Transcriptase/Polymerase Chain Reaction for Detection Foot-and-Mouth Disease Virus;抗原捕获反转录聚合酶链式反应(AC-RT/PCR)检测口蹄疫病毒方法的建立
5.Real-time Immuno-RT-PCR for Antigen Detection with Armored RNA as Reporter;一种以armored RNA作为报告基团超灵敏的实时免疫RT-PCR方法的建立
6.Immunobeads Combine RT-PCR Detection of Gastric Cancer Tumor Markers α4GnT;免疫磁珠联合RT-PCR检测胃癌肿瘤标志物α4GnT
7.Detection of Bean pod mottle virus in soybean by one step assay of RT-PCR and real-time fluorescent RT-PCRRT-PCR和实时荧光RT-PCR一步法检测大豆中菜豆荚斑驳病毒
8.One-step RT-PCR for detection of SARS associated coronavirus一步法RT-PCR检测SARS冠状病毒技术研究
9.Detecting HCV RNA by one-step short-product RT-PCR一步短片段RT-PCR方法检测hcv RNA
10.A Study on the one-step RT-PCR for detection of potato Viruses;马铃薯病毒一步法RT-PCR诊断研究
11.Detection of Peripheral Blood Micrometastasis in Patients with Gastric Carcinoma by Combinative Use of Negative and Positive Immunomagnetic Beads with Quantitative RT-PCR;联用阴性和阳性免疫磁珠及定量RT-PCR检测胃癌外周血微转移
12.Establishment of RT-Nested PCR Detection Method about Classical Swine Fever Virus and Study on Immunity and Eradication of CSF in Breeding Swine Field猪瘟病毒套式RT-PCR检测方法的建立和种猪场猪瘟免疫与净化研究
13.Detection of sentinel lymph node micrometastases in cervical cancer patients with RT-PCR and immunohistochemistryRT-PCR与免疫组化法检测宫颈癌前哨淋巴结微转移的探讨
14.Application of immunomagnetic separation in combination with fluorescence quantitative RT-PCR for detecting micrometastasis in circulation in patients with prostate cancer免疫磁珠分选联用实时FQ RT-PCR检测前列腺癌患者外周血微转移
15.Immunocapture-Universal Primer PCR Detection of Salmonella sp. in Foods免疫捕捉-通用引物PCR检测食品中沙门氏菌
16.One-step Duplex RT-PCR Assay for Detection SARS Associated Coronavirus一步法双重RT-PCR检测SARS冠状病毒技术研究
17.Establishment of One-step RT-PCR Detection Method of Live Hepatitis A VirusRT-PCR一步法检测甲型肝炎活病毒方法的建立
18.Detection of the genomic sequences of two alphaviruses by one-step RT-PCR两种甲病毒基因组序列的一步RT-PCR检测
相关短句/例句

Immuno-capture nested RT-PCR免疫捕获巢式RT-PCR
3)Multiplex Immuno-capture RT-PCR多联免疫捕获RT-PCR
4)one-step ic-rt-pcr免疫捕获一步RT-PCR
5)Immunocapture Reverse transcription a免疫捕获反转录聚合酶链式反应(IC-RT-PCR)
6)immunocapture reverse transcription PCR免疫捕获反转录PCR
1.The samples were then analyzed by immunocapture reverse transcription PCR (IC RT PCR) with primers specific for SCMV, Maize dwarf mosaic virus (MDMV), Sorghum mosaic virus (SrMV), and Johnsongrass mosaic virus (JGMV), respectively.利用甘蔗花叶病毒 (Sugarcanemosaicvirus,SCMV)单克隆抗体细胞株 2B5腹水和SCMV、玉米矮花叶病毒 (Maizedwarfmosaicvirus,MDMV)、高粱花叶病毒 (Sorghummosaicvirus,SrMV)和约翰逊草花叶病毒 (Johnsongrassmosaicvirus,JGMV)的特异性引物对我国浙江、江苏、上海、山东、河南、河北、北京、山西、陕西、甘肃、四川、云南 12省市 15个地点的 176株玉米矮花叶病病样分别进行了间接ELISA和免疫捕获反转录PCR(IC RT PCR)检测 ,结果表明这些病样均含有SCMV ,而无MDMV、SrMV或JGMV存在 ,表明上述 12省市的玉米矮花叶病病原为SCMV。
延伸阅读

免疫PCR分子式:CAS号:性质: 通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上,从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上,进而证明有抗原存在。目前最为敏感的检测方法,理论上可测得一个抗原分子的存在。