逆转录聚合酶链反应,Reverse transcriptase polymerase chain reaction英语短句,例句大全

逆转录聚合酶链反应,Reverse transcriptase polymerase chain reaction
1)Reverse transcriptase polymerase chain reaction逆转录聚合酶链反应
1.Methods The p24 gene fragment of BDV in PBMCs or CSFs in these patients infected in intracalvarium were examined by nested reverse transcriptase polymerase chain reaction(RT-PCR) and .方法用荧光定量巢式逆转录聚合酶链反应(FQ-nRT-PCR)方法检测不明原因颅内感染患者及对照者PBMCs或CSFMCs中BDV p24基因片段,同时用β-肌动蛋白(-βactin)作为内参照,并总结阳性患者的临床特征。
2.Methods: The p24 fragment of BDV in peripheral blood mononuclear cells(PBMC) in 52 patients with viral encephalitis and 30 healthy donors were examined by nested reverse transcriptase polymerase chain reaction(RT-PCR) and fluorescence quantitative(FQ) PCR.方法:用荧光定量巢式逆转录聚合酶链反应(FQ-n RT-PCR)方法检测病毒性脑炎患者及正常体检者PB-MCs中BDVp24基因片段,同时用β-肌动蛋白(β-actin)作为内参照,总结出临床特征。
3.The two consecutive fragments of human U5·116 ku were amplified by reverse transcriptase polymerase chain reaction (RT-PCR).方法:从HeLa细胞中提取总体RNA,一步法合成单链cDNA,利用逆转录聚合酶链反应(RT-PCR)法,扩增出人类U5·116 ku基因两段连续的序列,首先分别克隆至pTZ57R/T载体,两段序列连接成全长后再定向克隆至真核表达载体pcDNA3。

英文短句/例句

1.Dection of enterovirus infection by reverse transcription and polymerase chain reaction应用逆转录聚合酶链反应检测肠道病毒感染
2.Rapid identification of subtypes of respiratory syncytial virus by real-time reverse transcription PCR实时逆转录聚合酶链反应快速鉴别呼吸道合胞病毒亚型
3.Research of Real-time Quantitative Reverse-transcriptase Polymerase Chain Reaction (RQ-PCR) to Detect bcr/abl Fusion Gene in Chronic Myeloid Leukemia;实时定量逆转录聚合酶链反应检测慢粒bcr/abl融合基因的研究
4.Expression of the multidrug resistance in human bladder tumors by reverse transcription polymerase chain reaction应用逆转录聚合酶链反应技术检测膀胱癌多项耐药基因的表达
5.Analysis of reserved conditions of blood sample for detection of HCV RNA with RT-PCR逆转录聚合酶链反应检测HCV RNA血标本保存条件分析
6.Comparison Study of Conventional Method and Reverse Transcription Polymerase Chain Reaction on Detection of Flavivirus in Mosquitoes逆转录聚合酶链反应与常规法检测蚊体内黄病毒的比较研究
7.DETECTION OF FISH VIRAL HEMORRHAGIC SEPTICEMIA VIRUS (VHSV) BY NESTED REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION (RT-PCR)应用巢式逆转录聚合酶链反应检测鱼类病毒性出血性败血症病毒(VHSV)
8.Clinical value of antenatal diagnosis of intrauterine active human cytomegalovirus infection with reverse transcription polymerase chain reaction逆转录聚合酶链反应技术用于产前诊断人巨细胞病毒宫内活动性感染的临床价值
9.Establishment of reverse transcription semi-nested polymerase chain reaction to the pathogenic diagnosis of JE patients逆转录-聚合酶链反应在乙型脑炎诊断中的建立
10.Detection of Rice Stripe Virus by Reverse Transcription and Polymerase Chain Reaction逆转录聚合酶链式反应快速检测水稻条叶枯病毒
11.DETECTION OF ENTEROVIRUS RNA IN HUMAN BLOOD SPECIMENS BY REVERSETRANSCRIPION POLYMERASE CHAIN REACTION逆转录聚合酶链式反应检测人血标本中肠道病毒核糖核酸
12.Detection of mRNA in kidney of dead rat using fluorescence semi-quantitative RT-PCR;荧光半定量逆转录-聚合酶链式反应检测大鼠死后肾mRNA
13.Development of a SYBR Green Real-time RT-PCR for Detection of Mumps Virus GeneSYBR Green荧光逆转录-聚合酶链反应检测流行性腮腺炎病毒基因
14.Reverse transcription multiplex-nPCR:application in the study on causative organisms of viral encephalitis in children逆转录多重套式聚合酶链反应在儿童病毒性脑炎病原学研究中的应用
15.Multiplex Reverse Transcription-nPCR: Application in the Study of Causative Organisms of Viral Encephalitis in Children;多重逆转录套式聚合酶链式反应在儿童病毒性脑炎病原学研究中的应用
16.The detection of circulating hepatocellular carcinoma cells in peripheral venous blood by reverse transcription polymerase chain reaction and its clinical significance巢式逆转录多聚酶链反应检测循环性肝癌细胞及其临床意义
17.Studies on the Detection of Bovine Viral Diarrhea-mucosal Disease Virus Using Reverse Transcription-nested Polymerase Chain Reaction;反转录—套式聚合酶链式反应检测牛病毒性腹泻/粘膜病病毒的研究
18.Studies on the Detection and Differentiation of Virulent and Avirulent Strains of Newcastle Disease Virus in Chickens Using Reverse Transcription-Nested Polymerase Chain Reaction;反转录—套式聚合酶链反应检测、鉴别鸡新城疫病毒强、弱毒株的研究

相关短句/例句

RT-PCR逆转录聚合酶链反应
1.METHODS: The Balb/c mice were treated with saline, BCG-PSN and lipopolysaccharide(LPS) respectively, and mIFNγ cDNA was amplified by RT-PCR.方法:分别采用生理盐水、卡介菌多糖核酸和脂多糖处理Balb/c小鼠,逆转录聚合酶链反应(RT-PCR)技术扩增编码小鼠干扰素γ(mIFNγ)cDNA,通过RT-PCR产物来评价卡介菌多糖核酸诱导mIFNγ表达的能力,并设β-actin为对照。
2.RT-PCR was performed for the hTERT mRNA in urine exfoliated cells.方法采用逆转录聚合酶链反应(reverse transcriptase PCR,RT-PCR)检测42例膀胱癌、38例膀胱良性疾病患者和12例健康体检者晨尿中脱落细胞hTERT mRNA的表达,并与尿脱落细胞学检查结果比较;随访观察其中37例膀胱癌术后尿液hTERT mRNA变化与复发的关系。
3.Methods Two siRNAs against the VEGF gene were designed and transfected into T24 cells respectively,VEGF gene expression was measured using RT-PCR.方法体外构建两个针对VEGF基因的siVEGF的重组质粒,转染T24细胞;应用逆转录聚合酶链反应(RT-PCR)测定VEGF基因的表达,并用MTT法和流式细胞仪检测siVEGF对细胞的增殖抑制率和凋亡的影响。
3)reverse transcription polymerase chain reaction逆转录聚合酶链反应
1.[Methods] The level of human Fascin1 gene in endometrial carcinoma tissues(study group,n=30) and normal endometrial tissues(control group,n=10) was investigated by reverse transcription polymerase chain reaction.方法选取30例新鲜子宫内膜癌组织标本(实验组)及10例正常子宫内膜组织标本(对照组),应用逆转录聚合酶链反应(RT-PCR)研究人类Fascin1基因的表达情况,并分析其与组织学类型、临床分期、病理分级、雌孕激素受体状态的相关性。
2.After 1,4,7,10,14 days of exposure,the rats were killed,whole lung RNA were isolated and ATPase6,8 mRNA were detected by reverse transcription polymerase chain reaction(RT-PCR).二组分别于实验后1、4、7、10和14 d提取其肺组织RNA,采用半定量逆转录聚合酶链反应(RT-PCR)测定ATPase6、8 mRNA表达。
3.Methods COX-2 mRNA expression was determined by semi-quantitative reverse transcription polymerase chain reaction(RT-PCR),and cell proliferation analyzed by cell counting kit-8(CCK-8) assay after exposure to dicl.方法采用半定量逆转录聚合酶链反应(RT PCR)检测各种细胞系COX 2 mRNA的表达水平,采用cell counting kit 8(CCK 8)细胞计数法测定药物作用72 h后的细胞增殖活性,绘制生长曲线。
4)reverse transcription-polymerase chain reaction逆转录聚合酶链反应
1.Methods The Reverse transcription-polymerase chain reaction (RT-PCR) with NSE specific primers, Western blot and immunocytochemistry(ICC) were used to check the NSE expression in the tumor cells.方法用NSE特异引物和逆转录聚合酶链反应(RTPCR)、蛋白印迹、免疫细胞化学(ICC)染色技术检测NSE在肿瘤细胞中的表达。
2.3 mRNA in CD4+T cells in different condition was detected by one-step reverse transcription-polymerase chain reaction(RT-PCR) analysis.方法采用一步法逆转录聚合酶链反应(RT-PCR)测定MS急性期患者17例、干扰素(INF--β1b)治疗后MS缓解期患者16例和正常对照10例在不同条件下的CD4+T淋巴细胞上电压依赖性钾离子通道Kv 1。
5)Reverse transcription polymerase chain reaction逆转录酶聚合酶链反应
6)Reverse transcriptase polymerase chain reaction逆转录酶聚合酶链反应