1)HSV1-TK geneHSV1-TK基因
1.Objective:To construct the prokaryotic expression plasmid included HSV1-TK gene and detect the result of expression,the basis for using bacteria vector in tumor gene therapy was made in this work.目的:构建含有HSV1-TK基因的原核表达质粒,并对其原核表达情况进行检测,为进一步将细菌载体应用于肿瘤基因治疗奠定基础。
2.Firstly,plasmid pHSV-106 was taken as the template,the clone of HSV1-TK gene was acquired through PCR and connected to the prokaryotic expression vector pGEX-6P-1,then recombinant plasmid was identified and transformed into E.首先以质粒pHSV-106为模板,通过PCR获得HSV1-TK基因克隆并将其与原核表达载体pGEX-6P-1连接,重组质粒经鉴定后将其转化到E。
2)HSV_1-tk/GCVHSV1-tk/GCV
1.Objective To study whether Topotecan could enhance the killing effect of a suicide gene therapy sy-stem of HSV_1-tk/GCV (herpes simplex ivirus thymidine kinase / ganciclovir ) for ovarian cancer in vivo.目的探讨Topotecan能否增强HSV1-tk/GCV自杀基因系统对卵巢癌的体内治疗作用。
英文短句/例句
1.Experimental Study on the Efficacy of HSV1-tk/GCV System on Human Tongue Cancer Cell Line;HSV1-tk/GCV自杀基因系统对人舌癌细胞作用的实验研究
2.~(18)F-FHBG PET imaging of HSV1-tk reporter gene expression in tumor xenografts of nude mice~(18)F-FHBG PET裸鼠移植瘤的HSV1-tk基因显像
3.Experimental Study of HSV1-tk/FIAU Reporter Gene System Cardiac SPECT ImagingHSV1-tk/FIAU报告基因系统心脏SPECT显像的实验研究
4.The Study of Killing Effect of HSV1-sr39TK/GCV on Gliomas and the Possible Mechanisms of Its Bystander Effect;HSV1-sr39TK/GCV对胶质瘤细胞的杀伤效应及“旁观者效应”机制探讨
5.Study of ATRA Enhancing the Bystander Effect of HSV-TK/GCV Gene System on Bladder Cancer;ATRA增强HSV-TK/GCV对膀胱癌旁观者效应的实验研究
6.A Study of the HSV-TK/GCV Suicide Gene Therapy System in Breast Carcinoma;HSV-TK/GCV自杀基因系统治疗乳腺癌的研究
7.Study on AF-hTERT-TK/GCV Targeted Gene Therapy and Bystander Effect on HCC;AF-hTERT-TK/GCV靶向抗肝癌及旁观者效应的研究
8.The Study of Targetable Killing and Wounding on HCC by hTERT-TK/GCV Combined with hTERT-CD/5-FchTERT-TK/GCV与hTERT-CD/5-Fc联合抗肝癌实验研究
9.The Empirical Study of Hepatocellular Carcinoma's Gene Therapy Using hTERTp-TK/GCV Which was Mediated by Chitosan壳聚糖纳米粒介导的hTERTp-TK/GCV靶向抗肝癌研究
10.Construction of Recombinant Adenovirus Vector with HSV-TK Controlled by hTERT Promoter and Experimental Study of Anti-hepatoma重组Ad-hTERTp-TK/GCV系统的构建及抗肝癌实验研究
11.The relationship of gap junction with the bystander effect in the HSV-TK/GCV treatment细胞缝隙连接与HSV-TK/GCV系统旁观者效应的关系
12.In vitro Study on cytotoxicity of suicide gene HSV-tk/GCV to human laryngocarcinoma cells Hep-2HSV-tk/GCV自杀基因系统对喉癌细胞杀伤作用的体外研究
13.Study of the Inhibited Effects of HSV-TK/GCV System Mediated by rAAV2 on Lens Epithelial Cells;rAAV2介导的HSV-TK/GCV系统对晶状体上皮细胞抑制作用的研究
14.Experimental Studies on the Therapy for Hepatocellular Carcinoma with Adenovirus-mediated Transfer of Thymidine Kinase Gene Driven by IGF-ⅡP3 Promoter and GCV System;IGF-ⅡP3启动子调控腺病毒介导HSV-tk/GCV系统治疗肝癌实验研究
15.Study of p53 Mutation in Tongue Cancer and Experimental Research of Treatment with HSV-TK and IL-2;舌癌p53基因突变及HSV-TK/GCV联合IL-2治疗的实验研究
16.The Enhanceing Effects of Liuwei Dihuang Bolus on Suicide Gene Therapy of Cancer Based on Viral Delivery of Herpes Simplex Virus Type Ⅰ Thymidine Kinase;六味地黄丸对HSV-tk/GCV自杀基因系统治疗肿瘤的增效作用
17.PBCA Nanoparticles-mediated HSV-TK/GCV Gene Therapy for Hepatocellular Carcinoma in Vitro;PBCA纳米粒载体介导的肝癌HSV-TK/GCV基因治疗的体外实验研究
18.Study on rAAV-HSV-TK Gene Therapy of Bladder Cancer with GCV;基因重组HSV-TK腺相关病毒联合GCV治疗膀胱癌的实验研究
相关短句/例句
HSV_1-tk/GCVHSV1-tk/GCV
1.Objective To study whether Topotecan could enhance the killing effect of a suicide gene therapy sy-stem of HSV_1-tk/GCV (herpes simplex ivirus thymidine kinase / ganciclovir ) for ovarian cancer in vivo.目的探讨Topotecan能否增强HSV1-tk/GCV自杀基因系统对卵巢癌的体内治疗作用。
3)HSV1-TK/GCV systemHSV1-TK/GCV系统
4)HSV-TK geneHSV-TK基因
1.Lung carcinoma targeted expression of HSV-TK gene controlled by radioinducible synchronizing positive feedback genetic circuit;放射诱导HSV-TK基因在肺癌细胞中靶向、高效性表达的研究
2.MethodsHuman Tenon s capsule fibroblasts transferred by HSV-tk gene(HTFs-tk) and normal HTFs cells were mixed at a different ratio and seeded at two cell densities,and these cells were exposed to 5 μg/ml ganciclovir.方法将转HSV-tk基因HTFs(HTFs-tk)与正常HTFs于两种接种密度下以不同比例混合,加入5μg/m l更昔洛韦作用5 d,MTT法检测对HTFs的杀伤作用;染料传输法观察HTFs细胞间隙连接细胞间通讯(G JIC)的能力;免疫细胞化学检测Cx43分布;RT-PCR及W estern b lot检测Cx43表达。
3.Single clone harbouring HSV-TK gene was picked after G418 selection.用G418筛选到稳定表达HSV-TK基因的细胞克隆LoVo／LTKSN。
5)tk genetk基因
1.Study on Construction of the homologous recombinant adenovirus tk gene;腺病毒-tk基因同源重组系统构建的研究
2.Construction and identification of recombinant adenovirus with rPB-DR promoter and TK gene;含rPB-DR启动子和TK基因的重组腺病毒的构建和鉴定
3.Relation between immune response and bystander effect when using tk gene to treat therapy glioma;tk基因治疗脑胶质瘤时的免疫反应与旁观者效应的关系
6)HSV tk geneHSV-tk基因
延伸阅读
HSV分子式：CAS号：性质：疱疹病毒中的一种，可以感染人及各种动物。
