Fluorescence quantitative nested reverse transcripate PCR荧光定量套式逆转录酶聚合酶链反应
3)Fluorescence Quantitative nested Reverse Transcriptase PCR荧光定量巢式逆转录酶聚合酶链反应
4)real-time fluorescence relative quantitative RT-PCR实时荧光定量-逆转录-聚合酶链反应
5)real time quantitative RT-PCR实时荧光定量逆转录聚合酶链反应
1.Methods Adult Wistar rats were exposed to hypobaric hypoxic environment for 2 hours following which the expression of AngⅡwere observed at 3 hours,24 hours,3 days,7 days and 14 days with real time quantitative RT-PCR and Western-blotting,respectively.L-1O2)2h后,分别在3h、24h、3d、7d和14d,通过实时荧光定量逆转录聚合酶链反应、蛋白免疫印迹法检测AngⅡ在视网膜中的表达情况。
6)real-time quantitative reverse transcription polymerase chain reaction实时荧光定量逆转录-聚合酶链反应
1.The COL3A1 mRNA was measured by real-time quantitative reverse transcription polymerase chain reaction(RT-PCR).方法应用基因芯片技术和实时荧光定量逆转录-聚合酶链反应(RT-PCR)技术,检测Ⅲ型胶原α链在不同舌苔中的表达水平。
延伸阅读
聚合酶链反应聚合酶链反应polymerase chain reaction,PCR 是用特异性寡核苷酸引物在DNA聚合酶的作用下对靶DNA序列进行大量扩增的分子生物学方法。PCR技术中以在短时间内把极其微量的特定DNA(或RNA)片断大量增扩,达到用常规方法就可以检测的水平。临床用来确定一些疾病的病原体,因其假阳性率较高,故应谨慎使用。
