荧光差异凝胶电泳技术,two-dimensional differential in-gel electrophoresis
1)two-dimensional differential in-gel electrophoresis荧光差异凝胶电泳技术
1.Then two-dimensional differential in-gel electrophoresis (2D-DIGE) was performed to isolate the total proteins of the pooled microdissected cancer cells from non-LNM AdC and LNM AdC.为了筛选潜在的肺腺癌转移相关分子标志物,依据临床诊断选取无转移的肺腺癌组织和有转移的肺腺癌组织作为研究对象,首先采用激光捕获显微切割技术(laser capture microdissection,LCM)对两组肺腺癌组织中的癌细胞进行纯化,再利用荧光差异凝胶电泳技术(two-dimensional differential in-gel electrophoresis,2D-DIGE)分离无转移肺腺癌组和有转移肺腺癌组的癌细胞总蛋白,通过Decyder软件分析两组差异表达的蛋白质点,质谱(mass spectrometry,MS)对差异表达的蛋白质点进行鉴定,Western blot验证部分差异蛋白annexin A1,annexin A2,annexin A3,B23和S100A9的表达。
2)fluorescent two-dimensional difference gel electrophoresis(2D DIGE)荧光差异双向凝胶电泳
3)two-dimensional differece gel electrophresis差异荧光双向凝胶电泳
4)fluorescence two-dimensional differential gel electrophoresis (2D-DIGE)荧光双向差异凝胶电泳
5)two-dimensional fluorescence difference gel eletrophoresis双向荧光差异凝胶电泳
6)differential in-gel electrophoresis(DIGE)荧光差异凝胶电泳
1.In this study,we used differential in-gel electrophoresis(DIGE)and mass spectrometry(MS)to analyze the proteins in U251 cells transfected by pcDNA3.为了进一步了解LRRC4在胶质瘤发生发展中的调控作用,本研究采用荧光差异凝胶电泳(2D-DIGE)和质谱分析技术获得了LRRC4转染U251细胞的11个差异表达蛋白质,并用Western印迹证实了U251细胞在转染LRRC4基因前后热休克蛋白27、stathmin1和S100钙结合蛋白A11的差异表达变化。
英文短句/例句

1.Analysis of Colorectal Cancer by Two-dimensional Differential Gel Electrophoresis结直肠癌组织的二维荧光差异凝胶电泳分析
2.Electrophoresis and fluorospectrophotometry methods to determine the content and entrapment efficiency of siRNA in cationic liposomes凝胶电泳法及荧光光度法测定siRNA阳离子脂质体的含量和包封率
3.Urinary proteomic research based on two dimensional fluorescence differential gel electrophoresis基于荧光差异二维电泳技术的临床尿液蛋白质组学研究方法探讨
4.Proteome study of breast adenofibroma by two-dimensional difference gelelectropnoresls乳腺纤维瘤蛋白质组的凝胶内差异显示电泳分析
5.Proteome Study of Two Different Agkistrodon Venom by Two-dimensional Differential in-gel Electrophoresis两种蝮蛇毒的蛋白质组凝胶内差异显示电泳分析
6.Analysis of Differential Gel Electrophoresis of Paclitaxol Resistant and Sensitive Lung Adenocarcinoma Cells' Secretome紫杉醇耐药细胞与敏感细胞分泌蛋白差异凝胶电泳分析
7.Proteomic analysis was performed by two-dimensional protein gel electrophoresis and subsequent computerized gel analysis for detection of distinguishing patterns of protein expression.蛋白质组学分析是由二维蛋白凝胶电泳和随后的计算机辅助凝胶分析并发现差异表达的蛋白。
8.Establishment of Two-dimension Gel Electrophoresis Protein Profiles and Differential Aanalysis from Human Pituitary Adenoma with and without Invasivness;侵袭性与非侵袭性垂体腺瘤双向凝胶电泳蛋白质图谱的建立及其差异分析
9.pulse alternative field gel electrophoresis脉冲交变电场凝胶电泳
10.Determination of human thrombin by an aptamer based on affinity capillary electrophoresis-laser induced fluorescence detection基于适配体亲和毛细管电泳-激光诱导荧光检测的凝血酶分析
11.polyacrylamide gel electrophoresis聚丙烯酰胺凝胶电泳
12.Pectic Oligosaccharide Analysis by Fluorophore-assisted Carbohydrate Electrophoresis荧光辅助糖电泳用于果胶水解产物中寡糖的分析研究
13.The preparation of nano-aluminate strontium phosphor by sol-gel method溶胶-凝胶法制备纳米铝酸锶荧光粉
14.Study on Preparation of Polymerized Fluorescent Microsphere and Fluorescent Gel聚合物荧光微球及荧光凝胶的制备研究
15.Advances of the fluorescence detection in capillary electrophoresis毛细管电泳荧光检测模式的研究进展
16.PREPARATION AND LUMINESCENT PROPERTIES OF (Y,Ce,Tb)(BO_3,PO_4)溶胶-凝胶法合成Y(BO_3,PO_4):Ce,Tb荧光粉及其荧光性能的研究
17.analytical polyacrylamide gel electrophoresis apparatus分析聚丙酰胺凝胶电泳仪
18.A ladder-like pattern of DNA fragmentation appeared upon agarose gel electrophoresis.琼脂糖凝胶电泳可见“梯状”现象;
相关短句/例句

fluorescent two-dimensional difference gel electrophoresis(2D DIGE)荧光差异双向凝胶电泳
3)two-dimensional differece gel electrophresis差异荧光双向凝胶电泳
4)fluorescence two-dimensional differential gel electrophoresis (2D-DIGE)荧光双向差异凝胶电泳
5)two-dimensional fluorescence difference gel eletrophoresis双向荧光差异凝胶电泳
6)differential in-gel electrophoresis(DIGE)荧光差异凝胶电泳
1.In this study,we used differential in-gel electrophoresis(DIGE)and mass spectrometry(MS)to analyze the proteins in U251 cells transfected by pcDNA3.为了进一步了解LRRC4在胶质瘤发生发展中的调控作用,本研究采用荧光差异凝胶电泳(2D-DIGE)和质谱分析技术获得了LRRC4转染U251细胞的11个差异表达蛋白质,并用Western印迹证实了U251细胞在转染LRRC4基因前后热休克蛋白27、stathmin1和S100钙结合蛋白A11的差异表达变化。
延伸阅读

Sds-聚丙烯酰胺凝胶电泳凝胶电泳sds-聚丙烯酰胺凝胶电泳(sds-page,sodium dodecyl sulfate-polyacrylamide gel electrohoresis)在有去污剂十二烷基硫酸钠存在下的聚丙烯酰胺凝胶电泳。sds-page只是按照分子大小分离的,而不是根据分子所带的电荷和大小分离的。