平板克隆形成法,Colony-forming assay
1)Colony-forming assay平板克隆形成法
2)Clone on dishes平板克隆
3)clone formation克隆形成
1.Methods We detect the multiplication, clone formation, DNA synthesis inhibitive rate and survival rate by liquid cultivation, clone formation, ~ 3 H-TdR addition and MTT colormrtric method.方法通过液体培养法、克隆形成法、3H-TdR掺入法与MTT比色法分别检测给药后肺腺癌细胞的增殖情况、克隆形成率、DNA合成抑制率及生存率来探讨红景天的抑瘤效应。
2.Clone formation assay,BrdU-labeled detain assay and SP cells detecting assay were carried out to analyze the TSCs in SP2/0 cells.以克隆形成试验检测SP2/0细胞中具有形成克隆能力细胞的大体比例;采用BrdU标记滞留试验检测SP2/0细胞中含有DNA永生化链的细胞,即具有干细胞特性的细胞;检测SP2/0细胞中具有干细胞特性的SP细胞存在情况及其比例。
英文短句/例句

1.forming or derived from a single clone.属于单克隆或由单克隆形成
2.The CFE of ESCs was 15.3%,and it was much higher than that in control group (6.7%).表皮干细胞的克隆形成率为 15 3 % ,明显高于对照组的 6 7% ;
3.Comparison of colony-forming capacity of HepG2 cells in different soft agar systemsHepG2细胞在不同琼脂糖浓度下克隆形成能力的比较
4.Objective To clone the human recombined bone morphogenetic protein 2(BMP2) gene in Escherichia coli .目的 :在大肠杆菌中克隆人骨形成蛋白 2基因。
5.The Cloning and Sequencing of Partial Human Bone Morphogenetic Protein 3 Gene;人骨形成蛋白3部分基因的克隆与序列测定
6.Isolation of Psendomonas Fluorescens and Cloning and Functional Analysis of Genes Involved in Biofilm Formation;荧光假单胞菌的分离及生物膜形成相关基因的克隆与功能分析
7.Cloning, Expression, Purification of Human Angiogenin and the Study of Its Antagonists;人血管形成素基因克隆、表达、纯化及其拮抗剂的初步研究
8.Phenoloxidase in the Humoral Fluids of Amphioxus Branchiostoma Belcheri Tsingtauense: Isolation, Purification, Identification, Induction and Gene Cloning;青岛文昌鱼体液酚氧化酶:分离、纯化、鉴定、诱导形成及其基因克隆
9.Molecular Cloning of the Genes Involved in the Wood Formation of Populus Tomentosa Carr. and Their Expression in Tobacco and Poplar;毛白杨木材形成相关基因的克隆及在烟草和杨树中的表达
10.Cloning of PKC Gene Regulating Conidium Germination and Appressorium Formation of Setosphaeria Turcica;调控玉米大斑病菌分生孢子萌发和附着胞形成的PKC基因的克隆
11.cDNA Clone of Blue Color Formation-Related Genes and Construction of Plant Expression Vector;蓝色花形成相关基因cDNA克隆与植物表达载体构建
12.Cloning, Sequencing and Polymorphisic Studying of Pig Bone Morphogenetic Protein-15 Coding Sequence;猪骨形成蛋白15基因编码区序列的克隆、测序及多态性研究
13.Cloning and Expression of Encoding Sequence of Mature Peptide for Bone Morphogenetic Protein-7;人骨形态发生蛋白-7(BMP-7)成熟肽编码序列的克隆与表达
14.Construction of a T-DNA Insertional Library of Coniothyrium Minitans and cDNA Cloning of Two Conidiation Associated Genes;盾壳霉T-DNA插入体库的构建及两个分生孢子形成相关基因的克隆
15.The Construction of Full-Length cDNA Library during the Oil-Formation Phase of the Cotton Seed & the Cloning of pepc Gene棉花种子油脂形成期全长cDNA文库构建与pepc基因克隆
16.cDNA Clone of Blue Color Formation-Related Genes and Selection of Lily Transformated蓝色花形成相关基因cDNA克隆与百合转化品种的选择
17.Study on the Screening of Human Keratinocyte Clones with CCL20 Gene Knockdown and Their Long-term Effects in VitroCCL20基因敲低型人角质形成细胞克隆筛选及其长期体外效应研究
18.In vitro study on the amelioration of T cell-mediated osteoclastogenesis by anti-RANKL-polyclonal antibody抗RANKL多克隆抗体抑制T细胞介导的破骨细胞形成的体外研究
相关短句/例句

Clone on dishes平板克隆
3)clone formation克隆形成
1.Methods We detect the multiplication, clone formation, DNA synthesis inhibitive rate and survival rate by liquid cultivation, clone formation, ~ 3 H-TdR addition and MTT colormrtric method.方法通过液体培养法、克隆形成法、3H-TdR掺入法与MTT比色法分别检测给药后肺腺癌细胞的增殖情况、克隆形成率、DNA合成抑制率及生存率来探讨红景天的抑瘤效应。
2.Clone formation assay,BrdU-labeled detain assay and SP cells detecting assay were carried out to analyze the TSCs in SP2/0 cells.以克隆形成试验检测SP2/0细胞中具有形成克隆能力细胞的大体比例;采用BrdU标记滞留试验检测SP2/0细胞中含有DNA永生化链的细胞,即具有干细胞特性的细胞;检测SP2/0细胞中具有干细胞特性的SP细胞存在情况及其比例。
4)colony formation克隆形成
1.Effect of coixenolide on the colony formation of human nasopharyngeal carcinoma cell line CNE-2Z;薏苡仁酯对人鼻咽癌细胞克隆形成的影响
2.Cell colony formation in soft agar was observed as well.6,克隆形成率为12。
3.Furthermore,after hepatoma cells(Hep3B and HepG2) were treated with different concentrations of Andro(0-30 μmol·L-1) for 14 d,the number of colony formation was accounted under microscope.实验中采用MTT法检测穿心莲内酯(0~50μmol·L·1)对L-02、Hep3B和HepG2细胞存活率的影响,通过软琼脂克隆形成实验检测穿心莲内酯(0~30μmol·L·1)与Hep3B和HepG2细胞孵育14d后对其克隆形成率的影响,通过流式细胞技术检测穿心莲内酯对Hep3B细胞周期的影响。
5)clonogenicity克隆形成
1.By statistical analyses of the clonogenicity indices of phospholipase C γ 1 gene knocked out fibroblast cell line and phospholipase C γ 2 gene vector transfected cell line, we found that phospholipase C γ 2 shew a strong positive regulator effect on the fibroblast induced.通过对敲除磷脂酶 C-γ1基因的细胞株及其转染磷脂酶 C-γ2 基因后克隆形成能力的统计学分析揭示出磷脂酶 C-γ2 有显著的促克隆形成作用 ,磷脂酶 C-γ1作用微小 ,但与磷脂酶 C-γ2 有协同作用 ,提示磷脂酶C-γ2 与 γ1在功能上有一定的交互性、冗余性 ,但不可相互取代 。
6)colony forming efficiency克隆形成率
1.Effects of EGF and bFGF on colony forming efficiency of primary cultured rabbit corneal limbal stem cells;EGF与bFGF单独及联合应用对原代培养的兔角膜缘干细胞克隆形成率的影响
2.The output of viable cells,rates of adherence to dish,colony forming efficiency(CFE) of the isolated epidermal ce.观察不同分离条件对表皮细胞生长情况、贴壁率、克隆形成率的影响。
3.Limiting dilution assay was performed to determine the HL-60 cell colony forming efficiency (CPE).方法应用电子显微镜、琼脂糖电泳、流式细胞术和TdT介导的缺口和末端标记法(TUNEL)等方法分析放射诱导HL-60细胞凋亡,应用有限稀释法测定放射后细胞克隆形成率。
延伸阅读

分子克隆法分子式:分子量:CAS号:性质:见基因工程。