消减筛选,subtractive screening
subtraction biopanning差减筛选
1.Methods Osteosarcoma cells os-732 were used as the target cells and osteoblasts as the absorber cells for subtraction biopanning from a 12-mer peptide phage-display library.方法以骨肉瘤细胞os-732为靶细胞,成骨细胞为吸附细胞对噬菌体12肽库进行差减筛选,用细胞ELISA、免疫组化鉴定阳性噬菌体克隆并测序。
2.Methods With the LoVo cells as the target cells and human normal large intestinal mucosal epithelial cells as the absorber cells for subtraction biopanning from a c7c phage-display peptide library, the positive phage clones were identified by enzyme-linked immunosorbent assay (ELISA) and immuno.方法以大肠癌LoVo细胞株作为靶细胞,人正常结肠黏膜上皮细胞为吸附细胞对噬菌体展示环七肽库进行差减筛选,采用细胞ELISA与免疫荧光检测鉴定噬菌体阳性克隆并测序。
3)counter-selecting减差筛选
4)subtract biopanning减性筛选
5)screening by PCR substrationPCR差减筛选
6)antifoam screening消泡剂筛选
延伸阅读

消减1.减少;降低标准。 2.谓消瘦。