1)hTERT mRNAhTERTmRNA
1.Objective This study was designed to investigate the significance of hTERT mRNA in breast carcinogenesis and to explore the diagnostic efficacy, and to study the effect of tumor suppressor gene p53 on the expression of hTERT mRNA.目的探讨端粒酶hTERTmRNA表达在人乳腺癌发生、发展中的意义,观察肿瘤抑制基因p53与hTERTmRNA表达的关系。
2.Methods Reverse transcription-polymerase chain reaction (RT PCR) method was employed to detect the hTERT mRNA and MDR mRNA in cancer tissues and para tumor liver tissues in 58 paticats with HCC.方法 检测 5 8例HCC患者癌组织及癌旁组织中hTERTmRNA和MDRmRNA的表达情况。
3.Objective To investigate the effect of telomerase activity and hTERT mRNA expression in SMMC7721 cells treated with GDCA in vitro.目的探讨甘氨脱氧胆酸钠(glycodeoxycholic acid,GDCA)对肝癌SMMC7721细胞端粒酶活性以及hTERTmRNA表达影响。
hTERTmRNA端粒酶hTERTmRNA
1.In this study,in order to identify the expression of hTERTmRNA and p53 gene in NSCLC tissues and normal lung tissue,and make clear thei本试验采用原位杂交和免疫组化方法检测端粒酶hTERTmRNA、p53蛋白在非小细胞肺癌(NSCLC)及癌旁正常肺组织中的表达情况,探讨端粒酶hTERTmRNA在非小细胞肺癌组织中的表达及其与p53的相关性。
3)Serotonin[英][,ser?'t??n?n][美]['s?r?'ton?n]5-HT
1.Objective:To observe the influence of electro-acupuncture on the nucleus raphes dorsalis with ultramicroscopic structure change and serotonin(5-hydroxytryptamine,5-HT)level in neurosal rats.目的:通过观察针刺对神经症大鼠中枢内5-HT表达水平的影响和对神经细胞超微结构的影响寻找针刺治疗神经症的证据。
2.Employing a nystatin-perforated patch-clamp whole-cell recording technique, we investigated the modulatory effects of serotonin (5-hydroxytryptamine, 5-HT) on glycine (Gly)-activated whole-cell currents (IGly) in neurons acutely dissociated from the superficial laminae (laminae Ⅰ and Ⅱ ) of the dorsal horn of the spinal cord in the rat.应用制霉菌素穿孔膜片钳全细胞记录技术研究了5-HT对急性分离的大鼠脊髓背角浅层(Ⅰ、Ⅱ层)神经元甘氨酸激活的全细胞电流的调控作用。
3.A triple immunofluorescence histochemical staining technique was used to examine the connections between serotoninlike immunoreactive (5-HT-LI), substance P(SP)-LI terminals and thalamic(TH)- or para brachial (PBN) - projection neurons in the superficial laminae(lamina Ⅰand lamina Ⅱ) of the caudal spinal trigeminal nucleus(Vc) in the rat with con focal laser-scanning microscopy.在Vc浅层内还可观察到大量的5-HT样和SP样免疫荧光组织化学染色阳性纤维和终未,5-HT样阳性纤维有较多的膨体。
4)H-plane tee pairHT对
5)Hash & Tree Tree(HT Tree)HT树
6)HT steel cordHT钢帘线
延伸阅读
端粒酶分子式:CAS号:性质:一种以逆转录的方式合成真核细胞中线性染色体端粒DNA的特殊酶。该酶的辅基为一特定的RNA,端粒DNA的合成必须依赖酶分子中的辅基RNA为模板,通过逆转录而完成,其自身携带RNA作为产物的模板是区别于一般由纯蛋白组成的逆转录DNA聚合酶的主要特征。在逆转录时需要一个DNA引物,以便从5′→3′方向添加端粒的重复序列。底物是脱氧核苷三磷酸(dNTPs)。如在四膜虫(tetraphymena)中,四膜虫端粒酶催化反应式如下:染色体-GGGGTTGGGGTT(DNA引物)+4ndGTP(dNTPs)+2nTTP(dNTPs)→染色体-GGGGTTGGGGTT(GGGGTT)n(四膜虫端粒DNA)+6n焦磷酸端粒酶的活性在胚系细胞、癌化细胞中活跃;在体细胞,尤其经多次分裂后的体细胞,其活性降低。该酶的活性也可能是多细胞器官限制其细胞增殖的一种机制之一。
