1)mutation p53 gene突变型p53基因
1.AIM: To establish bi-transgenic mice models containing mutation p53 gene and Epstein-Barr (EB) virus latent membrane protein 1 gene (LMP1) and to provide reliable tools for studying the reciprocity of mutant p53 with LMP1 in the tumorogenesis process of nasopharyngeal precancerosis.方法:通过分子克隆技术构建含突变型p53基因和EB病毒LMP1基因的真核表达载体pLMP1-p53mt;采用显微注射法将线性化的表达载体注射至小鼠受精卵的雄性原核中,然后将注射存活的受精卵植入假孕母鼠的输卵管;取其产3wk龄子代鼠进行PCR初选,再通过Southern杂交进一步确证;利用HE染色法观察4mo龄转基因小鼠不同组织病理变化。
2.[Objective] To construct eukaryotic expression plasmid containing mutation p53 gene and Epstein-Barr virus latent membrane protein 1 gene (LMP1) so that the reciprocity of mutation p53 with LMP1 in the tumorogenesis process of nasopharyngeal carcinoma could be investigated more easily.目的构建含突变型p53基因和EB病毒LMP1基因的双基因真核表达质粒,为研究突变型p53基因和EB病毒LMP1基因在鼻咽癌发生中的相互作用奠定基础。
英文短句/例句
1.The Study of Dnazyme in Inhibiting Mutant p53 Gene Expression in Vitro;脱氧核酶抑制突变型p53基因表达的体外研究
2.Expressions of VEGF and mutant p53 gene and their clinical significance in patients with gastric carcinoma胃癌中VEGF及突变型p53基因表达及临床意义研究
3.Effect on the Function of 249 Arg to Ser Mutation in the p53 Genep53基因249编码子突变对p53功能的影响
4.Comparative Study between p53 Gene Mutation and Functional Inactivation of p53 in Esophageal Squamous Cell Carcinoma食管鳞癌p53基因突变和p53功能失活的对照研究
5.Experimental Study of p53 Gene Mutations in Keloids;瘢痕疙瘩p53基因突变的实验研究
6.Detection of p53 Gene Mutations of Colorectal Cancer in Guangxi;广西地区大肠癌P53基因突变检测
7.p53 gene mutations in colorectal carcinoma of 48 patients in Guangxi region48例广西结直肠癌p53基因突变分析
8.Expression of hMSH2、hMLH1 and P53 in Sporadic Colorectal Carcinoma;错配修复基因hMSH2、hMLH1与突变型p53在散发性大肠癌中的表达
9.The Study on Regulatory Mechanism of Cell Cycle Block and Apoptosis in p53 Mutated Gastric Cancer Cells during Cisplatin Stress;p53突变型胃癌细胞株细胞凋亡及基因调控机制的研究
10.Study on Relationship and Significance of P16 P53 Rb Expression and P16 P53 Deletion and Mutation in Bladder Transitional Cell Carcinoma;膀胱癌P16、P53、Rb基因表达及P16、P53基因缺失突变的相关性研究
11.Study on the p53 and K-ras Gene Mutations in Gastric Carcinomas;胃癌患者p53基因及K-ras基因的突变研究
12.The Study of Expression of CK20 and the Mutation of p53 Gene in Peripheral Blood of Patients with Colorectal Carcinoma;大肠癌患者外周血CK20表达及p53基因突变的研究
13.Clinical signifcance of p53 gene mutation in circulating plasma DNA from hepatocellular carcinoma patients;原发性肝癌血浆循环核酸p53基因突变的研究
14.A Study on the Expression of FAT10 in Human Gastric Cancer and the Correlation between FAT10 and Mutant p53;胃癌FAT10表达与突变p53基因的相关性研究
15.Mutations in APC,K-ras,p53 and MMR gene in colorectal cancer散发性结直肠癌APC、K-ras、p53、MMR基因突变检测
16.Study on STK15 Gene Overexpression and p53 Gene Mutation in Centrosomal Abnormality of Laryngeal Carcinoma;喉癌p53基因突变和STK15基因过表达与中心体异常相关性研究
17.Influence of common XRCC1 variant alleles on p53 mutations in NSCLC patients非小细胞肺癌患者XRCC1基因多态性对p53基因突变发生的影响
18.The Analysis on p53 Gene Mutation and c-Myc Gene Amplification in Infantile Hemangioma婴幼儿血管瘤组织p53基因突变和c-Myc基因扩增分析
相关短句/例句
Gene mutant p53基因突变型p53
3)p53 gene mutationp53基因突变
1.Analysis of p53 gene mutation in salivary adenoid cystic carcinoma;唾液腺腺样囊性癌p53基因突变及其与临床病理的关系
2.The analysis of p16 gene deletion and p53 gene mutation in colorectal carcinoma;大肠癌p53基因突变及p16基因缺失检测及临床意义
3.Study on the p53 gene mutation and microsatellite instability of gastric carcinoma;胃癌p53基因突变与微卫星DNA不稳定性关系的研究
4)p53 mutation genep53突变基因
5)Mutation P53 gene protein突变型P53基因蛋白
6)mutant p53突变型p53
1.Correlation and potential interaction between FHIT and mutant P53 expression in non-small cell lung cancer;非小细胞肺癌组织FHIT和突变型P53表达的关联性及相互作用
2.Effects of Baicao Jiangzhiling on the Expression of PCNA and Mutant p53 in the Atherosclerotic Rabbit;百草降脂灵对动脉粥样硬化兔腹主动脉PCNA及突变型p53表达的影响
3.The expression and clinical significance of mutant p53,bcl-2,Ki-67,survivin in non-Hodgkin' s lymphoma(NHL)非霍奇金淋巴瘤组织中突变型p53、bcl-2、Ki-67及survivin蛋白表达及临床意义
延伸阅读
琥珀突变型抑制基因分子式：CAS号：性质：又称琥珀突变型抑制基因。是一种能对琥珀密码子(终止密码子，UAG)作出反应，使氨基酸掺入位于mRNA琥珀密码子位点上的多肽链的抑制基因突变。所谓抑制基因突变是指完全或部分恢复初级突变所致功能丧失的次级突变，其突变位点不同于初级突变，不导致初级突变逆转，而是向双重突变体的野生型部分逆转。例如，大肠杆菌的一些菌株的琥珀校正基因，在琥珀密码子位点上可掺入酪氨酸、赖氨酸等一些其他氨基酸，此抑制基因突变发生在为tRNA编写的DNA顺序中的单个碱基取代，突变结果使tRNA的反密码子改变，能在核糖体上与mRNA的UAG配对，如单个碱基突变的酪氨酸tRNA，可读出链终止密码子UAG，如同UAG为酪氨酸的密码子那样。其完整多肽链的出现取决于特定的抑制基因特变，而功能性蛋白质的形成则取决于取代UAG的插入氨基酸。
