1)anti-VEGF hairpin ribozyme抗-VEGF发夹状核酶
1.In this study, we constructed an adenovirus that simultaneously expresses anti-VEGF hairpin ribozyme and human IL-24 and examined its VEGF expression inhibitory effect and anti-tumor efficacy against human colon cancer HT-29 cells in vitro and in vivo.RT-PCR及免疫荧光法检测病毒感染后抗-VEGF发夹状核酶及白细胞介素-24在HT-29细胞中的表达,Real-time PCR及ELISA检测其对VEGF mRNA及蛋白表达的抑制作用,
英文短句/例句
1.Construction of adenovirus expressing anti-vascular endothelial growth factor hairpin ribozyme and its inhibition of growth of colorectal cancer HT-29 cells表达抗-VEGF发夹状核酶腺病毒载体的构建及其对结肠癌生长的抑制作用
2.The Study of Anti-Human VEGF Ribozyme Treatment on Hepatocellular Carcinoma;抗人VEGF发卡状核酶治疗肝癌的实验研究
3.Experimental Study of the Treatment for Ovarian Cancer with Anti-vascular Endothelial Growth Factor Hairpin Ribozyme;抗血管内皮生长因子发夹核酶治疗卵巢癌的实验研究
4.A Double-antibody Sandwich Method of Dot-ELISA for the Detection of Rotavirus双抗体夹心斑点酶联法检测轮状病毒的研究
5.Experimental Study on Inhibitory Effects to RSV Infection by Combination Treatment of Short Hairpin RNA and Deoxyribozyme;小发卡结构状RNA联合脱氧核酶抗呼吸道合胞病毒实验研究
6.The Effect of Short Hairpin RNA of Nuclear Factor Kappa B on the Apoptosis of Hepatic Stellate Cell;核因子-κB亚单位p65短发夹状干扰RNA对肝星状细胞凋亡的影响
7.Antiangiogenesis and Enhancing Function of Leukemic Dendritic Cell by VEGF Antisense Oligodeoxynucleotide;VEGF反义寡核苷酸抗白血病血管新生和增强白血病源性树突状细胞功能的研究
8.The Effects of Recombinant Ad-delivered VEGF Ribozyme on Cell Growth and VEGF Expression of Lung Adenocarcinoma Cell Line A549重组腺病毒介导的VEGF核酶对肺腺癌A549细胞生长及VEGF表达的影响
9.The Research about Interference of Nucleostemin-Specific Short Hairpin RNA in Vitro and in Vivo;核干细胞因子特异性短发夹状RNA的体内外干扰作用研究
10.Effect of Ribozyme Targeting Platelet-derived Growth Factor Receptor βSubunit Gene on Biological Characteristics of Hepatic Stellate Cell;抗PDGF受体β亚单位核酶对肝星状细胞生物学特性的影响
11.Research of cleavage activity of hammerhead ribozyme targeting caspase-7 in primary cultured liver cells抗Caspase-7锤头状核酶在原代肝细胞内的切割活性研究
12.Biosynthesis and function verification of short hairpin RNA targeting to Influenza A virus抗甲型流感病毒短发夹状RNA分子的体外生物合成及功能鉴定
13.Molecular Modeling of Pyrimidine Nucleoside Phosphorylase, Squamous Cell Carcinoma Antigen and Methylenetetrahydrofolate;嘧啶核苷磷酸化酶、鳞状上皮细胞癌抗原以及亚甲基四氢叶酸脱氢/水解酶的分子模拟
14.Detection of Sugarbeet Rhizomania by Double Antibody Sandwich ELISA Assay甜菜丛根病的双抗体夹心酶联免疫吸附测定
15.The Latent Membrane Protein-1 Gene Silence by Combination Treatment of Short Hairpin RNA and Deoxyribozyme in Nasopharyngeal Carcinoma;小发卡状RNA联合脱氧核酶抑制鼻咽癌EBV-LMP1基因表达的实验研究
16.Detection of Serum FT3,FT4,s-TSH,TGAb,TPOAb levels in Patiens with Chronic Urticaria慢性特发性荨麻疹患者血清甲状腺过氧化物酶抗体及甲状腺球蛋白抗体的测定
17.Effect of Anti-HER-2 Ribozyme on Breast Cancer Cells;抗HER-2核酶对乳腺癌细胞的作用
18.Tnhibition of Influenza a Virus Replication by M2 Gene and PB1 Gene Targered Dnazyme in Vitro and Viro脱氧核酶抗甲型流感病毒的效应研究
相关短句/例句
Leukemia cell mode抗VEGF发夹状核酶基因
3)VEGF hairpin ribozymeVEGF发卡状核酶
1.Objective: To observe the effects of recombinant Ad-delivered VEGF hairpin ribozyme(VEGF/Rz) on cell growth and VEGF expression of lung adenocarcinoma cell line A549 in vitro.目的:将携有VEGF发卡状核酶(VEGF/Rz)和绿色荧光蛋白(GFP)的重组腺病毒(Ad-VEGF/Rz-GFP)感染肺腺癌A549细胞,观察VEGF发卡状核酶对肺腺癌A549细胞生长和VEGF表达的影响。
4)Hairpin ribozyme gene发夹状核酶
1.To explore the possibility of inhibiting expression of VEGF in leukemia cells and observe whether the growth, apoptosis and tumorigenesis of cells could be affected after anti-VEGF hairpin ribozyme gene was introduced into the K.目的:运用血管内皮生长因子(Vascular endothelial growth factor,VEGF)抗体阻断白血病K562细胞株VEGF的自分泌作用,探讨其对K562细胞增殖、凋亡及相关基因表达的影响;将携带抗VEGF发夹状核酶基因的真核表达载体转导白血病细胞株K562,建立稳定表达的细胞株克隆,探讨抗VEGF发夹状核酶基因对K562细胞增殖、凋亡等生物学行为的影响及其分子机制;构建血管内皮细胞生长因子受体(VEGFR)Flt-1和KDR特异性短发夹环RNA(shRNA)真核表达载体,并将Flt-1ShRNA导入白血病细胞株K562,观察对白血病细胞Flt-1基因表达的作用,并探讨其对白血病细胞浸润能力的影响及其机制。
5)hairpin ribozyme gene发夹状核酶基因
1.Objective To explore the potential effects of anti-VEGF hairpin ribozyme gene to gene expression profiles in leukemia cell line K562.方法采用脂质体介导的方法将抗VEGF发夹状核酶基因真核表达载体pcDNA-RZ转染白血病细胞株K562、G418抗性筛选获得阳性克隆;抽提基因组DNA,用PCR方法验证核酶基因已转入K562细胞;荧光定量PCR和免疫印迹反应检测白血病细胞中VEGFmRNA和蛋白表达量的改变;应用cDNA微阵列技术检测VEGF基因表达下调对白血病K562细胞株基因表达谱的影响。
6)Fairpin ribozyme发夹型核酶
延伸阅读
抗栓酶 ,蝮蛇抗栓酶药物名称:蝮蛇抗栓酶英文名:Ahylysantinfarctase别名: 抗栓酶 ,蝮蛇抗栓酶 外文名:Ahylysantinfarctase 适应症: 能明显降低血液粘度、血浆纤维蛋白原、血脂,并能减少血小板数量,抑制其粘附和聚集功能。对脑血栓形成有较好疗效,对血栓闭塞性脉管炎、大动脉炎、静脉系统血栓形成、高凝血症等也有效。 用量用法: 静滴:每次每千克体重0.008单位,用等渗盐水或5%葡萄糖溶液250ml稀释后静滴,滴速每分钟以40滴为宜。 不良反应: 乏力,嗜睡,头痛 注意事项: 1.脑出血或有出血倾向者、活动性肺结核、溃疡病、严重高血压、亚急性细菌性心内膜炎、肝肾功能不全者以及月经期妇女忌用。 2.出现出血倾向或过敏反应需立即停药,或用抗蝮蛇血清中和。 规格: 注射用冷冻粉针剂:每支0.25单位。 类别:溶栓药
