实时相对定量PCR,Real-time relative quantitative PCR英语短句,例句大全

实时相对定量PCR,Real-time relative quantitative PCR
1)Real-time relative quantitative PCR实时相对定量PCR
2)relative quantitative real time RT-PCR荧光实时相对定量RT-PCR
1.In this research,the small interfering RNAs(siRNAs) obtained by in vitro transcriptional methods were applied to suppress the expression of chTERT mRNA in MDCC-MSB1 cells by relative quantitative real time RT-PCR,and to evaluate the biological consequences of chTERT down-regulation on M.为通过体外转录的小干扰RNA(Small interference RNA,siRNA)抑制马立克氏病淋巴瘤细胞(MDCC-MSB1)中鸡端粒酶反转录酶(Chicken telomerase reverse transcriptase,chTERT)mRNA的表达,探讨chTERT在MDCC-MSB1中的作用,本试验设计了针对chTERT mRNA的特异siRNA序列(siRNA-1、siRNA-2、siRNA-3),利用T7 RNA聚合酶体外转录系统,制备21 bp的siRNA分子,通过脂质体介导转染MDCC-MSB1细胞,采用荧光实时相对定量RT-PCR方法检测转染细胞chTERT基因mRNA的相对表达量,流式细胞仪测定转染细胞周期变化情况。
3)realtime PCR/RT-PCR实时定量PCR/RT-PCR
4)real-time quantitative PCR实时定量PCR
1.Real-time quantitative PCR in determination of telomere length;荧光实时定量PCR检测端粒长度
2.Detection of IgH rearrangements using real-time quantitative PCR and Its Reaction Parameters;IgH重排的实时定量PCR检测及反应参数研究
3.Principles of real-time quantitative PCR and its application in plant pathology;实时定量PCR的原理及其在植物病理学研究中的应用

英文短句/例句

1.Quantification of DNA Using Improved SYBR Green I RQ-PCR;实时定量PCR检测IgH基因重排的研究
2.Realtime quantitative polymerase chain reaction determining bla_(CTX-M-14) gene in bacterium实时定量PCR检测细菌中bla_(CTX-M-14)基因
3.The Design and Application of The Quantitative Real-time PCR实时定量PCR试验设计原则及应用
4.Real-time Quantitative PCR for Assessing the Bacteria Related to the Excess Sludge Demineralization;影响剩余污泥减量细菌的实时定量PCR研究
5.Real-time PCR Assay for Quantification of Hepatitis B Virus DNA实时定量PCR法测定乙肝病毒脱氧核糖核酸
6.A PCR and Real-time Fluorogenic Quantitative-PCR for Rapid Identification of Bee Chalkbrood Disease;蜜蜂白垩病PCR及荧光实时定量PCR诊断方法的建立
7.Study on Using the Real Time Polymerase Chain Reaction to Quantitate PML-RARα mRNA in APL;应用实时定量PCR方法检测APL PML-RARA mRNA水平的研究
8.Rapid Detection of the 22q11 Deletion with Quantitative Real-time PCR;实时荧光定量PCR快速诊断22q11微缺失
9.Research and Development of Real-time Quantitative RT-PCR for Measurement of Bcr-abl mRNA Transcripts;实时定量RT-PCR检测bcr-abl mRNA的研究
10.Quantitative Analysis of DNA Methylation in Gastric Cancer Using Real-Time Fluorescent Quantitative Polymerase Chain Reaction;实时荧光定量PCR检测胃癌中DNA甲基化
11.Real time PCR quantificational study of DNA extracted by Chelex-100 method;Chelex-100提取生物检材DNA实时PCR定量研究
12.Detection of Potato Blackleg by Real-time Quantitative PCR马铃薯黑胫病菌实时定量荧光PCR检测
13.Reference Gene Selection in Plant Real-time Quantitative Reverse Transcription PCR(qRT-PCR)植物实时荧光定量PCR内参基因的选择
14.Detection and Quantification of Aberrant Leukemia Fusion Gene by Real-time RT-PCR实时定量荧光PCR检测白血病融合基因
15.Development and Data Analysis of Real-Time Fluorescent Quantitative PCR实时荧光定量PCR的发展和数据分析
16.Application of Real-time Fluorescence Quantitative PCR in Plant Disease实时荧光定量PCR在植物病害中的应用
17.Detection of Tilletia controversa Kühn by Real Time Quantitative PCR实时荧光定量PCR鉴定小麦矮腥黑穗菌技术研究
18.Estimating copy number of transgenic gene by real-time fluorescent quantitative PCR(TaqMan)实时荧光定量PCR(TaqMan)法测定外源基因的拷贝数

相关短句/例句

relative quantitative real time RT-PCR荧光实时相对定量RT-PCR
1.In this research,the small interfering RNAs(siRNAs) obtained by in vitro transcriptional methods were applied to suppress the expression of chTERT mRNA in MDCC-MSB1 cells by relative quantitative real time RT-PCR,and to evaluate the biological consequences of chTERT down-regulation on M.为通过体外转录的小干扰RNA(Small interference RNA,siRNA)抑制马立克氏病淋巴瘤细胞(MDCC-MSB1)中鸡端粒酶反转录酶(Chicken telomerase reverse transcriptase,chTERT)mRNA的表达,探讨chTERT在MDCC-MSB1中的作用,本试验设计了针对chTERT mRNA的特异siRNA序列(siRNA-1、siRNA-2、siRNA-3),利用T7 RNA聚合酶体外转录系统,制备21 bp的siRNA分子,通过脂质体介导转染MDCC-MSB1细胞,采用荧光实时相对定量RT-PCR方法检测转染细胞chTERT基因mRNA的相对表达量,流式细胞仪测定转染细胞周期变化情况。
3)realtime PCR/RT-PCR实时定量PCR/RT-PCR
4)real-time quantitative PCR实时定量PCR
1.Real-time quantitative PCR in determination of telomere length;荧光实时定量PCR检测端粒长度
2.Detection of IgH rearrangements using real-time quantitative PCR and Its Reaction Parameters;IgH重排的实时定量PCR检测及反应参数研究
3.Principles of real-time quantitative PCR and its application in plant pathology;实时定量PCR的原理及其在植物病理学研究中的应用
5)Quantitative real-time PCR实时定量PCR
1.287 Gy·min-1;total dose:7 Gy) exposuring,6 months later they were killed,then total RNA and total protein were extracted from thymic lymphomas and normal thymus tissue and cDNA was synthesized,the expressions of c-myc mRNA and protein in thymic lymphomas induced by ionizing radiation and normal thymus were detected by quantitative real-time PCR and Western blotting respectively.min-1,总剂量7Gy)照射BALB/c小鼠,建立小鼠胸腺淋巴瘤模型,6个月后处死小鼠,取辐射诱发小鼠胸腺淋巴瘤和正常胸腺,分别提取总RNA、合成cDNA和提取总蛋白,采用实时定量PCR方法和Western blotting方法分别检测辐射诱发胸腺淋巴瘤和正常胸腺组织c-myc基因mRNA和蛋白表达。
2.The design principles and application in medicine ,molecular biology, food testing of the quantitative real-time PCR were reviewed in this paper.笔者对实时定量PCR试验设计原则及其在医学、分子生物学、食品检测的应用上进行了概述。
6)RT-PCR实时定量PCR
1.Establishment of RT-PCR detecting relative expression of sBD-1-mRNA in Mongolian sheep蒙古绵羊β-防御素基因相对表达水平实时定量PCR方法的建立
2.Method:IGF-IR mRNA expression in marrow cells from patients with MDS and normal controls was quantitatively determined by real time reverse transcript polymerase chain reaction(real time RT-PCR).方法:用实时定量PCR(real time RT-PCR)方法检测胰岛素样生长因子-I受体mRNA的表达量。