1)reverse transcription-nested polymerase chain reaction逆转录巢式聚合酶链反应
2)Fluorescence Quantitative nested Reverse Transcriptase PCR荧光定量巢式逆转录酶聚合酶链反应
3)nested RT-PCR巢式逆转录-聚合酶链式反应
4)Nested RT-PCR巢式逆转录聚合酶链式反应
5)PT-PCR逆转录-巢式聚合酶链反应
6)Nested reverse transcription polymerase chain reaction巢式逆转录聚合酶链反应
1.cDNA was then analyzed by nested reverse transcription polymerase chain reaction (nested RT PCR) using two pairs of primers.方法 :应用激光显微切割技术 (lasermicrodissection)从冰冻切片上将单个结肠细胞切下 ,提取总RNA ,将RNA逆转录成cDNA ,采用巢式逆转录聚合酶链反应 (nestedRT PCR)检测mRNA的表达。
英文短句/例句
1.DETECTION OF FISH VIRAL HEMORRHAGIC SEPTICEMIA VIRUS (VHSV) BY NESTED REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION (RT-PCR)应用巢式逆转录聚合酶链反应检测鱼类病毒性出血性败血症病毒(VHSV)
2.The detection of circulating hepatocellular carcinoma cells in peripheral venous blood by reverse transcription polymerase chain reaction and its clinical significance巢式逆转录多聚酶链反应检测循环性肝癌细胞及其临床意义
3.Dection of enterovirus infection by reverse transcription and polymerase chain reaction应用逆转录聚合酶链反应检测肠道病毒感染
4.Detection of Rice Stripe Virus by Reverse Transcription and Polymerase Chain Reaction逆转录聚合酶链式反应快速检测水稻条叶枯病毒
5.DETECTION OF ENTEROVIRUS RNA IN HUMAN BLOOD SPECIMENS BY REVERSETRANSCRIPION POLYMERASE CHAIN REACTION逆转录聚合酶链式反应检测人血标本中肠道病毒核糖核酸
6.Detection of mRNA in kidney of dead rat using fluorescence semi-quantitative RT-PCR;荧光半定量逆转录-聚合酶链式反应检测大鼠死后肾mRNA
7.Establishment of reverse transcription semi-nested polymerase chain reaction to the pathogenic diagnosis of JE patients逆转录-聚合酶链反应在乙型脑炎诊断中的建立
8.The Detection of Carcinoma Cells in the Peritoneal Washings of the Patients with Gastric Carcinoma by Nested Reverse Transcription-polymerase Chain Reaction and Its Clinical Significance;巢式逆转录酶链反应检测腹腔胃癌细胞及其临床意义
9.Multiplex Reverse Transcription-nPCR: Application in the Study of Causative Organisms of Viral Encephalitis in Children;多重逆转录套式聚合酶链式反应在儿童病毒性脑炎病原学研究中的应用
10.Rapid identification of subtypes of respiratory syncytial virus by real-time reverse transcription PCR实时逆转录聚合酶链反应快速鉴别呼吸道合胞病毒亚型
11.Research of Real-time Quantitative Reverse-transcriptase Polymerase Chain Reaction (RQ-PCR) to Detect bcr/abl Fusion Gene in Chronic Myeloid Leukemia;实时定量逆转录聚合酶链反应检测慢粒bcr/abl融合基因的研究
12.Expression of the multidrug resistance in human bladder tumors by reverse transcription polymerase chain reaction应用逆转录聚合酶链反应技术检测膀胱癌多项耐药基因的表达
13.Reverse transcription multiplex-nPCR:application in the study on causative organisms of viral encephalitis in children逆转录多重套式聚合酶链反应在儿童病毒性脑炎病原学研究中的应用
14.Analysis of reserved conditions of blood sample for detection of HCV RNA with RT-PCR逆转录聚合酶链反应检测HCV RNA血标本保存条件分析
15.Comparison Study of Conventional Method and Reverse Transcription Polymerase Chain Reaction on Detection of Flavivirus in Mosquitoes逆转录聚合酶链反应与常规法检测蚊体内黄病毒的比较研究
16.Development of a SYBR Green Real-time RT-PCR for Detection of Mumps Virus GeneSYBR Green荧光逆转录-聚合酶链反应检测流行性腮腺炎病毒基因
17.Studies on the Detection of Bovine Viral Diarrhea-mucosal Disease Virus Using Reverse Transcription-nested Polymerase Chain Reaction;反转录—套式聚合酶链式反应检测牛病毒性腹泻/粘膜病病毒的研究
18.Studies on the Detection and Differentiation of Virulent and Avirulent Strains of Newcastle Disease Virus in Chickens Using Reverse Transcription-Nested Polymerase Chain Reaction;反转录—套式聚合酶链反应检测、鉴别鸡新城疫病毒强、弱毒株的研究
相关短句/例句
Fluorescence Quantitative nested Reverse Transcriptase PCR荧光定量巢式逆转录酶聚合酶链反应
3)nested RT-PCR巢式逆转录-聚合酶链式反应
4)Nested RT-PCR巢式逆转录聚合酶链式反应
5)PT-PCR逆转录-巢式聚合酶链反应
6)Nested reverse transcription polymerase chain reaction巢式逆转录聚合酶链反应
1.cDNA was then analyzed by nested reverse transcription polymerase chain reaction (nested RT PCR) using two pairs of primers.方法 :应用激光显微切割技术 (lasermicrodissection)从冰冻切片上将单个结肠细胞切下 ,提取总RNA ,将RNA逆转录成cDNA ,采用巢式逆转录聚合酶链反应 (nestedRT PCR)检测mRNA的表达。
延伸阅读
聚合酶链式反应分子式：CAS号：性质：又称基因体外扩增技术或核酸体外扩增技术。利用两种与相反链杂交并利用附着于目标DNA两端的寡核苷酸引物在高温(95℃)使含目标DNA片段的DNA双链变性，分解为单链。在较低温度(37～63℃)与引物退火：然后变温到72℃左右，在耐高温DNA聚合物酶作用下引物被沿样板DNA单链延伸合成互补链，然后又变性→退火→延伸反复进行。引物大大过量，dNTP过量，酶在高温中是较稳定的，这样经过几十个循环，目标DNA片段就按2n数递增。用此法可从mRNA中，cDNA库中扩增出目标基因。过去一般合成500～1000bp较好，现已发展出大片段(75kb)的PCR，且差错甚少。PCR技术的发展还可用于定点突变，质粒重组及FLP等方面。这一技术的作用范围日益扩大，已成为分子生物学工作者基本技术之一。这项专利技术1985年由美国塞特斯(Cetus)公司开发。是20世纪80年代分子生物学领域中的一项革命性突破，已在分子生物学、医学、法学等领域发挥了极大的作用。
