sex identification PCR性别鉴定PCR
3)competitive quantitative RT-PCR竞争性定量RT-PCR
4)quantitative competitive RT-PCR定量竞争性RT-PCR
5)strand-specific real-time RT-PCR链特异性荧光定量RT-PCR
1.The methods of MTT,fluorescence staining of Hoechst 33258,TdT-mediated dUTP nick-end-labeling(TUNEL),flow cytometry and strand-specific real-time RT-PCR were used to assay the inhibitory effects of FMDV on the growth of BHK-21 cells,the morphological features of apoptotic cells,the apoptosis apex,the cell cycle and the RNA product of replication system in cells respectively.试验利用口蹄疫病毒感染BHK-21细胞,通过MTT法、Hoechst 33258染色、原位末端标记技术(TUNEL)、流式细胞术和链特异性荧光定量RT-PCR,分别就口蹄疫病毒对BHK-21细胞生长的抑制作用、凋亡细胞的形态学和分子生物学特征、凋亡峰的出现和细胞周期的变化以及口蹄疫病毒基因组在BHK-21细胞内的复制情况进行了检测。
6)quantitative methylation-specific PCR甲基化特异性定量PCR
1.Setup of quantitative methylation-specific PCR (qMSP) system for ID4 gene theoretically improves the specificity and sensitivity of methylation detection assays, and might play an important role in clinical practice, such as acute leukemia minimal residual disease (MRD) detection.ID4甲基化特异性定量PCR方法的建立从理论上可以提高甲基化检测水平的特异性和敏感性,在微小残留病变检测、复发风险评估、治疗策略选择等方面具有重要的临床意义。
延伸阅读
immune PCR分子式：CAS号：性质： 通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上，从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上，进而证明有抗原存在。目前最为敏感的检测方法，理论上可测得一个抗原分子的存在。
