1)Disulfide stabilized single chain Fv fragments(scdsFv)二硫键稳定性单链抗体
2)ScdsFv单链二硫键稳定抗体
1.Recombinant design and expression of human anti-rabies virus ScdsFv;人源抗狂犬病毒单链二硫键稳定抗体的重组设计与表达
2.Objective:To modify the Arg codons of human anti-GPRV(glycoprotein of rabies virus) ScdsFv,to express ScdsFv in Pichia pastoris and study its bioactivity.目的:对人源抗狂犬病毒糖蛋白(GPRV)单链二硫键稳定抗体(ScdsFv)进行精氨酸密码子修饰,实现其在酵母中的分泌表达,并检测其生物学活性。
英文短句/例句
1.Construction and Biological Activities Research of Single-chain Disulfide-stabilized Fv Targeted Superantigen SEA (D227A);单链二硫键稳定抗体靶向超抗原SEA（D227A）分子的构建与活性研究
2.Preparation and Evaluation of Disulfide-Stabilized Single-Chain Antibody B3(ds-scFv) Targeted SEA;二硫键稳定单链抗体B3（ds-scFv）靶向超抗原SEA的制备及活性鉴定
3.Prokaryotic Expression and Biological Activity of Disulfide-Stabilized TNF-α Single Chain Antibody二硫键稳定的抗TNF-α单链抗体的原核表达及生物学特性鉴定
4.Expression and Characterization of Anti-HIV-1 gp41 dsFv and Recombinant Directing Toxin;抗HIV-1 gp41二硫键稳定单链抗体及其重组导向毒素的表达及活性测定
5.Construction and expression of disulphide stabilized anti-CD3/anti-Pgp diabody二硫键稳定的抗CD3/抗Pgp微型双功能抗体的构建、表达及活性测定
6.Construction, Expression and Characterization of Disulfide Stabilized Fv Fragment Antibody Against Human Lipopolysaccharide Binding Protein;抗人脂多糖结合蛋白二硫键稳定性Fv抗体的构建表达及生物学活性的初步研究
7.Gene Construction, Expression and Biological Activity Analyses of Disulfide Stabilized Fv Fragment Against Xanthomonas Axonopodis pv. Citri柑桔溃疡病菌二硫键稳定Fv抗体基因的构建及产物生物学特性研究
8.Establishment a Cell Line Expressing EGFR Stably and Selection, Characterization of an Internalizing EGFR scFv;稳定表达EGFR细胞系的建立及其抗EGFR单链抗体的筛选与鉴定
9.Expression, Purification and Preliminary Identification of Single Chain Fv Antibody Dimer Against Human Hepatocellular Carcinoma;抗肝癌噬菌体单链抗体二聚体的表达、纯化及初步鉴定
10.Screening and Identification of Phage Antibody of Single Chain Variable Fragment of Human Hepatocellular Carcinoma抗肝癌噬菌体单链抗体的筛选与鉴定
11.Construction of human phage display antibody ScFv library and identification of antibody ScFv against lung adenocarcinoma抗人肺癌单链抗体噬菌体库的构建及特异性单链抗体的鉴定
12.Molecular Directed Evolution of Reshaped Anti-CD3 Single Chain Fv Fragment;抗CD3改型单链抗体的分子定向进化
13.Construction, Expression and Characterization of scFv and BsFv Against Transferrin Receptor;抗TfR单链抗体及其双价抗体的构建、表达及鉴定
14.Expression,purification and biological activity assay of humanized single-chain Fv dimers against hepatocellular carcinoma in Pichia pastoris特异性抗肝癌单链抗体二聚体在毕赤酵母中的表达、纯化及生物学活性鉴定
15.Screening and identification of antibody single chain variable fragment against HIV gp120HIV-gp120人源单链可变区抗体筛选与鉴定
16.Serological Identification of Phage Antibody of Single Chain Variable Fragment of Humam Hepatocellular Cell Carcinoma;抗肝癌噬菌体单链抗体的血清学鉴定研究
17.Preparation and Identification of Monoclonal Antibodies Against Human Stathmin抗人微管不稳定蛋白单克隆抗体的制备及鉴定
18.The Stability of Micro-Carbonyl Sulfide in Carbon Dioxide二氧化碳中微量氧硫化碳标准气体的稳定性
相关短句/例句
ScdsFv单链二硫键稳定抗体
1.Recombinant design and expression of human anti-rabies virus ScdsFv;人源抗狂犬病毒单链二硫键稳定抗体的重组设计与表达
2.Objective:To modify the Arg codons of human anti-GPRV(glycoprotein of rabies virus) ScdsFv,to express ScdsFv in Pichia pastoris and study its bioactivity.目的:对人源抗狂犬病毒糖蛋白(GPRV)单链二硫键稳定抗体(ScdsFv)进行精氨酸密码子修饰,实现其在酵母中的分泌表达,并检测其生物学活性。
3)dsFv二硫键稳定单链抗体
1.Expression and Characterization of Anti-HIV-1 gp41 dsFv and Recombinant Directing Toxin;抗HIV-1 gp41二硫键稳定单链抗体及其重组导向毒素的表达及活性测定
4)Disulfide-stabilized single chain fragment variable(ds-scFv)二硫键稳定的单链抗体
5)disulfide-stabilized Fv fragment二硫键稳定性Fv抗体
1.Construction and expression of human disulfide-stabilized Fv fragment gene to Rabies Virus;人源抗狂犬病毒二硫键稳定性Fv抗体片段基因的构建与表达
6)disulfide-stabilized antibody二硫键稳定性抗体
延伸阅读
二硫键
二硫键
二硫键disulfide bond又称s－s键。是2个sh基被氧化而形成的—s—s—形式的硫原子间的键。在生物化学的领域中，通常系指在肽和蛋白质分子中的半胱氨酸残基中的键。此键在蛋白质分子的立体结构形成上起着一定的重要作用。为了确定蛋白质的一级结构，首先必须将二硫键打开，使成为线状多肽链。为此，需要在2-巯-乙醇、二硫苏糖类、巯基乙酸等的硫化合物与尿素那样的变性剂同时存在下使之发生作用，使还原成sh基（为了防止再氧化通常用适当的sh试剂将该基团烷基化）或是在过甲酸的氧化作用下衍生成-so3h基或是采用在氧化剂共存下用亚硫酸的作用诱导成-s-so3h基的方法。
